The human cell line blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human cell lines with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane. The cell lines included in the blot are MCF7 (breast, adenocarcinoma), HeLa (cervix, adenocarcinoma), HT-29 (colon, adenocarcinoma), 293 (kidney, transformed with adenovirus 5 DNA), Hep G2 (liver, hepatocellular carcinoma), A-549 (lung, carcinoma), SK-N-SH (brain, neuroblastoma), and PC-3 (prostate,adenocarcinoma).
Samples loaded: Lanes: 1: protein markers, 2: MCF7, 3: HeLa, 4: HT-29, 5: 293, 6: Hep G2, 7: A-549, 8: SK-N-SH, and 9: PC-3.
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %