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|Antigen||Vimentin (VIM) Antibodies|
|Reactivity||Chicken, Cow (Bovine), Hamster, Human, Mouse (Murine), Xenopus laevis Alternatives|
|Host||Guinea Pig Alternatives|
|Conjugate||This Vimentin antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
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Product Details anti-Vimentin AntibodyTarget Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN126094) Images
|Specificity||Specific detection of Vimentin (Mr 57,000 polypeptide). Tumors specifically detected: Sarcoma (including myosarcoma), lymphoma, melanoma.|
Species reactivity (expected):Hamster, Chicken, Xenopus.
Species reactivity (tested):Human, Bovine, Mouse.
|Immunogen||Vimentin purified from calf lens|
Target Details VimentinProduct Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN126094) Images back to top
|Alternative Name||Vimentin (VIM Antibody Abstract)|
|Background||Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.Synonyms: VIM|
|Research Area||Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers|
|Pathways||Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN126094) Images back to top
Immunohistochemistry on frozen sections: 1/100. Immunohistochemistry on paraffin sections: 1/50, 1h at RT. Immunoblotting: 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN126094) Images back to top
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
References for anti-Vimentin antibody (ABIN126094)Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Boehnke, Mirancea, Pavesio, Fusenig, Boukamp, Stark: "Effects of fibroblasts and microenvironment on epidermal regeneration and tissue function in long-term skin equivalents." in: European journal of cell biology, Vol. 86, Issue 11-12, pp. 731-46, 2007
Magin, Bader, Freudenmann, Franke: "De novo formation of cytokeratin filaments in calf lens cells and cytoplasts after transfection with cDNAs or microinjection with mRNAs encoding human cytokeratins." in: European journal of cell biology, Vol. 53, Issue 2, pp. 333-48, 1991