anti-Human LAT antibody for ELISA

Recommended LAT Antibody (supplied by: Log in to see )

Antigen
Linker For Activation of T Cells (LAT) Antibodies
  • LAT
  • lat
  • LAT1
  • pp36
  • linker for activation of T-cells
  • linker for activation of T cells
  • LAT
  • lat
  • Lat
Epitope
AA 1-234
31
25
22
22
14
7
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
279
122
103
3
2
1
Host
Mouse
168
103
14
2
Clonality
Monoclonal
Conjugate
This LAT antibody is un-conjugated
9
9
8
7
7
6
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
215
81
52
50
39
33
32
10
6
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN393665
$ 527.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19 ABIN6271566 ELISA ICC IF IHC WB Rabbit IgG pTyr161 Log in to see Polyclonal 0
16.25679 ABIN185255 ELISA WB Goat C-Term Log in to see Polyclonal 1
16.25679 ABIN3185361 ELISA WB Rabbit IgG Tyr659 Log in to see Polyclonal 0
16.25679 ABIN3185362 ELISA WB Rabbit IgG Tyr660 Log in to see Polyclonal 0
16.25679 ABIN3188076 ELISA WB Rabbit IgG Log in to see Polyclonal 0
14.149809 ABIN565313 IHC (p) ELISA WB Mouse IgG1 kappa AA 1-233 Log in to see 3B11-1D5 0
13.25679 ABIN3185360 ELISA WB Rabbit IgG Tyr657 Log in to see Polyclonal 0
13.25679 ABIN3182545 ELISA IHC WB Rabbit IgG pTyr191 Log in to see Polyclonal 0
13.25679 ABIN3182224 ELISA WB Rabbit IgG pTyr200 Log in to see Polyclonal 0
13.25679 ABIN5618163 ELISA IHC WB Rabbit IgG pTyr191 Log in to see Polyclonal 0
13.25679 ABIN5618165 ELISA WB Rabbit IgG Tyr191 Log in to see Polyclonal 0
13.25679 ABIN5618164 ELISA WB Rabbit IgG Tyr171 Log in to see Polyclonal 0
13.25679 ABIN2141365 ELISA WB Rabbit IgG Tyr161 Log in to see Polyclonal 0
13.25679 ABIN2145609 ELISA WB Rabbit IgG Log in to see Polyclonal 0
13.25679 ABIN2141089 ELISA WB Rabbit IgG pTyr171 Log in to see Polyclonal 0
13.25679 ABIN2141370 ELISA WB Rabbit IgG Tyr255 Log in to see Polyclonal 0
13 ABIN6269667 ELISA IHC WB Rabbit IgG pTyr45 Log in to see Polyclonal 0
10 ABIN6272038 ELISA ICC IF WB Rabbit IgG pTyr255 Log in to see Polyclonal 0
7 ABIN1531336 IHC ELISA WB Rabbit IgG AA 191-240, pTyr191 Log in to see Polyclonal 0
7 ABIN6269669 ELISA IHC WB Rabbit IgG pSer84 Log in to see Polyclonal 0

General

Antigen Linker For Activation of T Cells (LAT) Antibodies
Epitope AA 1-234
(31), (25), (22), (22), (14), (7), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(279), (122), (103), (3), (2), (1)
Host Mouse
(168), (103), (14), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This LAT antibody is un-conjugated
(9), (9), (8), (7), (7), (6), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1)
Application ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(215), (81), (52), (50), (39), (33), (32), (10), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-LAT Antibody

Target Details LAT Application Details Handling References for anti-LAT antibody (ABIN393665) Images
Specificity LAT (AAH11563, 1 a.a. ~ 234 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: LAT (AAH11563, 1 a.a. ~ 233 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 3B11-1D5
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details LAT

Product Details anti-LAT Antibody Application Details Handling References for anti-LAT antibody (ABIN393665) Images back to top
Antigen
Alternative Name LAT (LAT Antibody Abstract)
Background

Other Names: Linker for activation of T-cells family member 1, 36 kDa phospho-tyrosine adapter protein, pp36, p36-38, LAT

Gene Description: linker for activation of T cells

Gene Summary: The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq

Molecular Weight NONE?! DA
Gene ID 27040
NCBI Accession NP_001014987, NP_001014988, NP_001014989, NP_055202
UniProt O43561
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-LAT Antibody Target Details LAT Handling References for anti-LAT antibody (ABIN393665) Images back to top
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.

Restrictions For Research Use only

Handling

Product Details anti-LAT Antibody Target Details LAT Application Details References for anti-LAT antibody (ABIN393665) Images back to top
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-LAT antibody (ABIN393665)

Product Details anti-LAT Antibody Target Details LAT Application Details Handling Images back to top
Background publications

Davila, Froeling, Tan, Bonnard, Boland, Snippe, Hibberd, Seielstad: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010

Sowa, Bennett, Gygi, Harper: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009

Cruz-Orcutt, Houtman: "PI3 kinase function is vital for the function but not formation of LAT-mediated signaling complexes." in: Molecular immunology, Vol. 46, Issue 11-12, pp. 2274-83, 2009

Shen, Zhu, Lau, Chuck, Zhang: "The essential role of LAT in thymocyte development during transition from the double-positive to single-positive stage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 9, pp. 5596-604, 2009

Saito, Kawamoto, Kamatani: "Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects." in: Journal of human genetics, Vol. 54, Issue 6, pp. 317-23, 2009

Images

Product Details anti-LAT Antibody Target Details LAT Application Details Handling References for anti-LAT antibody (ABIN393665) back to top
Supplier Images
Western Blotting (WB) image for anti-Linker For Activation of T Cells (LAT) (AA 1-234) antibody (ABIN393665) LAT monoclonal antibody (M01), clone 3B11-1D5 Western Blot analysis of LAT expression...
Immunohistochemistry (IHC) image for anti-Linker For Activation of T Cells (LAT) (AA 1-234) antibody (ABIN393665) anti-Linker For Activation of T Cells (LAT) (AA 1-234) antibody (Image 2)
Western Blotting (WB) image for anti-Linker For Activation of T Cells (LAT) (AA 1-234) antibody (ABIN393665) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Immunohistochemistry (IHC) image for anti-Linker For Activation of T Cells (LAT) (AA 1-234) antibody (ABIN393665) Immunoperoxidase of monoclonal antibody to LAT on formalin-fixed paraffin-embedded hu...
ELISA image for anti-Linker For Activation of T Cells (LAT) (AA 1-234) antibody (ABIN393665) Detection limit for recombinant GST tagged LAT is approximately 0.03ng/ml as a captur...