anti-Rat (Rattus) Replication Factor C (Activator 1) 3, 38kDa antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Replication Factor C (Activator 1) 3, 38kDa Antibody (supplied by: Log in to see )

Antigen
Replication Factor C (Activator 1) 3, 38kDa (RFC3) Antibodies
  • CG5313
  • DRFC
  • DmRFC3
  • Dmel\\CG5313
  • Rfc3
  • 21.m02902
  • 2810416I22Rik
  • 38kDa
  • AU022547
  • Recc3
  • cb275
  • RFC38
  • Replication factor C subunit 3
  • replication factor C3
  • replication factor C subunit 3
  • replication factor C 38 kDa subunit
  • DNA replication factor C complex subunit Rfc3
  • replication factor C3, putative
  • replication factor C (activator 1) 3
  • replication factor C subunit 3 L homeolog
  • RfC3
  • PF14_0601
  • Chro.30359
  • PB000006.01.0
  • PC000345.04.0
  • TP04_0380
  • Tb09.211.3310
  • PY04255
  • PVX_117300
  • BBOV_IV003080
  • SJAG_02182
  • PKH_124240
  • rfc3
  • Rfc3
  • RFC3
  • rfc3.L
Reactivity
Human, Mouse (Murine), Rat (Rattus)
60
26
24
9
8
5
4
3
3
3
3
2
1
1
1
Host
Rabbit
49
10
1
Clonality
Polyclonal
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
58
22
14
9
9
7
4
3
1
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Catalog No. ABIN498529
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5961885 ELISA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2892894 IHC IHC (p) WB Rabbit Leu227 Log in to see Polyclonal 0
1 ABIN2887786 ELISA IHC IHC (p) WB Rabbit IgG AA 178-227 Log in to see Polyclonal 0
1 ABIN2627623 IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 0
1 ABIN1930251 IF IHC IHC (p) WB Rabbit IgG AA 80-110 Log in to see Polyclonal 0
1 ABIN1930252 IF IHC IHC (p) Rabbit IgG AA 80-110 Log in to see Polyclonal 0

General

Antigen Replication Factor C (Activator 1) 3, 38kDa (RFC3) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(60), (26), (24), (9), (8), (5), (4), (3), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(49), (10), (1)
Clonality
Polyclonal
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(58), (22), (14), (9), (9), (7), (4), (3), (1)
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Product details

Target Details Application Details Handling Images
Specificity This antibody detects endogenous levels of RFC3 protein. (region surrounding Leu227)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography using epitope-specific immunogen
Purity > 95 % (by SDS-PAGE)

Target Details

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Antigen
Alternative Name RFC3 / RFC38 (RFC3 Antibody Abstract)
Background The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 38 kD subunit. This subunit is essential for the interaction between the 140 kD subunit and the core complex that consists of the 36, 37, and 40 kD subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.Synonyms: Activator 1 38 kDa subunit, Activator 1 subunit 3, RF-C 38 kDa subunit, Replication factor C 38 kDa subunit, Replication factor C subunit 3
Molecular Weight approx. 41 kDa
Gene ID 5983
NCBI Accession NP_002906
UniProt P40938
Pathways Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA

Application Details

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Application Notes Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

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Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Images

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Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Replication Factor C (Activator 1) 3, 38kDa (RFC3) antibody (ABIN498529) Immunohistochemistry analyzes of RFC3 antibody in paraffin-embedded human brai...
Western Blotting (WB) image for anti-Replication Factor C (Activator 1) 3, 38kDa (RFC3) antibody (ABIN498529) Western blot analysis of RFC3 antibody in extracts from HepG2 cells.