anti-Rat (Rattus) RIPK2 antibody for Immunocytochemistry

Recommended RIPK2 Antibody (supplied by: Log in to see )

Antigen
Receptor-Interacting Serine-threonine Kinase 2 (RIPK2) Antibodies
  • RIPK2
  • 2210420D18Rik
  • CARD3
  • CARDIAK
  • CCK
  • D4Bwg0615e
  • RICK
  • RIP2
  • GIG30
  • receptor interacting serine/threonine kinase 2
  • receptor (TNFRSF)-interacting serine-threonine kinase 2
  • receptor-interacting serine-threonine kinase 2
  • RIPK2
  • Ripk2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
153
68
34
6
5
4
4
3
3
2
1
Host
Rabbit
137
14
6
Clonality
Polyclonal
Conjugate
This RIPK2 antibody is un-conjugated
6
6
4
2
2
2
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
148
84
66
23
22
21
7
4
3
3
2
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Catalog No. ABIN6264766
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN6258316 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN201933 ICC WB Rabbit IgG N-Term Log in to see Polyclonal 0
7 ABIN1031540 ICC ELISA WB Rabbit N-Term Log in to see Polyclonal 0
7 ABIN5014250 ICC IHC WB Rabbit IgG AA 1-294 Log in to see Polyclonal 0
1 ABIN1003093 ICC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 3
1 ABIN1104002 ICC WB Rabbit IgG AA 11-30 Log in to see Polyclonal 0
1 ABIN1888198 ICC ELISA WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN2959906 ICC ELISA WB Rabbit N-Term Log in to see Polyclonal 0

General

Antigen Receptor-Interacting Serine-threonine Kinase 2 (RIPK2) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(153), (68), (34), (6), (5), (4), (4), (3), (3), (2), (1)
Host Rabbit
(137), (14), (6)
Clonality
Polyclonal
Conjugate This RIPK2 antibody is un-conjugated
(6), (6), (4), (2), (2), (2)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(148), (84), (66), (23), (22), (21), (7), (4), (3), (3), (2)
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Product Details anti-RIPK2 Antibody

Target Details RIPK2 Application Details Handling Images
Specificity RIPK2 Antibody detects endogenous levels of total RIPK2
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthesized peptide derived from human RIPK2
Isotype IgG
Plasmids, Primers & others

Target Details RIPK2

Product Details anti-RIPK2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name RIPK2 (RIPK2 Antibody Abstract)
Background

Description: Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

Gene: RIPK2

Molecular Weight 61kDa
Gene ID 8767
UniProt O43353
Pathways TCR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades

Application Details

Product Details anti-RIPK2 Antibody Target Details RIPK2 Handling Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-RIPK2 Antibody Target Details RIPK2 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-RIPK2 Antibody Target Details RIPK2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Receptor-Interacting Serine-threonine Kinase 2 (RIPK2) antibody (ABIN6264766) Western blot analysis of K562 whole cell lysates, using RIPK2 Antibody. The lane on t...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Receptor-Interacting Serine-threonine Kinase 2 (RIPK2) antibody (ABIN6264766) ABIN6277226 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabi...
Immunohistochemistry (IHC) image for anti-Receptor-Interacting Serine-threonine Kinase 2 (RIPK2) antibody (ABIN6264766) ABIN6277226 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was form...