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|Antigen||GATA Binding Protein 3 (GATA3) Antibodies|
|Epitope||AA 36-85 Alternatives|
|Reactivity||Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio) Alternatives|
|Conjugate||This GATA3 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-GATA3 AntibodyTarget Details GATA3 Application Details Handling Images
|Predicted Reactivity||Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%) Guinea pig (92%) Zebrafish (84%).|
Synthetic peptide located between aa36-85 of human GATA3 (P23771, NP_002042). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Zebra finch, Chicken (100%), Bat, Guinea pig, Trout, Salmon (92%), Zebrafish (84%).
Type of Immunogen: Synthetic peptide
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|Alternative Name||GATA3 (GATA3 Antibody Abstract)|
Name/Gene ID: GATA3
Family: Transcription factor
Synonyms: GATA3, GATA-binding factor 3, HDRS, GATA binding protein 3, GATA-binding protein 3, HDR
|Pathways||Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation|
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Approved: IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
|Restrictions||For Research Use only|
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|Buffer||Lyophilized from PBS with 2 % sucrose|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
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