anti-Goat CD14 antibody for Enzyme Immunoassay

Recommended CD14 Antibody (supplied by: Log in to see )

Antigen
CD14 Molecule (CD14) Antibodies
  • CD14 molecule
  • CD14 antigen
  • CD14
  • Cd14
Reactivity
Cow (Bovine), Dog (Canine), Cat (Feline), Goat, Human, Mink, Monkey, Pig (Porcine), Rabbit, Sheep (Ovine)
941
122
101
89
89
72
64
63
62
61
58
49
44
41
33
26
21
13
10
9
9
9
4
3
3
1
1
Host
Mouse
859
141
48
24
3
Clonality
Monoclonal
Conjugate
This CD14 antibody is un-conjugated
103
98
73
67
29
16
16
13
13
13
10
9
8
8
8
7
7
7
7
7
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)
828
269
213
204
184
142
68
52
47
42
26
14
13
13
11
8
6
4
3
3
3
3
3
3
2
2
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN118509
$ 478.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
4 ABIN118510 EIA FACS WB Mouse IgG2a Log in to see Tük4 6
1 ABIN319672 EIA FACS Mouse IgG2a Log in to see Tük4 5
1 ABIN118508 EIA Func FACS WB Mouse IgG2a Log in to see Tük4 5

General

Antigen CD14 Molecule (CD14) Antibodies
Reactivity Cow (Bovine), Dog (Canine), Cat (Feline), Goat, Human, Mink, Monkey, Pig (Porcine), Rabbit, Sheep (Ovine)
(941), (122), (101), (89), (89), (72), (64), (63), (62), (61), (58), (49), (44), (41), (33), (26), (21), (13), (10), (9), (9), (9), (4), (3), (3), (1), (1)
Host Mouse
(859), (141), (48), (24), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD14 antibody is un-conjugated
(103), (98), (73), (67), (29), (16), (16), (13), (13), (13), (10), (9), (8), (8), (8), (7), (7), (7), (7), (7), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)
(828), (269), (213), (204), (184), (142), (68), (52), (47), (42), (26), (14), (13), (13), (11), (8), (6), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Pubmed 6 references available
Supplier Log in to see

Product Details anti-CD14 Antibody

Target Details CD14 Application Details Handling References for anti-CD14 antibody (ABIN118509) Images
Cross-Reactivity (Details) Species reactivity (expected):Canine, Goat, Cat, Rabbit, Mink, Bovine, Porcine,Sheep, Cynomolgus Monkey, Llama.
Species reactivity (tested):Human.
Purification Affinity Chromatography on Protein G.
Clone Tük4
Isotype IgG2a
Plasmids, Primers & others

Target Details CD14

Product Details anti-CD14 Antibody Application Details Handling References for anti-CD14 antibody (ABIN118509) Images back to top
Antigen
Alternative Name CD14 (CD14 Antibody Abstract)
Background CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidyl-inositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
Gene ID 9606
UniProt P08571
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades

Application Details

Product Details anti-CD14 Antibody Target Details CD14 Handling References for anti-CD14 antibody (ABIN118509) Images back to top
Application Notes Flow cytometry: 1/50 - 1/200, use 5 μL of suggested working dilution to label 10^6 cells or 100 μL whole blood. ELISA. Western Blot under reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD14 Antibody Target Details CD14 Application Details References for anti-CD14 antibody (ABIN118509) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD14 antibody (ABIN118509)

Product Details anti-CD14 Antibody Target Details CD14 Application Details Handling Images back to top
Background publications

Yang, Lan, Xiao, Chen: "Antibody to CD14 like CXCR4-specific antibody 12G5 could inhibit CXCR4-dependent chemotaxis and HIV Env-mediated cell fusion." in: Immunology letters, Vol. 88, Issue 1, pp. 27-30, 2003

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000

Werling, Howard, Niederer, Straub, Saalmüller, Langhans: "Analysis of the phenotype and phagocytic activity of monocytes/macrophages from cattle infected with the bovine leukaemia virus." in: Veterinary immunology and immunopathology, Vol. 62, Issue 3, pp. 185-95, 1998

Gupta, McConnell, Dalziel, Hopkins: "Identification of the sheep homologue of the monocyte cell surface molecule--CD14." in: Veterinary immunology and immunopathology, Vol. 51, Issue 1-2, pp. 89-99, 1997

Soell, Lett, Holveck, Schöller, Wachsmann, Klein: "Activation of human monocytes by streptococcal rhamnose glucose polymers is mediated by CD14 antigen, and mannan binding protein inhibits TNF-alpha release." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 2, pp. 851-60, 1995

Jacobsen, Aasted, Broe, Petersen: "Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species." in: Veterinary immunology and immunopathology, Vol. 39, Issue 4, pp. 461-6, 1994

Images

Product Details anti-CD14 Antibody Target Details CD14 Application Details Handling References for anti-CD14 antibody (ABIN118509) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD14 Molecule (CD14) antibody (ABIN118509) Staining of human peripheral blood monocytes probed with Mouse anti Human CD14 (ABIN1...