anti-Human ZAP70 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • SRK
  • STCD
  • STD
  • TZK
  • ZAP-70
  • 70kDa
  • AI327364
  • Srk
  • mrtle
  • mur
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • zgc:110383
  • zeta chain of T-cell receptor associated protein kinase 70
  • putative tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta chain of T cell receptor associated protein kinase 70
  • zeta-chain (TCR) associated protein kinase 70kDa
  • zeta chain of T cell receptor associated protein kinase 70kDa L homeolog
  • zeta chain of T cell receptor associated protein kinase 70kDa
  • tyrosine-protein kinase ZAP-70
  • ZAP70
  • Smp_149470
  • Zap70
  • zap70.L
  • zap70
  • LOC707555
  • LOC100342021
  • LOC100390922
  • LOC100542877
Epitope
AA 1-254
100
52
51
34
31
25
24
14
12
12
11
11
10
7
7
7
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
641
225
147
14
11
10
8
7
6
6
4
3
1
Host
Mouse
339
284
17
3
2
Clonality
Monoclonal
Conjugate
This ZAP70 antibody is conjugated to CF®405S
33
27
25
21
15
14
7
7
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
338
216
206
204
140
137
64
52
50
17
8
7
4
4
3
2
2
2
2
Supplier
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Catalog No. ABIN6189308
$ 209.23
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6189288 FACS IF IHC (f) CF®680 Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189286 FACS IF IHC (f) CF®770 Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189290 FACS IF IHC (f) CF®680R Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189284 FACS IF IHC (f) CF®568 Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189282 FACS IF IHC (f) CF®660R Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189280 FACS IF IHC (f) CF®555 Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189274 FACS IF IHC (f) CF®640R Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189278 FACS IF IHC (f) CF®647 Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189276 FACS IF IHC (f) CF®543 Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189330 FACS IF IHC (f) CF®488A Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189332 FACS IF IHC (f) CF®594 Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189328 FACS IF IHC (f) CF®680R Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189326 FACS IF IHC (f) CF®680 Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189324 FACS IF IHC (f) CF®770 Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189318 FACS IF IHC (f) CF®555 Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189316 FACS IF IHC (f) CF®647 Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189312 FACS IF IHC (f) CF®640R Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189310 FACS IF IHC (f) CF405M Mouse IgG AA 1-254 Log in to see ZAP70-528 2F3-2 0
1 ABIN6189294 FACS IF IHC (f) CF®594 Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0
1 ABIN6189292 FACS IF IHC (f) CF®488A Mouse IgG2a, kappa AA 1-254 Log in to see 2F3-2 0

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope AA 1-254
(100), (52), (51), (34), (31), (25), (24), (14), (12), (12), (11), (11), (10), (7), (7), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(641), (225), (147), (14), (11), (10), (8), (7), (6), (6), (4), (3), (1)
Host Mouse
(339), (284), (17), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ZAP70 antibody is conjugated to CF®405S
(33), (27), (25), (21), (15), (14), (7), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
(338), (216), (206), (204), (140), (137), (64), (52), (50), (17), (8), (7), (4), (4), (3), (2), (2), (2), (2)
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling Images
Purpose Mouse Monoclonal anti-ZAP70 (ZAP70/528 + 2F3.2), CF405S Conjugate
Characteristics ZAP70 is a 70 kDa protein tyrosine kinase found in T-cells and natural killer cells.Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25 % of chronic lymphocytic leukemia (CLL) cases as well.Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Recombinant full-length human ZAP70 protein (ZAP70/528), Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70 (2F3.2)
Clone ZAP70-528 2F3-2
Isotype IgG
Plasmids, Primers & others

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ZAP70 (ZAP70 Antibody Abstract)
Background Selective T cell defect, SRK, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70,TZK, Zeta chain associated protein kinase 70  kDa
Molecular Weight 70 kDa
Gene ID 7535, 234569
UniProt P43403
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling Images back to top
Application Notes Immunohistology formalin-fixed 0.5-1 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Immunofluorescence 0.5-1 μg/mL
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Optimal dilution for a specific application should be determined by user
Comment

Jurkat cells. Tonsil or lymph node.

Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light