anti-Cow (Bovine) PKC alpha antibody for Immunoprecipitation

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • pkc-alpha
  • pkcalpha
  • im:7139045
  • si:dkey-8l19.1
  • LOC100219195
  • AAG6
  • PKC-alpha
  • PKCA
  • PRKACA
  • AI875142
  • Pkca
  • PKRCA
  • protein kinase C alpha
  • protein kinase C, alpha L homeolog
  • protein kinase C, alpha
  • PRKCA
  • prkca.L
  • prkca
  • Prkca
Reactivity
Cow (Bovine), Dog (Canine), Fish, Human, Mouse (Murine), Rat (Rattus)
306
219
175
30
25
25
21
21
19
5
3
3
2
1
1
1
1
Host
Mouse
275
43
2
Clonality
Monoclonal
Conjugate
This PKC alpha antibody is un-conjugated
14
13
10
7
6
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
260
151
135
69
55
50
32
31
26
23
13
5
4
4
2
1
1
1
Supplier
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Catalog No. ABIN331530
$ 463.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1926563 IP WB Rabbit IgG AA 622-672 Log in to see Polyclonal 0

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Reactivity Cow (Bovine), Dog (Canine), Fish, Human, Mouse (Murine), Rat (Rattus)
(306), (219), (175), (30), (25), (25), (21), (21), (19), (5), (3), (3), (2), (1), (1), (1), (1)
Host Mouse
(275), (43), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PKC alpha antibody is un-conjugated
(14), (13), (10), (7), (6), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(260), (151), (135), (69), (55), (50), (32), (31), (26), (23), (13), (5), (4), (4), (2), (1), (1), (1)
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling Images
Specificity Detects an ~80kD protein, corresponding to the apparent molecular mass of PKC alpha on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, fish (rainbow trout), guinea pig, monkey, pig, rabbit and sheep origins. The epitope of this antibody is within the variable region V3 of PKC alpha (the site of limited proteolysis on the native enzyme). This antibody is specific for PKC alpha and does not detect the other PKC isoforms.
Purification Protein G purified
Immunogen Bovine brain PKCa.
Clone MC5
Isotype IgG2a
Plasmids, Primers & others

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRKCA / PKC-alpha (PKCa Antibody Abstract)
Background Name/Gene ID: PRKCA
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCA, Aging-associated gene 6, AAG6, Alpha PKC, PKC-A, Pkcalpha, Protein kinase C alpha type, PKC Alpha, PKC-alpha, PKCA, Protein kinase c alpha, Protein kinase C, alpha
Gene ID 5578
Pathways WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling Images back to top
Application Notes Approved: Flo (10 μg/mL), IHC (1:50), IP (12.5 μg/mL), WB (1 μg/mL)

Not recommended for: IHC-P
Comment

Target Species of Antibody: Bovine

Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS 0.1 mM PMSF, 50 % glycerol
Preservative PMSF
Handling Advice avoid freeze thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.