anti-Human ITK antibody for Immunohistochemistry

Recommended ITK Antibody (supplied by: Log in to see )

Antigen
IL2-Inducible T-Cell Kinase (ITK) Antibodies
  • Emt
  • Tcsk
  • Tsk
  • EMT
  • LPFS1
  • LYK
  • PSCTK2
  • IL2 inducible T cell kinase
  • IL2 inducible T-cell kinase
  • IL2-inducible T-cell kinase
  • Itk
  • ITK
Epitope
pTyr512
23
18
17
14
9
7
5
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
119
27
25
3
2
Host
Rabbit
76
35
23
Clonality
Polyclonal
Conjugate
This ITK antibody is un-conjugated
8
7
6
6
6
6
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
119
85
16
10
8
5
5
5
4
3
1
Supplier
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Catalog No. ABIN6271310
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
15.579585 ABIN1589997 ELISA IHC WB Goat Internal Region Log in to see Polyclonal 0
9.970049 ABIN609692 IHC IHC (p) Goat AA 596-607 Log in to see Polyclonal 0
9.970049 ABIN2430325 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
4 ABIN5928226 ELISA IHC WB Goat Log in to see Polyclonal 0
1 ABIN1450332 IHC ELISA Rabbit IgG AA 478-527, pTyr512 Log in to see Polyclonal 0
1 ABIN2292603 IHC Goat AA 596-607 Log in to see Polyclonal 0
1 ABIN2885529 ELISA IHC Rabbit IgG pTyr512 Log in to see Polyclonal 0
1 ABIN2746783 ELISA IHC IP WB FITC Rabbit IgG Log in to see Polyclonal 0
1 ABIN2746782 ELISA IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6101383 ELISA IHC WB Rabbit IgG pTyr512 Log in to see Polyclonal 0
1 ABIN6039675 ELISA IHC Rabbit IgG Log in to see Polyclonal 0
1 ABIN5558750 ELISA IHC IP WB Biotin Rabbit Log in to see Polyclonal 0

General

Antigen IL2-Inducible T-Cell Kinase (ITK) Antibodies
Epitope pTyr512
(23), (18), (17), (14), (9), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(119), (27), (25), (3), (2)
Host Rabbit
(76), (35), (23)
Clonality
Polyclonal
Conjugate This ITK antibody is un-conjugated
(8), (7), (6), (6), (6), (6)
Application ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(119), (85), (16), (10), (8), (5), (5), (5), (4), (3), (1)
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Product Details anti-ITK Antibody

Target Details ITK Application Details Handling Images
Specificity ITK (Phospho-Tyr512) Antibody detects endogenous levels of ITK only when phosphorylated at Tyr512
Cross-Reactivity Human, Mouse (Murine)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human ITK (Phospho-Tyr512)
Isotype IgG
Plasmids, Primers & others

Target Details ITK

Product Details anti-ITK Antibody Application Details Handling Images back to top
Antigen
Alternative Name ITK (ITK Antibody Abstract)
Background

Description: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation (PubMed:12186560, PubMed:12682224, PubMed:21725281). Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3 (By similarity).

Gene: ITK

Molecular Weight 68kDa
Gene ID 3702
UniProt Q08881
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-ITK Antibody Target Details ITK Handling Images back to top
Application Notes WB 1:1000-3000, IHC 1:50-1:200
Restrictions For Research Use only

Handling

Product Details anti-ITK Antibody Target Details ITK Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-ITK Antibody Target Details ITK Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-IL2-Inducible T-Cell Kinase (ITK) (pTyr512) antibody (ABIN6271310) ABIN6271311 at 1/200 staining Human kidney tissue sections by IHC-P. The tissue was f...
Immunohistochemistry (IHC) image for anti-IL2-Inducible T-Cell Kinase (ITK) (pTyr512) antibody (ABIN6271310) ABIN6271311 at 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was f...
Immunohistochemistry (IHC) image for anti-IL2-Inducible T-Cell Kinase (ITK) (pTyr512) antibody (ABIN6271310) ABIN6271311 at 1/200 staining Rat spleen tissue sections by IHC-P. The tissue was for...
Western Blotting (WB) image for anti-IL2-Inducible T-Cell Kinase (ITK) (pTyr512) antibody (ABIN6271310) Western blot analysis ITK (Phospho-Tyr512) using UV treated 293 whole cell lysates