anti-Human ZAP70 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • SRK
  • STCD
  • STD
  • TZK
  • ZAP-70
  • 70kDa
  • AI327364
  • Srk
  • mrtle
  • mur
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • zgc:110383
  • zeta chain of T-cell receptor associated protein kinase 70
  • putative tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta chain of T cell receptor associated protein kinase 70
  • zeta-chain (TCR) associated protein kinase 70kDa
  • zeta chain of T cell receptor associated protein kinase 70kDa L homeolog
  • zeta chain of T cell receptor associated protein kinase 70kDa
  • tyrosine-protein kinase ZAP-70
  • ZAP70
  • Smp_149470
  • Zap70
  • zap70.L
  • zap70
  • LOC707555
  • LOC100342021
  • LOC100390922
  • LOC100542877
Reactivity
Human
637
224
147
14
11
10
8
7
6
6
4
3
1
Host
Mouse
340
279
17
3
2
Clonality
Monoclonal
Conjugate
This ZAP70 antibody is un-conjugated
32
25
24
21
15
14
7
7
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
335
212
205
205
141
137
64
52
48
13
8
7
4
4
2
2
2
2
2
Options
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Catalog No. ABIN1027649
$ 1,489.11
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
16.25679 ABIN1027650 IHC (f) IHC (p) Mouse IgG2a Log in to see 2F3-2 0
4 ABIN6189303 FACS IF IHC (f) PerCP Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
4 ABIN6189304 FACS IF IHC (f) PE Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
4 ABIN6189296 FACS IF IHC (f) APC Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
4 ABIN6189307 FACS IF IHC (f) Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
4 ABIN6189372 FACS IF IHC (f) APC Mouse IgG2a, kappa Log in to see ZAP70-528 0
4 ABIN6189379 FACS IF IHC (f) PerCP Mouse IgG2a, kappa Log in to see ZAP70-528 0
4 ABIN6189380 FACS IF IHC (f) PE Mouse IgG2a, kappa Log in to see ZAP70-528 0
4 ABIN6189383 FACS IF IHC (f) Mouse IgG2a, kappa Log in to see ZAP70-528 0
1 ABIN6189308 FACS IF IHC (f) CF®405S Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189328 FACS IF IHC (f) CF®680R Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189280 FACS IF IHC (f) CF®555 Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189282 FACS IF IHC (f) CF®660R Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189286 FACS IF IHC (f) CF®770 Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189288 FACS IF IHC (f) CF®680 Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189314 FACS IF IHC (f) CF®543 Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189324 FACS IF IHC (f) CF®770 Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189326 FACS IF IHC (f) CF®680 Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189330 FACS IF IHC (f) CF®488A Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189332 FACS IF IHC (f) CF®594 Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Reactivity Human
(637), (224), (147), (14), (11), (10), (8), (7), (6), (6), (4), (3), (1)
Host Mouse
(340), (279), (17), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ZAP70 antibody is un-conjugated
(32), (25), (24), (21), (15), (14), (7), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(335), (212), (205), (205), (141), (137), (64), (52), (48), (13), (8), (7), (4), (4), (2), (2), (2), (2), (2)
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling Images
Purpose This antibody is designed for the specific localization of human ZAP-70 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections. It helps in the detection of CLL subtypes and studies of Severe Combined Immunodeficiency Syndrome.
Characteristics ZAP-70 is an abbrevation for Zeta-chain-associated protein kinase 70 (70 is the molecular weight in kDa). The protein is a member of the protein-tyrosine kinase family. ZAP-70 is normally expressed in T-cells and natural killer cells and has a critical role in the initiation of T-cell signaling. ZAP-70 in B- cells is used as a prognostic marker in identifying different forms of Chronic Lymphocytic Leukemia (CLL). ZAP-70 protein is expressed in leukemic cells in approximately 25% of Chronic Lymphocytic Leukemia (CLL) cases as well. ZAP-70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (ZAP-70 negative) and Ig-unmutated (ZAP-70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The ZAP-70 positive Ig- unmutated CLL cases have a poorer prognosis. Immunohistochemistry (IHC) is a complex technique in which immunological and histological detection methods are combined. In general, the manipulation and processing of tissues before immunostaining, especially different types of tissue fixation and embedding, as well as the nature of the tissues themselves may cause inconsistent results (Nadji and Morales, 1983). Endogenous pseudoperoxidase and peroxidase activity or endogenous biotin and alkaline phosphatase activity can cause non-specific staining results depending on the detection system used. Tissues that contain Hepatitis B surface antigen (HBsAg) can produce false positives when using HRP detection systems (Omata et al, 1980). Insufficient contrast staining and/or improper mounting of the sample may influence the interpretation of results.
Purification Ready-to-use, Prediluted,
Material not included All reagents, materials, and laboratory equipment for IHC procedures are not provided with this antibody. This includes adhesive slides and cover slips, positive and negative control tissues, Xylene or adequate substitute, ethanol, distilled H2O, heat pretreatment equipment (pressure cooker, steamer, microwave), pipettes, Coplin jars, glass jars, moist chamber, histological baths, negative control reagents, counter-staining solution, mounting materials, and microscope.
Immunogen GST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254.
Clone 2F3-2
Isotype IgG2a
Plasmids, Primers & others

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ZAP-70 (ZAP70 Antibody Abstract)
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling Images back to top
Comment

Staining pattern: Cytoplasmic. The interpretation of the stain results is the full responsibility of the user. Any experimental result must be confirmed by a medically established diagnostic product or procedure.
Positive control: Tissue sample from tonsil.
External Negative Control: Tissue sample homologous to the test sample incubated with an antibody isotype not specific for ZAP-70.

Protocol Principles of the procedure: The demonstrations of antigens by IHC is a sequential procedure with several steps involving first the application of a specific antibody for the antigen of interest (primary antibody), then a secondary antibody which joins to the first, an enzyme complex, and the addition of a chromogenic substrate. The sample is washed between each step. Enzymatic activation of the chromogenic substrate creates a visible product where the antigen is located. The results are interpreted using a light microscope. The primary antibody can be used both in manual IHC and with automated immunostainers.

Paraffin-embedded tissue samples should be used. The antibody is also useful for immunostaining frozen tissue samples. Western blot techniques are not recommended.
Assay Procedure Antigen retrieval: HIER Citrate Buffer pH 6.5
Working dilution: (only for concentrates) 1:25– 1:100
Incubation: 60 min, RT
Control Tissue: Tonsil
Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Images back to top
Format Liquid
Buffer The preparation contains saline buffer, stabilising and carriers proteins. (pH 7.4)
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 2-8 °C until the expiration date printed on product label. Do not use after the expiration date. If fresh solutions are required, these must be prepared immediately prior to use, and will be stable for at least one day at room temperature (20-25°C). Unused portion of antibody preparation should be discarded after one day.If the product is stored under different conditions from those stipulated in these technical indications, the new conditions must be verified by the user. The validity period of the ready to use products when opened, is the same as the expiration date indicated on the label of intact product.

Images

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody (ABIN1027649) 10 × 20 × IHC of ZAP-70 (clone 2F3.2) on FFPE tonsil tissue. Citrate pH 6.5, DAB, Hem...