anti-Human ZAP70 antibody for Immunoprecipitation

Recommended ZAP70 Antibody (supplied by: Log in to see )

Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
  • SRK
  • STCD
  • STD
  • TZK
  • ZAP-70
  • 70kDa
  • AI327364
  • Srk
  • mrtle
  • mur
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • zgc:110383
  • zeta chain of T-cell receptor associated protein kinase 70
  • putative tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta chain of T cell receptor associated protein kinase 70
  • zeta-chain (TCR) associated protein kinase 70kDa
  • zeta chain of T cell receptor associated protein kinase 70kDa L homeolog
  • zeta chain of T cell receptor associated protein kinase 70kDa
  • tyrosine-protein kinase ZAP-70
  • ZAP70
  • Smp_149470
  • Zap70
  • zap70.L
  • zap70
  • LOC707555
  • LOC100342021
  • LOC100390922
  • LOC100542877
Epitope
Internal Region
101
52
51
34
31
25
24
14
12
11
11
11
10
7
7
7
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
641
225
147
14
11
10
8
7
6
6
4
3
1
Host
Goat
339
285
16
3
2
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
33
27
25
21
15
14
7
7
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
337
217
206
203
141
137
65
52
49
17
8
7
4
4
3
2
2
2
2
Options
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Catalog No. ABIN987274
$ 358.02
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1875382 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4366557 IHC IHC (p) IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN205364 FACS IP WB Mouse IgG1 AA 282-307 Log in to see 1E7-2 0
1 ABIN438732 FACS IP Rabbit pTyr319 Log in to see Polyclonal 0
1 ABIN438734 IP Rabbit pTyr494 Log in to see Polyclonal 0
1 ABIN1105091 ELISA IHC IHC (p) IP WB Mouse IgG1, kappa Log in to see 0
1 ABIN4366555 CyTOF FACS ICC IF IP WB Mouse IgG1 Log in to see 1E7-2 0
1 ABIN214953 IP ELISA WB Mouse IgG1, kappa AA 326-341 Log in to see 0
1 ABIN218909 IP Rabbit IgG Log in to see Polyclonal 0
1 ABIN210659 ICC IP WB Mouse IgG1 AA 282-307 Log in to see 0
1 ABIN221118 ICC FACS IF IP WB Rabbit IgG pTyr319 Log in to see Polyclonal 0
1 ABIN218908 FACS IP ELISA WB Rabbit IgG pTyr493 Log in to see Polyclonal 0
1 ABIN2663949 FACS IP IHC PE Mouse IgG1 kappa, IgG1 Log in to see 1E7-2 4
1 ABIN212704 FACS IP WB Mouse IgG2a Log in to see 0
1 ABIN2880178 ICC IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5542964 IP WB Mouse IgG2b Log in to see 4B2-C1-H3 0
1 ABIN1959471 FACS IP Rabbit IgG pTyr319 Log in to see Polyclonal 0
1 ABIN1724502 IHC (p) IP ELISA WB Mouse IgG1 kappa Log in to see 0
1 ABIN1959475 IP Rabbit IgG pTyr493 Log in to see Polyclonal 0
1 ABIN1959474 IP Rabbit IgG pTyr493 Log in to see Polyclonal 0

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antibodies
Epitope Internal Region
(101), (52), (51), (34), (31), (25), (24), (14), (12), (11), (11), (11), (10), (7), (7), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(641), (225), (147), (14), (11), (10), (8), (7), (6), (6), (4), (3), (1)
Host Goat
(339), (285), (16), (3), (2)
Clonality
Polyclonal
Conjugate This ZAP70 antibody is un-conjugated
(33), (27), (25), (21), (15), (14), (7), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(337), (217), (206), (203), (141), (137), (65), (52), (49), (17), (8), (7), (4), (4), (3), (2), (2), (2), (2)
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Product Details anti-ZAP70 Antibody

Target Details ZAP70 Application Details Handling Images
Purpose ZAP70
Blocking Peptide Blocking peptide for this product available: ABIN2816708
Sequence SDGYTPEPAR ITSPD
Specificity This antibody is expected to recognize isoform 1 (NP_001070.2) only.
Predicted Reactivity Human, Mouse, Rat, Dog, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-SDGYTPEPARITSPD, from the internal region of the protein sequence according to NP_001070.2.
Plasmids, Primers & others

Target Details ZAP70

Product Details anti-ZAP70 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ZAP70 (ZAP70 Antibody Abstract)
Background ZAP70, zeta-chain (TCR) associated protein kinase 70 kDa, FLJ17670, FLJ17679, SRK, STD, TZK, ZAP-70, 70 kDa zeta-associated protein, syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, zeta-chain associated protein kinase, 70kD
Gene ID 7535
NCBI Accession NP_001070
Pathways TCR Signaling

Application Details

Product Details anti-ZAP70 Antibody Target Details ZAP70 Handling Images back to top
Application Notes

DS WB Results: Approx 70 kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9 kDa according to NP_001070.2). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:32000.

Comment

Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).

Restrictions For Research Use only

Handling

Product Details anti-ZAP70 Antibody Target Details ZAP70 Application Details Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.