anti-Human ROCK1 antibody for ELISA

Recommended ROCK1 Antibody (supplied by: Log in to see )

Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
  • LOC100229794
  • CG9774
  • DROK
  • DRhk
  • DRok
  • Dmel\\CG9774
  • Drok
  • ROCK
  • ROCK-1
  • ROCK1
  • ROK
  • ROKalpha/beta
  • Rhk
  • RhoK
  • Rock
  • Rok
  • dROK
  • dRok
  • drok
  • 1110055K06Rik
  • Rock-I
  • P160Rock
  • ROCK-I
  • P160ROCK
  • p160ROCK
  • Rho associated coiled-coil containing protein kinase 1
  • Rho kinase
  • Rho-associated coiled-coil containing protein kinase 1
  • Rho-associated, coiled-coil containing protein kinase 1
  • ROCK1
  • Rok
  • Rock1
  • rock1
Epitope
AA 401-510, partial
39
27
27
24
21
15
5
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
194
143
133
25
21
20
6
5
4
2
1
Host
Mouse
113
83
Clonality
Monoclonal
Conjugate
This ROCK1 antibody is un-conjugated
8
7
7
7
6
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
153
46
36
33
28
25
25
14
2
2
1
1
1
1
Supplier
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Catalog No. ABIN562671
$ 440.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10.764514 ABIN969563 ELISA WB Mouse IgG1 Log in to see 1H4 2
10.764514 ABIN4350896 ELISA WB Mouse IgG1 Log in to see 1H4 0
10.764514 ABIN1846125 ELISA WB Mouse IgG1 Log in to see 0
1 ABIN1500698 ELISA WB Mouse IgG1 Log in to see 1H4 0
1 ABIN3186829 ELISA ICC IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN948412 ELISA WB Mouse IgG1 kappa AA 401-510, partial Log in to see 2E2 0
1 ABIN1031544 ELISA WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN1453860 IHC ELISA WB Rabbit IgG AA 262-311 Log in to see Polyclonal 0
1 ABIN4350897 ELISA ICC IF WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN4881029 ELISA WB Mouse IgG1 Log in to see 1H4 0
1 ABIN1828788 IF ELISA WB Mouse IgG1, kappa AA 401-510 Log in to see 2E2 0
1 ABIN5682305 ELISA WB Mouse IgG1 Log in to see 1H4 0
1 ABIN1585151 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN3186828 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN2885883 IHC ELISA WB Rabbit IgG AA 262-311 Log in to see Polyclonal 0
1 ABIN2353153 ELISA WB Mouse IgG1 Log in to see 13B679 0
1 ABIN2353148 IC IF ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN2751305 ELISA IHC IP WB FITC Rabbit IgG AA 1300-1360 Log in to see Polyclonal 0
1 ABIN2751304 ELISA IHC IP WB Rabbit IgG AA 1300-1360 Log in to see Polyclonal 0
1 ABIN2353154 IF ELISA WB Mouse IgG1 kappa AA 401-511 Log in to see 2E2 0

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies
Epitope AA 401-510, partial
(39), (27), (27), (24), (21), (15), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(194), (143), (133), (25), (21), (20), (6), (5), (4), (2), (1)
Host Mouse
(113), (83)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ROCK1 antibody is un-conjugated
(8), (7), (7), (7), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(153), (46), (36), (33), (28), (25), (25), (14), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-ROCK1 Antibody

Target Details ROCK1 Application Details Handling References for anti-ROCK1 antibody (ABIN562671) Images
Cross-Reactivity Human
Immunogen ROCK1 (NP_005397, 401 a.a. ~ 510 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen sequence: SNRRYLSSAN PNDNRTSSNA DKSLQESLQK TIYKLEEQLH NEMQLKDEME QKCRTSNIKL DKIMKELDEE GNQRRNLEST VSQIEKEKML LQHRINEYQR KAEQENEKRR
Clone 2E2
Isotype IgG1 kappa

Target Details ROCK1

Product Details anti-ROCK1 Antibody Application Details Handling References for anti-ROCK1 antibody (ABIN562671) Images back to top
Antigen
Alternative Name ROCK1 (ROCK1 Antibody Abstract)
Background Full Gene Name: Rho-associated, coiled-coil containing protein kinase 1
Synonyms: MGC131603,MGC43611,P160ROCK,PRO0435
Gene ID 6093
NCBI Accession NP_005397, NM_005406
Pathways Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-ROCK1 Antibody Target Details ROCK1 Handling References for anti-ROCK1 antibody (ABIN562671) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Product Quality tested by: Antibody Reactive Against Recombinant Protein.

Restrictions For Research Use only

Handling

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details References for anti-ROCK1 antibody (ABIN562671) Images back to top
Buffer In 1x PBS, pH 7.4
Handling Advice Aliquot to avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store at -20 °C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-ROCK1 antibody (ABIN562671)

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Handling Images back to top
Product cited in:

Nishimura, Bereczky, Yoshioka, Taniguchi, Itoh: "A novel role of Rho-kinase in the regulation of ligand-induced phosphorylated EGFR endocytosis via the early/late endocytic pathway in human fibrosarcoma cells." in: Journal of molecular histology, Vol. 42, Issue 5, pp. 427-42, 2011

Gong, Fang, Peng, Liu, Du: "Integration of virtual screening with high-throughput screening for the identification of novel Rho-kinase I inhibitors." in: Journal of biotechnology, Vol. 145, Issue 3, pp. 295-303, 2010

Images

Product Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Handling References for anti-ROCK1 antibody (ABIN562671) back to top
Supplier Images
Immunofluorescence (IF) image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 401-510), (partial) antibody (ABIN562671) Immunofluorescence of monoclonal antibody to ROCK1 on HeLa cell. [antibody concentrat...
Western Blotting (WB) image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 401-510), (partial) antibody (ABIN562671) ROCK1 monoclonal antibody (M01), clone 2E2 Western Blot analysis of ROCK1 expression ...
ELISA image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 401-510), (partial) antibody (ABIN562671) Detection limit for recombinant GST tagged ROCK1 is approximately 0.03ng/ml as a capt...
 image for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (AA 401-510), (partial) antibody (ABIN562671) Western Blot detection against Immunogen (37.84 KDa) .