anti-Human ITK antibody for Immunohistochemistry
Recommended ITK Antibody (supplied by: Log in to see )
- Antigen
-
IL2-Inducible T-Cell Kinase (ITK) Antibodies
show synonyms for this antigen- Emt
- Tcsk
- Tsk
- EMT
- LPFS1
- LYK
- PSCTK2
- IL2 inducible T cell kinase
- IL2 inducible T-cell kinase
- IL2-inducible T-cell kinase
- Itk
- ITK
- Epitope
-
pTyr512
Alternatives - Reactivity
-
Human, Mouse (Murine)
Alternatives - Host
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Rabbit
Alternatives - Clonality
- Conjugate
-
This ITK antibody is un-conjugated
Alternatives - Application
-
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Alternatives - Supplier
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- Supplier Product No.
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| Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|---|
| 15.579585 | ABIN1589997 | ELISA IHC WB | Goat | Internal Region | Log in to see | Polyclonal | 0 | |||
| 9.970049 | ABIN609692 | IHC IHC (p) | Goat | AA 596-607 | Log in to see | Polyclonal | 0 | |||
| 9.970049 | ABIN2430325 | IHC ELISA | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
| 4 | ABIN5928226 | ELISA IHC WB | Goat | Log in to see | Polyclonal | 0 | ||||
| 1 | ABIN1450332 | IHC ELISA | Rabbit | IgG | AA 478-527, pTyr512 | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN2292603 | IHC | Goat | AA 596-607 | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN2885529 | ELISA IHC | Rabbit | IgG | pTyr512 | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN2746783 | ELISA IHC IP WB | FITC | Rabbit | IgG | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN2746782 | ELISA IHC IP WB | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN6101383 | ELISA IHC WB | Rabbit | IgG | pTyr512 | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN6039675 | ELISA IHC | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN5558750 | ELISA IHC IP WB | Biotin | Rabbit | Log in to see | Polyclonal | 0 |
General |
|
|---|---|
| Antigen | IL2-Inducible T-Cell Kinase (ITK) Antibodies |
| Epitope | pTyr512 Alternatives |
| Reactivity | Human, Mouse (Murine) Alternatives |
| Host | Rabbit Alternatives |
| Clonality | |
| Conjugate | This ITK antibody is un-conjugated Alternatives |
| Application |
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Alternatives
|
| Supplier | Log in to see |
Product Details anti-ITK AntibodyTarget Details ITK Application Details Handling Images |
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| Specificity | ITK (Phospho-Tyr512) Antibody detects endogenous levels of ITK only when phosphorylated at Tyr512 |
| Cross-Reactivity | Human, Mouse (Murine) |
| Purification | The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns. |
| Immunogen | A synthesized peptide derived from human ITK (Phospho-Tyr512) |
| Isotype | IgG |
| Plasmids, Primers & others | |
Target Details ITKProduct Details anti-ITK Antibody Application Details Handling Images back to top |
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| Antigen | |
| Alternative Name | ITK (ITK Antibody Abstract) |
| Background |
Description: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation (PubMed:12186560, PubMed:12682224, PubMed:21725281). Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3 (By similarity). Gene: ITK |
| Molecular Weight | 68kDa |
| Gene ID | 3702 |
| UniProt | Q08881 |
| Pathways | TCR Signaling, Fc-epsilon Receptor Signaling Pathway |
Application DetailsProduct Details anti-ITK Antibody Target Details ITK Handling Images back to top |
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| Application Notes | WB 1:1000-3000, IHC 1:50-1:200 |
| Restrictions | For Research Use only |
HandlingProduct Details anti-ITK Antibody Target Details ITK Application Details Images back to top |
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| Format | Liquid |
| Concentration | 1 mg/mL |
| Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol. |
| Preservative | Sodium azide |
| Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Storage | -20 °C |
| Storage Comment | Store at -20 °C.Stable for 12 months from date of receipt |
| Expiry Date | 12 months |
ImagesProduct Details anti-ITK Antibody Target Details ITK Application Details Handling back to top |
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| Supplier Images |
ABIN6271311 at 1/200 staining Human kidney tissue sections by IHC-P. The tissue was f...
ABIN6271311 at 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was f...
ABIN6271311 at 1/200 staining Rat spleen tissue sections by IHC-P. The tissue was for...
Western blot analysis ITK (Phospho-Tyr512) using UV treated 293 whole cell lysates
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