anti-Human Cytoplasmic Protein NCK1 antibody for ELISA

Recommended Cytoplasmic Protein NCK1 Antibody (supplied by: Log in to see )

Antigen
NCK Adaptor Protein 1 (NCK1) Antibodies
  • NCK
  • NCKalpha
  • nck-1
  • NCK1
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • Nck-1
  • Nck1
  • nck
  • nck1-a
  • nck1-b
  • nckalpha
  • NCK adaptor protein 1
  • non-catalytic region of tyrosine kinase adaptor protein 1
  • NCK adaptor protein 1b
  • NCK adaptor protein 1 L homeolog
  • NCK1
  • Nck1
  • nck1b
  • nck1.L
Epitope
AA 185-294
21
20
11
9
7
4
4
3
2
2
2
1
1
1
1
1
Reactivity
Human
132
36
27
7
3
3
3
3
2
2
2
2
2
1
1
1
Host
Mouse
87
44
1
Clonality
Monoclonal
Conjugate
This Cytoplasmic Protein NCK1 antibody is un-conjugated
5
5
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
130
77
54
14
13
12
8
6
5
2
1
1
1
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Catalog No. ABIN393431
$ 461.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.166649 ABIN4880888 ELISA FACS IHC WB Mouse IgG1 Log in to see 5B7 0
12.166649 ABIN969545 FACS IHC ELISA WB Mouse IgG1 Log in to see 5B7 2
12.166649 ABIN4338263 CyTOF ELISA FACS IHC WB Mouse IgG1 Log in to see 5B7 0
12.166649 ABIN1845767 FACS IHC ELISA WB Mouse IgG1 Log in to see 0
12.166649 ABIN561929 ELISA WB Mouse IgG1 kappa AA 185-294, partial Log in to see 1A1 0
1 ABIN1499651 ELISA FACS Mouse IgG1 Log in to see 5B7 0
1 ABIN957751 ELISA IHC IHC (p) WB Mouse IgG1, kappa Log in to see 11D10 0
1 ABIN4338266 ELISA WB Mouse IgG3 kappa AA 1-377 Log in to see 6B3 0
1 ABIN6263537 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1724470 IHC (p) ELISA WB Mouse IgG1 kappa Log in to see 11D10 0
1 ABIN1836463 ELISA WB Mouse IgG3, kappa Log in to see AT6B3 0
1 ABIN5774107 ELISA IHC (p) WB Mouse IgG1 kappa Log in to see 11D10 0
1 ABIN2318004 ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN1828445 ELISA WB Mouse IgG1, kappa AA 185-294 Log in to see 1A1 0
1 ABIN1916306 ELISA WB PE Rabbit IgG AA 3-29 Log in to see Polyclonal 0
1 ABIN1916302 ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 3-29 Log in to see Polyclonal 0
1 ABIN1916303 ELISA WB APC Rabbit IgG AA 3-29 Log in to see Polyclonal 0
1 ABIN1916304 ELISA WB Biotin Rabbit IgG AA 3-29 Log in to see Polyclonal 0
1 ABIN1916305 ELISA WB FITC Rabbit IgG AA 3-29 Log in to see Polyclonal 0
1 ABIN221538 IP ELISA WB Rabbit IgG Gly300 Log in to see 0

General

Antigen NCK Adaptor Protein 1 (NCK1) Antibodies
Epitope AA 185-294
(21), (20), (11), (9), (7), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(132), (36), (27), (7), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(87), (44), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cytoplasmic Protein NCK1 antibody is un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(130), (77), (54), (14), (13), (12), (8), (6), (5), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Cytoplasmic Protein NCK1 Antibody

Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN393431) Images
Specificity NCK1 (AAH06403, 185 a.a. ~ 294 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: NCK1 (AAH06403, 185 a.a. ~ 294 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 1A1
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details Cytoplasmic Protein NCK1

Product Details anti-Cytoplasmic Protein NCK1 Antibody Application Details Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN393431) Images back to top
Antigen
Alternative Name NCK1 (NCK1 Antibody Abstract)
Background

Other Names: Cytoplasmic protein NCK1, NCK adaptor protein 1, Nck-1, SH2/SH3 adaptor protein NCK-alpha, NCK1, NCK

Gene Description: NCK adaptor protein 1

Gene Summary: The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. [provided by RefSeq

Molecular Weight 42864 DA
Gene ID 4690
NCBI Accession NP_001177725, NP_006144
UniProt P16333
Pathways TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN393431) Images back to top
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.

Restrictions For Research Use only

Handling

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details References for anti-Cytoplasmic Protein NCK1 antibody (ABIN393431) Images back to top
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-Cytoplasmic Protein NCK1 antibody (ABIN393431)

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling Images back to top
Background publications

Barda-Saad, Shirasu, Pauker, Hassan, Perl, Balbo, Yamaguchi, Houtman, Appella, Schuck, Samelson: "Cooperative interactions at the SLP-76 complex are critical for actin polymerization." in: The EMBO journal, Vol. 29, Issue 14, pp. 2315-28, 2010

Davila, Froeling, Tan, Bonnard, Boland, Snippe, Hibberd, Seielstad: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010

Gehmlich, Hayess, Legler, Haebel, Van der Ven, Ehler, Fürst: "Ponsin interacts with Nck adapter proteins: implications for a role in cytoskeletal remodelling during differentiation of skeletal muscle cells." in: European journal of cell biology, Vol. 89, Issue 5, pp. 351-64, 2010

Preisinger, Kolch: "The Bcr-Abl kinase regulates the actin cytoskeleton via a GADS/Slp-76/Nck1 adaptor protein pathway." in: Cellular signalling, Vol. 22, Issue 5, pp. 848-56, 2010

Voss, Lettau, Janssen: "Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening." in: BMC immunology, Vol. 10, pp. 53, 2009

Images

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN393431) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-NCK Adaptor Protein 1 (NCK1) (AA 185-294) antibody (ABIN393431) anti-NCK Adaptor Protein 1 (NCK1) (AA 185-294) antibody
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) (AA 185-294) antibody (ABIN393431) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) (AA 185-294) antibody (ABIN393431) NCK1 monoclonal antibody (M01), clone 1A1 Western Blot analysis of NCK1 expression in...
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) (AA 185-294) antibody (ABIN393431) Western Blot analysis of NCK1 expression in transfected 293T cell line by NCK1 monocl...
ELISA image for anti-NCK Adaptor Protein 1 (NCK1) (AA 185-294) antibody (ABIN393431) Detection limit for recombinant GST tagged NCK1 is approximately 0.1ng/ml as a captur...