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|Antigen||zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies|
|Conjugate||This ZAP70 antibody is un-conjugated Alternatives|
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-ZAP70 AntibodyTarget Details ZAP70 Application Details Handling Images
|Purpose||This antibody is designed for the specific localization of human ZAP-70 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections. It helps in the detection of CLL subtypes and studies of Severe Combined Immunodeficiency Syndrome.|
|Characteristics||ZAP-70 is an abbrevation for Zeta-chain-associated protein kinase 70 (70 is the molecular weight in kDa). The protein is a member of the protein-tyrosine kinase family. ZAP-70 is normally expressed in T-cells and natural killer cells and has a critical role in the initiation of T-cell signaling. ZAP-70 in B- cells is used as a prognostic marker in identifying different forms of Chronic Lymphocytic Leukemia (CLL). ZAP-70 protein is expressed in leukemic cells in approximately 25% of Chronic Lymphocytic Leukemia (CLL) cases as well. ZAP-70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (ZAP-70 negative) and Ig-unmutated (ZAP-70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The ZAP-70 positive Ig- unmutated CLL cases have a poorer prognosis. Immunohistochemistry (IHC) is a complex technique in which immunological and histological detection methods are combined. In general, the manipulation and processing of tissues before immunostaining, especially different types of tissue fixation and embedding, as well as the nature of the tissues themselves may cause inconsistent results (Nadji and Morales, 1983). Endogenous pseudoperoxidase and peroxidase activity or endogenous biotin and alkaline phosphatase activity can cause non-specific staining results depending on the detection system used. Tissues that contain Hepatitis B surface antigen (HBsAg) can produce false positives when using HRP detection systems (Omata et al, 1980). Insufficient contrast staining and/or improper mounting of the sample may influence the interpretation of results.|
|Material not included||All reagents, materials, and laboratory equipment for IHC procedures are not provided with this antibody. This includes adhesive slides and cover slips, positive and negative control tissues, Xylene or adequate substitute, ethanol, distilled H2O, heat pretreatment equipment (pressure cooker, steamer, microwave), pipettes, Coplin jars, glass jars, moist chamber, histological baths, negative control reagents, counter-staining solution, mounting materials, and microscope.|
|Immunogen||GST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254.|
|Plasmids, Primers & others|
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|Alternative Name||ZAP-70 (ZAP70 Antibody Abstract)|
Application DetailsProduct Details anti-ZAP70 Antibody Target Details ZAP70 Handling Images back to top
Staining pattern: Cytoplasmic. The interpretation of the stain results is the full responsibility of the user. Any experimental result must be confirmed by a medically established diagnostic product or procedure.
Principles of the procedure: The demonstrations of antigens by IHC is a sequential procedure with several steps involving first the application of a specific antibody for the antigen of interest (primary antibody), then a secondary antibody which joins to the first, an enzyme complex, and the addition of a chromogenic substrate. The sample is washed between each step. Enzymatic activation of the chromogenic substrate creates a visible product where the antigen is located. The results are interpreted using a light microscope. The primary antibody can be used both in manual IHC and with automated immunostainers.
Paraffin-embedded tissue samples should be used. The antibody is also useful for immunostaining frozen tissue samples. Western blot techniques are not recommended.
Antigen retrieval: HIER Citrate Buffer pH 6.5
Working dilution: (only for concentrates) 1:25– 1:100
Incubation: 60 min, RT
Control Tissue: Tonsil
|Restrictions||For Research Use only|
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|Buffer||The preparation contains saline buffer, stabilising and carriers proteins. (pH 7.4)|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store at 2-8 °C until the expiration date printed on product label. Do not use after the expiration date. If fresh solutions are required, these must be prepared immediately prior to use, and will be stable for at least one day at room temperature (20-25°C). Unused portion of antibody preparation should be discarded after one day.If the product is stored under different conditions from those stipulated in these technical indications, the new conditions must be verified by the user. The validity period of the ready to use products when opened, is the same as the expiration date indicated on the label of intact product.|
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