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|Antigen||zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This ZAP70 antibody is un-conjugated Alternatives|
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-ZAP70 AntibodyTarget Details ZAP70 Application Details Handling References for anti-ZAP70 antibody (ABIN967585) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
Target Details ZAP70Product Details anti-ZAP70 Antibody Application Details Handling References for anti-ZAP70 antibody (ABIN967585) Images back to top
|Alternative Name||ZAP-70 (ZAP70 Antibody Abstract)|
|Background||The 70-kDa zeta chain-associated protein (ZAP70) is a protein tyrosine kinase (PTK) that associates with the zeta subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both ZAP70 and Syk are recruited to the phosphorylated CD3 and zeta subunits after TCR stimulation. TCR-mediated Lck activity leads to phosphorylation of ZAP70 on Tyrosine 493 (Y493) in the regulatory loop of the kinase domain leading to upregulation of ZAP70 kinase activity. The significance of ZAP70 activation in mediating TCR signal transduction has been confirmed by showing that ZAP70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP70 kinase domain which affect the stability of the protein and TCR signaling. The 1a/Zap70 (pY493) antibody recognizes ZAP70 phosphorylated at Y493.|
|Molecular Weight||70 kDa|
|Research Area||Signaling, Tags/Labels, Immunology, Cancer|
Application DetailsProduct Details anti-ZAP70 Antibody Target Details ZAP70 Handling References for anti-ZAP70 antibody (ABIN967585) Images back to top
Related Products: ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-ZAP70 Antibody Target Details ZAP70 Application Details References for anti-ZAP70 antibody (ABIN967585) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-ZAP70 antibody (ABIN967585)Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling Images back to top
|Product cited in:||
Mège, Di Bartolo, Germain, Tuosto, Michel, Acuto: "Mutation of tyrosines 492/493 in the kinase domain of ZAP-70 affects multiple T-cell receptor signaling pathways." in: The Journal of biological chemistry, Vol. 271, Issue 51, pp. 32644-52, 1997
Chan, Dalton, Johnson, Kong, Wang, Thoma, Kurosaki: "Activation of ZAP-70 kinase activity by phosphorylation of tyrosine 493 is required for lymphocyte antigen receptor function." in: The EMBO journal, Vol. 14, Issue 11, pp. 2499-508, 1995
Chan, Kadlecek, Elder, Filipovich, Kuo, Iwashima, Parslow, Weiss: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." in: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, 1994
ImagesProduct Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling References for anti-ZAP70 antibody (ABIN967585) back to top