anti-Rat (Rattus) UBE2N antibody for Western Blotting

Recommended UBE2N Antibody (supplied by: Log in to see )

Antigen
Ubiquitin-Conjugating Enzyme E2N (UBE2N) Antibodies
  • UBC13
  • UbcH-ben
  • UbcH13
  • 1500026J17Rik
  • AL022654
  • BB101821
  • ube2n
  • wu:fb11b03
  • wu:fc08b06
  • zgc:55726
  • ubc13
  • ubch-ben
  • UBE2N
  • ubiquitin conjugating enzyme E2 N
  • ubiquitin-conjugating enzyme E2N
  • ubiquitin conjugating enzyme E2 N S homeolog
  • ubiquitin-conjugating enzyme E2Na
  • UBE2N
  • Ube2n
  • ube2n.S
  • ube2na
  • LOC100533434
  • ube2n
Epitope
Middle Region
16
8
5
4
3
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Yeast (Saccharomyces cerevisiae), Zebrafish (Danio rerio)
87
51
48
10
9
6
6
5
4
3
3
3
3
3
2
2
1
1
1
1
1
1
Host
Rabbit
70
9
8
Clonality
Polyclonal
Conjugate
This UBE2N antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
71
34
20
18
15
13
5
5
3
1
1
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Catalog No. ABIN2778258
$ 229.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1603587 ABIN2777765 IHC WB Rabbit Middle Region Log in to see Polyclonal 1
3.1603587 ABIN4905540 WB Rabbit IgG Log in to see Polyclonal 0
3.1603587 ABIN2460659 ELISA WB Rabbit Log in to see Polyclonal 0
3.1603587 ABIN2460860 ELISA WB Rabbit Log in to see Polyclonal 0
3.1603587 ABIN2477023 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 3
3.1603587 ABIN5966007 WB Rabbit IgG Log in to see Polyclonal 0
3.1603587 ABIN1030789 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 0
3.1603587 ABIN629720 WB Rabbit Log in to see Polyclonal 0
3.1603587 ABIN629730 WB Rabbit Log in to see Polyclonal 0
3.1603587 ABIN5999134 WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN265157 IHC (fro) WB Rabbit IgG C-Term Log in to see Polyclonal 6
1 ABIN5551720 EIA WB Goat IgG AA 40-51 Log in to see Polyclonal 0
1 ABIN324309 WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN202033 WB Rabbit IgG AA 80-129 Log in to see Polyclonal 0
1 ABIN214016 IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN5555405 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN314970 ICC IF IHC (p) IHC ELISA WB Goat Log in to see Polyclonal 0
1 ABIN303157 EIA IF IHC (p) WB Goat AA 40-51 Log in to see Polyclonal 0
1 ABIN1387434 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1740534 IHC (p) WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Ubiquitin-Conjugating Enzyme E2N (UBE2N) Antibodies
Epitope Middle Region
(16), (8), (5), (4), (3), (2), (2), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Yeast (Saccharomyces cerevisiae), Zebrafish (Danio rerio)
(87), (51), (48), (10), (9), (6), (6), (5), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(70), (9), (8)
Clonality
Polyclonal
Conjugate This UBE2N antibody is un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(71), (34), (20), (18), (15), (13), (5), (5), (3), (1), (1)
Pubmed 2 references available
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Product Details anti-UBE2N Antibody

Target Details UBE2N Application Details Handling References for anti-UBE2N antibody (ABIN2778258) Images
Sequence VDKLGRICLD ILKDKWSPAL QIRTVLLSIQ ALLSAPNPDD PLANDVAEQW
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Yeast: 100%, Zebrafish: 100%
Characteristics This is a rabbit polyclonal antibody against UBE2N. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human UBE2N
Plasmids, Primers & others

Target Details UBE2N

Product Details anti-UBE2N Antibody Application Details Handling References for anti-UBE2N antibody (ABIN2778258) Images back to top
Antigen
Alternative Name UBE2N (UBE2N Antibody Abstract)
Background UBE2N encodes a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
Alias Symbols: UBC13, UbcH-ben
Protein Interaction Partner: TRIM33, BFAR, MALT1, STUB1, BCL10, UBE2V2, UBC, TRAF6, ZNRF1, XIAP, SLX8, SLX5, MARCH7, TNF, UBE2N, RNF168, RNF8, PARK2, OTUB1, ARIH1, NEDD4L, MFN2, TRIM72, PELI3, RNF111, RNF11, BAG3, TRAF2, FN1, VCAM1, RNF126, RNF115, NBN, ATP6V1H, VPS33B, ZDHHC5, ACTR2
Protein Size: 152
Molecular Weight 17 kDa
Gene ID 7334
NCBI Accession NM_003348, NP_003339
UniProt P61088
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus

Application Details

Product Details anti-UBE2N Antibody Target Details UBE2N Handling References for anti-UBE2N antibody (ABIN2778258) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 152 AA

Restrictions For Research Use only

Handling

Product Details anti-UBE2N Antibody Target Details UBE2N Application Details References for anti-UBE2N antibody (ABIN2778258) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-UBE2N antibody (ABIN2778258)

Product Details anti-UBE2N Antibody Target Details UBE2N Application Details Handling Images back to top
Product cited in:

Xu, Yan, Jovanovic, An, Cheng, Aakre, Yi, Eng, Link, Moses: "Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts." in: Proteomics, Vol. 10, Issue 13, pp. 2458-70, 2010

Background publications

Bothos, Summers, Venere, Scolnick, Halazonetis: "The Chfr mitotic checkpoint protein functions with Ubc13-Mms2 to form Lys63-linked polyubiquitin chains." in: Oncogene, Vol. 22, Issue 46, pp. 7101-7, 2003

Images

Product Details anti-UBE2N Antibody Target Details UBE2N Application Details Handling References for anti-UBE2N antibody (ABIN2778258) back to top
Supplier Images
Western Blotting (WB) image for anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) (Middle Region) antibody (ABIN2778258) WB Suggested Anti-UBE2N Antibody Titration: 1.25ug/ml Positive Control: HepG2 cell...
Western Blotting (WB) image for anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) (Middle Region) antibody (ABIN2778258) WB Suggested Anti-UBE2N Antibody Titration: 1.25 µg/mL Positive Control: HepG2 cell...
Western Blotting (WB) image for anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) (Middle Region) antibody (ABIN2778258) Lanes: 1: 40ng HIS-UBE2D1 protein 2: 40ng HIS-UBE2D2 protein 3: 40ng HIS-UBE2D3 pro...
Immunohistochemistry (IHC) image for anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) (Middle Region) antibody (ABIN2778258) Sample Type : Human brain stem cells Primary Antibody Dilution : 1:500 Secondary ...