anti-Rabbit Histone Cluster 2, H2aa3 antibody for Western Blotting

Recommended Histone Cluster 2, H2aa3 Antibody (supplied by: Log in to see )

Antigen
Histone Cluster 2, H2aa3 (HIST2H2AA3) Antibodies
  • H2A
  • H2A.2
  • H2A/O
  • H2A/q
  • H2AFO
  • H2a-615
  • HIST2H2AA
  • Hist2h2aa
  • Hist2h2aa1
  • histone cluster 2 H2A family member a3
  • histone cluster 2 H2A family member A3
  • HIST2H2AA3
  • Hist2h2aa3
Epitope
Middle Region
10
10
7
7
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
32
2
2
Host
Rabbit
29
3
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
2
2
2
2
Application
Western Blotting (WB)
32
18
4
3
3
1
1
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Catalog No. ABIN2784541
$ 229.00
Plus shipping costs $45.00

General

Antigen Histone Cluster 2, H2aa3 (HIST2H2AA3) Antibodies
Epitope Middle Region
(10), (10), (7), (7), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(32), (2), (2)
Host Rabbit
(29), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(4), (4), (2), (2), (2), (2)
Application Western Blotting (WB)
(32), (18), (4), (3), (3), (1), (1)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Sequence PRHLQLAIRN DEELNKLLGK VTIAQGGVLP NIQAVLLPKK TESHHKAKGK
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against HIST2H2AA3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human HIST2H2AA3

Target Details

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Antigen
Alternative Name HIST2H2AA3 (HIST2H2AA3 Antibody Abstract)
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. HIST2H2AA3 is a member of the histone H2A family.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated, this record represents the centromeric copy. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: H2A, H2A.2, H2A/O, H2A/q, H2AFO, H2a-615, HIST2H2AA
Protein Interaction Partner: USP21, DNAJC2, USP16, AURKB, UBC, WWOX, BAP1, SUZ12, RNF2, HCVgp1, PRMT6, IL33, APP, NOC2L, MEN1, SUMO2, RNF20, MSL2, BMI1, RNF168, HDAC1, PARP1, SRRM1, G3BP1, UBE2H, RCC1, TBL1X,
Protein Size: 130
Molecular Weight 14 kDa
Gene ID 8337
NCBI Accession NM_003516, NP_003507
UniProt Q7L7L0
Pathways Telomere Maintenance

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 130 AA

Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Background publications

Gregory, Barlow, McLay, Kaul, Swarbreck, Dunham, Scott, Howe, Woodfine, Spencer, Jones, Gillson, Searle, Zhou, Kokocinski, McDonald, Evans, Phillips, Atkinson, Cooper, Jones, Hall, Andrews, Lloyd et al.: "The DNA sequence and biological annotation of human chromosome 1. ..." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006

Images

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Supplier Images
Western Blotting (WB) image for anti-Histone Cluster 2, H2aa3 (HIST2H2AA3) (Middle Region) antibody (ABIN2784541) WB Suggested Anti-HIST2H2AA3 Antibody Titration: 2.5ug/ml Positive Control: Jurkat...