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|Antigen||CD14 Molecule (CD14) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Cat (Feline), Goat, Human, Mink, Monkey, Pig (Porcine), Rabbit, Sheep (Ovine) Alternatives|
|Conjugate||This CD14 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)
|6 references available|
|Supplier||Log in to see|
Product Details anti-CD14 AntibodyTarget Details CD14 Application Details Handling References for anti-CD14 antibody (ABIN118509) Images
Species reactivity (expected):Canine, Goat, Cat, Rabbit, Mink, Bovine, Porcine,Sheep, Cynomolgus Monkey, Llama.
Species reactivity (tested):Human.
|Purification||Affinity Chromatography on Protein G.|
|Plasmids, Primers & others|
Target Details CD14Product Details anti-CD14 Antibody Application Details Handling References for anti-CD14 antibody (ABIN118509) Images back to top
|Alternative Name||CD14 (CD14 Antibody Abstract)|
|Background||CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidyl-inositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein|
|Pathways||TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-CD14 Antibody Target Details CD14 Handling References for anti-CD14 antibody (ABIN118509) Images back to top
Flow cytometry: 1/50 - 1/200, use 5 μL of suggested working dilution to label 10^6 cells or 100 μL whole blood. ELISA. Western Blot under reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD14 Antibody Target Details CD14 Application Details References for anti-CD14 antibody (ABIN118509) Images back to top
|Buffer||PBS containing 0.09 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-CD14 antibody (ABIN118509)Product Details anti-CD14 Antibody Target Details CD14 Application Details Handling Images back to top
Yang, Lan, Xiao, Chen: "Antibody to CD14 like CXCR4-specific antibody 12G5 could inhibit CXCR4-dependent chemotaxis and HIV Env-mediated cell fusion." in: Immunology letters, Vol. 88, Issue 1, pp. 27-30, 2003
Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000
Werling, Howard, Niederer, Straub, Saalmüller, Langhans: "Analysis of the phenotype and phagocytic activity of monocytes/macrophages from cattle infected with the bovine leukaemia virus." in: Veterinary immunology and immunopathology, Vol. 62, Issue 3, pp. 185-95, 1998
Gupta, McConnell, Dalziel, Hopkins: "Identification of the sheep homologue of the monocyte cell surface molecule--CD14." in: Veterinary immunology and immunopathology, Vol. 51, Issue 1-2, pp. 89-99, 1997
Soell, Lett, Holveck, Schöller, Wachsmann, Klein: "Activation of human monocytes by streptococcal rhamnose glucose polymers is mediated by CD14 antigen, and mannan binding protein inhibits TNF-alpha release." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 2, pp. 851-60, 1995
Jacobsen, Aasted, Broe, Petersen: "Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species." in: Veterinary immunology and immunopathology, Vol. 39, Issue 4, pp. 461-6, 1994
ImagesProduct Details anti-CD14 Antibody Target Details CD14 Application Details Handling References for anti-CD14 antibody (ABIN118509) back to top