anti-Human ZAP70 antibody for Immunocytochemistry

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • SRK
  • STCD
  • STD
  • TZK
  • ZAP-70
  • 70kDa
  • AI327364
  • Srk
  • mrtle
  • mur
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • zgc:110383
  • zeta chain of T-cell receptor associated protein kinase 70
  • putative tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta chain of T cell receptor associated protein kinase 70
  • zeta-chain (TCR) associated protein kinase 70kDa
  • zeta chain of T cell receptor associated protein kinase 70kDa L homeolog
  • zeta chain of T cell receptor associated protein kinase 70kDa
  • tyrosine-protein kinase ZAP-70
  • ZAP70
  • Smp_149470
  • Zap70
  • zap70.L
  • zap70
  • LOC707555
  • LOC100342021
  • LOC100390922
  • LOC100542877
Epitope
pTyr493
101
51
51
34
31
25
24
14
12
12
11
11
10
7
7
7
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
641
224
146
14
11
10
8
7
6
6
4
3
1
Host
Rabbit
338
285
17
3
2
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
33
27
25
21
15
14
7
7
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
337
217
205
203
140
137
65
52
50
16
8
7
4
4
3
2
2
2
2
Supplier
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Catalog No. ABIN6256749
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6255884 ELISA ICC IF IHC WB Rabbit IgG pTyr319 Log in to see Polyclonal 0
1 ABIN1875382 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6266086 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6266087 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6266088 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN125756 ICC FACS WB Mouse IgG1 C-Term Log in to see ZAP-03 3
1 ABIN4366555 CyTOF FACS ICC IF IP WB Mouse IgG1 Log in to see 1E7-2 0
1 ABIN207127 Inhibition FACS ICC WB Mouse IgG1 C-Term Log in to see ZAP-03 0
1 ABIN210659 ICC IP WB Mouse IgG1 AA 282-307 Log in to see 0
1 ABIN198253 ICC FITC Mouse IgG2a AA 1-254 Log in to see 0
1 ABIN221118 ICC FACS IF IP WB Rabbit IgG pTyr319 Log in to see Polyclonal 0
1 ABIN2880178 ICC IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5591107 FACS ICC IP WB FITC Mouse IgG1 Log in to see 1E7-2 0
1 ABIN5591108 FACS ICC IP WB PE Mouse IgG1 Log in to see 1E7-2 0
1 ABIN3200826 FACS ICC WB Mouse IgG1 C-Term Log in to see ZAP-03 0
1 ABIN5591106 FACS ICC IP WB Mouse IgG1 Log in to see 1E7-2 0

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope pTyr493
(101), (51), (51), (34), (31), (25), (24), (14), (12), (12), (11), (11), (10), (7), (7), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(641), (224), (146), (14), (11), (10), (8), (7), (6), (6), (4), (3), (1)
Host Rabbit
(338), (285), (17), (3), (2)
Clonality
Polyclonal
Conjugate This ZAP70 antibody is un-conjugated
(33), (27), (25), (21), (15), (14), (7), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(337), (217), (205), (203), (140), (137), (65), (52), (50), (16), (8), (7), (4), (4), (3), (2), (2), (2), (2)
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling Images
Specificity Phospho-ZAP-70 (Tyr493) Antibody detects endogenous levels of ZAP-70 only when phosphorylated at Tyrosine 493
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human ZAP-70 around the phosphorylation site of Tyrosine 493
Isotype IgG
Plasmids, Primers & others

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ZAP-70 (ZAP70 Antibody Abstract)
Background

Description: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).

Gene: ZAP70

Molecular Weight 70kDa
Gene ID 7535
UniProt P43403
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling back to top
Supplier Images
Immunofluorescence (fixed cells) (IF/ICC) image for anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody (ABIN6256749) ABIN6267522 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeab...
Immunohistochemistry (IHC) image for anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody (ABIN6256749) ABIN6267522 at 1/200 staining human kidney tissue sections by IHC-P. The tissue was f...
Western Blotting (WB) image for anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody (ABIN6256749) Western blot analysis of Phospho-ZAP-70 (Tyr493) expression in various lysates
Western Blotting (WB) image for anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody (ABIN6256749) Western blot analysis of ZAP-70 phosphorylation expression in Jurkat whole cell lysat...