anti-Human Replication Factor C (Activator 1) 3, 38kDa antibody for Immunoprecipitation

Recommended Replication Factor C (Activator 1) 3, 38kDa Antibody (supplied by: Log in to see )

Antigen
Replication Factor C (Activator 1) 3, 38kDa (RFC3) Antibodies
  • CG5313
  • DRFC
  • DmRFC3
  • Dmel\\CG5313
  • Rfc3
  • 21.m02902
  • 2810416I22Rik
  • 38kDa
  • AU022547
  • Recc3
  • cb275
  • RFC38
  • Replication factor C subunit 3
  • replication factor C3
  • replication factor C subunit 3
  • replication factor C 38 kDa subunit
  • DNA replication factor C complex subunit Rfc3
  • replication factor C3, putative
  • replication factor C (activator 1) 3
  • replication factor C subunit 3 L homeolog
  • RfC3
  • PF14_0601
  • Chro.30359
  • PB000006.01.0
  • PC000345.04.0
  • TP04_0380
  • Tb09.211.3310
  • PY04255
  • PVX_117300
  • BBOV_IV003080
  • SJAG_02182
  • PKH_124240
  • rfc3
  • Rfc3
  • RFC3
  • rfc3.L
Epitope
AA 257-356
7
6
5
4
4
4
3
3
2
2
2
2
1
1
1
1
Reactivity
Human
60
27
25
9
8
5
4
3
3
3
3
2
1
1
1
Host
Mouse
50
9
1
Clonality
Monoclonal
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
58
21
14
10
9
7
3
3
1
Options
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Catalog No. ABIN394944
$ 461.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19.380936 ABIN519745 IP ELISA WB Mouse IgG2a kappa AA 257-356, partial Log in to see 1C6 0
1 ABIN1828770 IP ELISA WB Mouse IgG2a, kappa AA 257-356 Log in to see 1C6 0
1 ABIN2773249 ELISA IP WB Mouse IgG2a kappa Log in to see 0

General

Antigen Replication Factor C (Activator 1) 3, 38kDa (RFC3) Antibodies
Epitope AA 257-356
(7), (6), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(60), (27), (25), (9), (8), (5), (4), (3), (3), (3), (3), (2), (1), (1), (1)
Host Mouse
(50), (9), (1)
Clonality (Clone)
Monoclonal   ( )
Application ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(58), (21), (14), (10), (9), (7), (3), (3), (1)
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Product details

Target Details Application Details Handling Images
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: RFC3 (AAH00149, 257 a.a. ~ 356 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 1C6
Isotype IgG2a kappa

Target Details

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Antigen
Alternative Name RFC3 (RFC3 Antibody Abstract)
Background

Other Names: Replication factor C subunit 3, Activator 1 38 kDa subunit, A1 38 kDa subunit, Activator 1 subunit 3, Replication factor C 38 kDa subunit, RF-C 38 kDa subunit, RFC38, RFC3

Gene Description: replication factor C (activator 1) 3, 38  kDa

Gene Summary: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq

Molecular Weight 40556 DA
Gene ID 5983
NCBI Accession NP_002906, NP_853536
UniProt P40938
Pathways Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA

Application Details

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Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Restrictions For Research Use only

Handling

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Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

Images

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Supplier Images
Western Blotting (WB) image for anti-Replication Factor C (Activator 1) 3, 38kDa (RFC3) (AA 257-356) antibody (ABIN394944) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Immunoprecipitation (IP) image for anti-Replication Factor C (Activator 1) 3, 38kDa (RFC3) (AA 257-356) antibody (ABIN394944) Immunoprecipitation of RFC3 transfected lysate using anti-RFC3 monoclonal antibody an...
ELISA image for anti-Replication Factor C (Activator 1) 3, 38kDa (RFC3) (AA 257-356) antibody (ABIN394944) Detection limit for recombinant GST tagged RFC3 is approximately 10ng/ml as a capture...