anti-Human ZAP70 antibody for Immunocytochemistry

Recommended ZAP70 Antibody

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • SRK
  • STCD
  • STD
  • TZK
  • ZAP-70
  • 70kDa
  • AI327364
  • Srk
  • mrtle
  • mur
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • zgc:110383
  • zeta chain of T-cell receptor associated protein kinase 70
  • putative tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta chain of T cell receptor associated protein kinase 70
  • zeta-chain (TCR) associated protein kinase 70kDa
  • zeta chain of T cell receptor associated protein kinase 70kDa L homeolog
  • zeta chain of T cell receptor associated protein kinase 70kDa
  • tyrosine-protein kinase ZAP-70
  • ZAP70
  • Smp_149470
  • Zap70
  • zap70.L
  • zap70
  • LOC707555
  • LOC100342021
  • LOC100390922
  • LOC100542877
Epitope
C-Term
60
57
54
30
29
26
23
14
12
12
11
11
10
7
7
7
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
619
259
184
14
11
9
8
7
6
6
4
3
1
Host
Mouse
387
214
17
3
2
Clonality
Monoclonal
Conjugate
This ZAP70 antibody is un-conjugated
29
29
18
18
14
12
10
10
10
10
10
10
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)
337
200
188
166
120
90
65
52
43
12
8
7
3
2
2
2
Options

Available images

Catalog No. ABIN125756
$ 377.37
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
19 ABIN6255884 ELISA ICC IF IHC WB Rabbit IgG pTyr319 Polyclonal 0
16 ABIN1875382 ICC IP IHC WB Rabbit IgG Polyclonal 0
13 ABIN6256749 ELISA ICC IF IHC WB Rabbit IgG pTyr493 Polyclonal 0
10 ABIN6266086 ELISA ICC IF IHC WB Rabbit IgG Polyclonal 0
10 ABIN6266088 ELISA ICC IF IHC WB Rabbit IgG Polyclonal 0
7 ABIN6266087 ELISA ICC IF IHC WB Rabbit IgG Polyclonal 0
1 ABIN207127 Inhibition FACS ICC WB Mouse IgG1 C-Term ZAP-03 0
1 ABIN210659 ICC IP WB Mouse IgG1 AA 282-307 0
1 ABIN198253 ICC FITC Mouse IgG2a AA 1-254 0
1 ABIN221118 ICC FACS IF IP WB Rabbit IgG pTyr319 Polyclonal 0
1 ABIN6758909 ICC IHC IP WB Rabbit IgG Polyclonal 0
1 ABIN3200826 FACS ICC WB Mouse IgG1 C-Term ZAP-03 0

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope C-Term
(60), (57), (54), (30), (29), (26), (23), (14), (12), (12), (11), (11), (10), (7), (7), (7), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(619), (259), (184), (14), (11), (9), (8), (7), (6), (6), (4), (3), (1)
Host Mouse
(387), (214), (17), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ZAP70 antibody is un-conjugated
(29), (29), (18), (18), (14), (12), (10), (10), (10), (10), (10), (10), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)
(337), (200), (188), (166), (120), (90), (65), (52), (43), (12), (8), (7), (3), (2), (2), (2)
Pubmed 3 references available
Supplier

Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling References for anti-ZAP70 antibody (ABIN125756) Images
Specificity The antibody ZAP-03 reacts with ZAP-70, a 70 kDa protein tyrosine kinase expressed in T and NK cells.
ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Immunogen Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP-70 with histidine tag
Clone ZAP-03
Isotype IgG1
Plasmids, Primers & others

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling References for anti-ZAP70 antibody (ABIN125756) Images back to top
Antigen
Alternative Name ZAP-70 (ZAP70 Antibody Abstract)
Background The ZAP-70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP-70 gene mutations resulting in lower ZAP-70 protein expression levels or expression of catalytically inactive ZAP-70 proteins, have been identified. ZAP-70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency.In patients with chronic lymphocytic leukemia (B-CLL), ZAP-70 expression on B cell was shown to be correlated with disease progression and survival. ZAP-70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP-70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP-70 on Tyr493 in the activation loop. ZAP-70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP-70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling References for anti-ZAP70 antibody (ABIN125756) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details References for anti-ZAP70 antibody (ABIN125756) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-ZAP70 antibody (ABIN125756)

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling Images back to top
Product cited in:

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, 2011

Background publications

Schneider, Smith, Liu, Bismuth, Rudd: "CTLA-4 disrupts ZAP70 microcluster formation with reduced T cell/APC dwell times and calcium mobilization." in: European journal of immunology, Vol. 38, Issue 1, pp. 40-7, 2007

Ishaq, DeGray, Natarajan: "Evidence for the involvement of tyrosine kinase ZAP 70 in nuclear retinoid receptor-dependent transactivation in T lymphocytes." in: The Journal of biological chemistry, Vol. 280, Issue 40, pp. 34152-8, 2005

Images

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling References for anti-ZAP70 antibody (ABIN125756) back to top
Supplier Images
Western Blotting (WB) image for anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (C-Term) antibody (ABIN125756) Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell le...
Western Blotting (WB) image for anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (C-Term) antibody (ABIN125756) Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell le...