anti-Dog (Canine) Replication Factor C (Activator 1) 3, 38kDa antibody for ELISA

Recommended Replication Factor C (Activator 1) 3, 38kDa Antibody (supplied by: Log in to see )

Antigen
Replication Factor C (Activator 1) 3, 38kDa (RFC3) Antibodies
  • CG5313
  • DRFC
  • DmRFC3
  • Dmel\\CG5313
  • Rfc3
  • 21.m02902
  • 2810416I22Rik
  • 38kDa
  • AU022547
  • Recc3
  • cb275
  • RFC38
  • Replication factor C subunit 3
  • replication factor C3
  • replication factor C subunit 3
  • replication factor C 38 kDa subunit
  • DNA replication factor C complex subunit Rfc3
  • replication factor C3, putative
  • replication factor C (activator 1) 3
  • replication factor C subunit 3 L homeolog
  • RfC3
  • PF14_0601
  • Chro.30359
  • PB000006.01.0
  • PC000345.04.0
  • TP04_0380
  • Tb09.211.3310
  • PY04255
  • PVX_117300
  • BBOV_IV003080
  • SJAG_02182
  • PKH_124240
  • rfc3
  • Rfc3
  • RFC3
  • rfc3.L
Reactivity
Dog (Canine), Human
60
27
25
9
7
5
4
3
3
3
3
2
1
1
1
Host
Rabbit
49
10
1
Clonality
Polyclonal
Application
ELISA, Western Blotting (WB)
58
21
14
10
9
7
4
3
1
Supplier
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Catalog No. ABIN2462934
$ 446.88
Plus shipping costs $45.00

General

Antigen Replication Factor C (Activator 1) 3, 38kDa (RFC3) Antibodies
Reactivity Dog (Canine), Human
(60), (27), (25), (9), (7), (5), (4), (3), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(49), (10), (1)
Clonality
Polyclonal
Application ELISA, Western Blotting (WB)
(58), (21), (14), (10), (9), (7), (4), (3), (1)
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Product details

Target Details Application Details Handling Images
Purification Antibody is purified by peptide affinity chromatography method.
Immunogen Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFC3.

Target Details

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Antigen
Alternative Name RFC3 (RFC3 Antibody Abstract)
Background The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. RFC3 is the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 38 kD subunit. This subunit is essential for the interaction between the 140 kD subunit and the core complex that consists of the 36, 37, and 40 kD subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Molecular Weight 40 kDa
Gene ID 5983
NCBI Accession NP_002906
UniProt P40938
Pathways Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA

Application Details

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Application Notes RFC3 antibody can be used for detection of RFC3 by ELISA at 1:62500. RFC3 antibody can be used for detection of RFC3 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions For Research Use only

Handling

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Format Lyophilized
Reconstitution Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration 1 mg/mL
Buffer Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice As with any antibody avoid repeat freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment For short periods of storage (days) store at 4 °C. For longer periods of storage, store RFC3 antibody at -20 °C.

Images

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Supplier Images
Western Blotting (WB) image for anti-Replication Factor C (Activator 1) 3, 38kDa (RFC3) antibody (ABIN2462934) anti-Replication Factor C (Activator 1) 3, 38kDa (RFC3) antibody