anti-Human VAV1 antibody for Immunofluorescence

Recommended VAV1 Antibody (supplied by: Log in to see )

Antigen
Vav 1 Oncogene (VAV1) Antibodies
  • VAV
  • Vav
  • vav-T
  • vav guanine nucleotide exchange factor 1
  • vav 1 oncogene
  • vav guanine nucleotide exchange factor 1 L homeolog
  • VAV1
  • Vav1
  • vav1.L
Epitope
AA 681-791
36
25
25
15
10
9
8
7
6
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
178
84
83
4
4
2
2
2
2
1
Host
Mouse
146
34
Clonality
Monoclonal
Conjugate
This VAV1 antibody is un-conjugated
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunofluorescence (IF), Western Blotting (WB)
155
72
34
14
14
12
8
8
6
2
1
1
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Catalog No. ABIN394203
$ 461.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
20.313152 ABIN394445 IF WB Mouse IgG2b kappa AA 681-791 Log in to see 9C1 5
17.313152 ABIN563363 IF ELISA WB Mouse IgG2b kappa AA 681-790, partial Log in to see 9C1 0
17.313152 ABIN521299 IF ELISA WB Mouse IgG2a kappa AA 681-790, partial Log in to see 3A11 0
13 ABIN6256653 ELISA ICC IF IHC WB Rabbit IgG pTyr174 Log in to see Polyclonal 0
13 ABIN6271208 ELISA ICC IF IHC WB Rabbit IgG pTyr160 Log in to see Polyclonal 0
13 ABIN6265950 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
12.576219 ABIN5621472 ELISA IF Rabbit IgG total Log in to see Polyclonal 0
8.313151 ABIN1532426 IF ELISA Rabbit IgG AA 131-180 Log in to see Polyclonal 1
7 ABIN5590685 IF IHC (p) Rabbit IgG Log in to see Polyclonal 0
4 ABIN2888713 IF ELISA Rabbit IgG AA 131-180 Log in to see Polyclonal 0
4 ABIN5957822 ELISA IF WB Rabbit IgG Tyr160 Log in to see Polyclonal 0
1 ABIN1829109 IF ELISA WB Mouse IgG2b, kappa AA 681-790 Log in to see 9C1 0
1 ABIN2384822 IF ELISA WB Mouse IgG2a kappa AA 681-790 Log in to see 3A11 0

General

Antigen Vav 1 Oncogene (VAV1) Antibodies
Epitope AA 681-791
(36), (25), (25), (15), (10), (9), (8), (7), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(178), (84), (83), (4), (4), (2), (2), (2), (2), (1)
Host Mouse
(146), (34)
Clonality (Clone)
Monoclonal   ( )
Conjugate This VAV1 antibody is un-conjugated
(4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunofluorescence (IF), Western Blotting (WB)
(155), (72), (34), (14), (14), (12), (8), (8), (6), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-VAV1 Antibody

Target Details VAV1 Application Details Handling References for anti-VAV1 antibody (ABIN394203) Images
Specificity VAV1 (AAH13361, 681 a.a. ~ 791 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: VAV1 (AAH13361, 681 a.a. ~ 790 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 3A11
Isotype IgG2a kappa
Plasmids, Primers & others

Target Details VAV1

Product Details anti-VAV1 Antibody Application Details Handling References for anti-VAV1 antibody (ABIN394203) Images back to top
Antigen
Alternative Name VAV1 (VAV1 Antibody Abstract)
Background

Other Names: Proto-oncogene vav, VAV1, VAV

Gene Description: vav 1 guanine nucleotide exchange factor

Gene Summary: The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. [provided by RefSeq

Molecular Weight 98314 DA
Gene ID 7409
NCBI Accession NP_001245136, NP_005419
UniProt P15498
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events

Application Details

Product Details anti-VAV1 Antibody Target Details VAV1 Handling References for anti-VAV1 antibody (ABIN394203) Images back to top
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication.

Restrictions For Research Use only

Handling

Product Details anti-VAV1 Antibody Target Details VAV1 Application Details References for anti-VAV1 antibody (ABIN394203) Images back to top
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-VAV1 antibody (ABIN394203)

Product Details anti-VAV1 Antibody Target Details VAV1 Application Details Handling Images back to top
Background publications

Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010

Barda-Saad, Shirasu, Pauker, Hassan, Perl, Balbo, Yamaguchi, Houtman, Appella, Schuck, Samelson: "Cooperative interactions at the SLP-76 complex are critical for actin polymerization." in: The EMBO journal, Vol. 29, Issue 14, pp. 2315-28, 2010

Davila, Froeling, Tan, Bonnard, Boland, Snippe, Hibberd, Seielstad: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010

Kim, Das, Gross, Bryceson, Long: "Synergistic signals for natural cytotoxicity are required to overcome inhibition by c-Cbl ubiquitin ligase." in: Immunity, Vol. 32, Issue 2, pp. 175-86, 2010

Yu, Martins, Li, Amarasinghe, Umetani, Fernandez-Zapico, Billadeau, Machius, Tomchick, Rosen: "Structural and energetic mechanisms of cooperative autoinhibition and activation of Vav1." in: Cell, Vol. 140, Issue 2, pp. 246-56, 2010

Images

Product Details anti-VAV1 Antibody Target Details VAV1 Application Details Handling References for anti-VAV1 antibody (ABIN394203) back to top
Supplier Images
Western Blotting (WB) image for anti-Vav 1 Oncogene (VAV1) (AA 681-791) antibody (ABIN394203) Immunofluorescence of monoclonal antibody to VAV1 on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-Vav 1 Oncogene (VAV1) (AA 681-791) antibody (ABIN394203) anti-Vav 1 Oncogene (VAV1) (AA 681-791) antibody (Image 2)
Western Blotting (WB) image for anti-Vav 1 Oncogene (VAV1) (AA 681-791) antibody (ABIN394203) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Western Blotting (WB) image for anti-Vav 1 Oncogene (VAV1) (AA 681-791) antibody (ABIN394203) VAV1 monoclonal antibody (M04), clone 3A11 Western Blot analysis of VAV1 expression i...
Immunofluorescence (IF) image for anti-Vav 1 Oncogene (VAV1) (AA 681-791) antibody (ABIN394203) Immunofluorescence of monoclonal antibody to VAV1 on HeLa cell. [antibody concentrati...
ELISA image for anti-Vav 1 Oncogene (VAV1) (AA 681-791) antibody (ABIN394203) Detection limit for recombinant GST tagged VAV1 is approximately 1ng/ml as a capture ...