anti-Human RFC2 antibody for Enzyme Immunoassay

Recommended RFC2 Antibody (supplied by: Log in to see )

Antigen
Replication Factor C (Activator 1) 2, 40kDa (RFC2) Antibodies
  • 2610008M13Rik
  • 40kDa
  • AI326953
  • Recc2
  • RFC40
  • replication factor C subunit 2
  • replication factor C (activator 1) 2
  • LOC100231971
  • RFC2
  • Rfc2
Epitope
N-Term
15
14
6
5
4
4
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
84
40
38
7
4
4
4
3
3
3
1
1
Host
Rabbit
56
26
2
Clonality
Polyclonal
Conjugate
This RFC2 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
69
22
18
18
13
13
9
4
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Catalog No. ABIN357963
$ 390.50
Plus shipping costs $45.00

General

Antigen Replication Factor C (Activator 1) 2, 40kDa (RFC2) Antibodies
Epitope N-Term
(15), (14), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(84), (40), (38), (7), (4), (4), (4), (3), (3), (3), (1), (1)
Host Rabbit
(56), (26), (2)
Clonality
Polyclonal
Conjugate This RFC2 antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(69), (22), (18), (18), (13), (13), (9), (4)
Pubmed 2 references available
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Product Details anti-RFC2 Antibody

Target Details RFC2 Application Details Handling References for anti-RFC2 antibody (ABIN357963) Images
Specificity This antibody detects RFC2 (N-term).
Purification Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human RFC2.
Isotype Ig Fraction
Plasmids, Primers & others

Target Details RFC2

Product Details anti-RFC2 Antibody Application Details Handling References for anti-RFC2 antibody (ABIN357963) Images back to top
Antigen
Alternative Name RFC2 / RFC40 (RFC2 Antibody Abstract)
Background The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. RFC2 is the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of RFC2 gene has been associated with Williams syndrome.Synonyms: Activator 1 40 kDa subunit, Activator 1 subunit 2, RF-C 40 kDa subunit, Replication factor C 40 kDa subunit, Replication factor C subunit 2
Molecular Weight 39157 Da
Gene ID 5982, 9606
UniProt P35250
Pathways Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA

Application Details

Product Details anti-RFC2 Antibody Target Details RFC2 Handling References for anti-RFC2 antibody (ABIN357963) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-RFC2 Antibody Target Details RFC2 Application Details References for anti-RFC2 antibody (ABIN357963) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-RFC2 antibody (ABIN357963)

Product Details anti-RFC2 Antibody Target Details RFC2 Application Details Handling Images back to top
Background publications

Tomida, Masuda, Hiroaki, Ishikawa, Song, Tsurimoto, Tateishi, Shiomi, Kamei, Kim, Kamiya, Vaziri, Ohmori, Todo: "DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex." in: The Journal of biological chemistry, Vol. 283, Issue 14, pp. 9071-9, 2008

Gupte, Weng, Liu, Lee: "The second subunit of the replication factor C complex (RFC40) and the regulatory subunit (RIalpha) of protein kinase A form a protein complex promoting cell survival." in: Cell cycle (Georgetown, Tex.), Vol. 4, Issue 2, pp. 323-9, 2005

Images

Product Details anti-RFC2 Antibody Target Details RFC2 Application Details Handling References for anti-RFC2 antibody (ABIN357963) back to top
Supplier Images
 image for anti-Replication Factor C (Activator 1) 2, 40kDa (RFC2) (N-Term) antibody (ABIN357963) anti-Replication Factor C (Activator 1) 2, 40kDa (RFC2) (N-Term) antibody