anti-Human ZAP70 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta chain of T cell receptor associated protein kinase 70kDa L homeolog
  • zeta chain of T cell receptor associated protein kinase 70kDa
  • zeta chain of T cell receptor associated protein kinase 70
  • tyrosine-protein kinase ZAP-70
  • putative tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase 70kDa
  • zeta-chain (TCR) associated protein kinase
  • zeta chain of T-cell receptor associated protein kinase 70
  • zap70.L
  • zap70
  • ZAP70
  • LOC707555
  • Smp_149470
  • LOC100342021
  • LOC100390922
  • LOC100542877
  • Zap70
Epitope
AA 1-254
119
53
51
36
31
26
25
14
12
12
11
11
10
7
7
7
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
683
246
152
14
11
10
8
7
6
6
4
3
1
Host
Mouse
346
319
17
3
2
Clonality
Monoclonal
Conjugate
This ZAP70 antibody is conjugated to CF405M
34
29
26
23
16
14
9
9
7
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
357
246
216
208
163
153
64
52
50
27
8
7
4
4
3
2
2
2
2
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN6189272
$ 209.23
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6189280 FACS IF IHC (f) CF®555 Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189290 FACS IF IHC (f) CF®680R Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189310 FACS IF IHC (f) CF405M Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189316 FACS IF IHC (f) CF®647 Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189312 FACS IF IHC (f) CF®640R Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189320 FACS IF IHC (f) CF®660R Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189324 FACS IF IHC (f) CF®770 Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189332 FACS IF IHC (f) CF®594 Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189330 FACS IF IHC (f) CF®488A Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189284 FACS IF IHC (f) CF®568 Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189292 FACS IF IHC (f) CF®488A Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189270 FACS IF IHC (f) CF®405S Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189308 FACS IF IHC (f) CF®405S Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189326 FACS IF IHC (f) CF®680 Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189278 FACS IF IHC (f) CF®647 Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189314 FACS IF IHC (f) CF®543 Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189322 FACS IF IHC (f) CF®568 Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189318 FACS IF IHC (f) CF®555 Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189276 FACS IF IHC (f) CF®543 Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189282 FACS IF IHC (f) CF®660R Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope AA 1-254
(119), (53), (51), (36), (31), (26), (25), (14), (12), (12), (11), (11), (10), (7), (7), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(683), (246), (152), (14), (11), (10), (8), (7), (6), (6), (4), (3), (1)
Host Mouse
(346), (319), (17), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ZAP70 antibody is conjugated to CF405M
(34), (29), (26), (23), (16), (14), (9), (9), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
(357), (246), (216), (208), (163), (153), (64), (52), (50), (27), (8), (7), (4), (4), (3), (2), (2), (2), (2)
Supplier Log in to see

Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling Images
Purpose Mouse Monoclonal anti-ZAP70 (2F3.2), CF405M Conjugate
Characteristics ZAP70 is a 70 kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene.In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70 kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25 % of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70
Clone 2F3-2
Isotype IgG2a, kappa
Plasmids, Primers & others

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ZAP70 (ZAP70 Antibody Abstract)
Background Selective T cell defect, SRK, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70,TZK, Zeta chain associated protein kinase 70  kDa
Molecular Weight 70 kDa
Gene ID 7535, 234569
UniProt P43403
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling Images back to top
Application Notes Immunohistology formalin-fixed 0.5-1 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Immunofluorescence 0.5-1 μg/mL
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Optimal dilution for a specific application should be determined by user
Comment

Jurkat cells. Tonsil or lymph node.

Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light