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|Antigen||V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) Antibodies|
|Conjugate||This Crk antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA)
|5 references available|
|Supplier||Log in to see|
Product Details anti-Crk AntibodyTarget Details Crk Application Details Handling References for anti-Crk antibody (ABIN358272) Images
|Specificity||This antibody detects CRK pSer41. Predicted to cross react with Mouse (100 % Antigen Homology).|
|Purification||Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S41 of human CRK.|
Target Details CrkProduct Details anti-Crk Antibody Application Details Handling References for anti-Crk antibody (ABIN358272) Images back to top
|Alternative Name||CRK (CRK Antibody Abstract)|
|Background||CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. It has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.Synonyms: Adapter molecule crk, Proto-oncogene C-crk, p38|
|Molecular Weight||22906 Da|
|Pathways||Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-Crk Antibody Target Details Crk Handling References for anti-Crk antibody (ABIN358272) Images back to top
ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Crk Antibody Target Details Crk Application Details References for anti-Crk antibody (ABIN358272) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-Crk antibody (ABIN358272)Product Details anti-Crk Antibody Target Details Crk Application Details Handling Images back to top
Bougnères, Girardin, Weed, Karginov, Olivo-Marin, Parsons, Sansonetti, Van Nhieu: "Cortactin and Crk cooperate to trigger actin polymerization during Shigella invasion of epithelial cells." in: The Journal of cell biology, Vol. 166, Issue 2, pp. 225-35, 2004
Stoletov, Gong, Terman: "Nck and Crk mediate distinct VEGF-induced signaling pathways that serve overlapping functions in focal adhesion turnover and integrin activation." in: Experimental cell research, Vol. 295, Issue 1, pp. 258-68, 2004
Miller, Chen, Gharib, Wang, Thomas, Misek, Giordano, Yee, Orringer, Hanash, Beer: "Increased C-CRK proto-oncogene expression is associated with an aggressive phenotype in lung adenocarcinomas." in: Oncogene, Vol. 22, Issue 39, pp. 7950-7, 2003
Sun, Barbieri: "Pseudomonas aeruginosa ExoT ADP-ribosylates CT10 regulator of kinase (Crk) proteins." in: The Journal of biological chemistry, Vol. 278, Issue 35, pp. 32794-800, 2003
Zhang, He, Zhou, Liu: "Requirement of the p130CAS-Crk coupling for metastasis suppressor KAI1/CD82-mediated inhibition of cell migration." in: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 27319-28, 2003
ImagesProduct Details anti-Crk Antibody Target Details Crk Application Details Handling References for anti-Crk antibody (ABIN358272) back to top