anti-Mouse (Murine) CD36 antibody for Functional Studies

Recommended CD36 Antibody (supplied by: Log in to see )

Antigen
CD36 Molecule (thrombospondin Receptor) (CD36) Antibodies
  • BDPLT10
  • CHDS7
  • FAT
  • GP3B
  • GP4
  • GPIV
  • PASIV
  • SCARB3
  • Fat
  • Scarb3
  • GPIIIB
  • PAS-4
  • zgc:92513
  • CD36 molecule
  • CD36 molecule (thrombospondin receptor)
  • CD36
  • Cd36
  • cd36
Reactivity
Mouse (Murine)
364
151
92
8
7
4
4
3
3
3
1
Host
Mouse
254
139
48
12
2
Clonality
Monoclonal
Conjugate
This CD36 antibody is un-conjugated
53
27
21
20
10
9
8
5
5
5
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
292
143
134
90
65
40
40
39
37
28
14
3
3
3
3
3
3
2
2
2
2
1
1
1
1
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Catalog No. ABIN2191823
$ 473.87
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
5.5 ABIN2480134 FACS Func IF Rat IgG2a Log in to see MF3 4
5.5 ABIN2689209 Func FACS IF IP Mouse IgA, kappa Log in to see CRF D 3
1 ABIN1106326 Func IF IP WB Mouse IgA Log in to see CRF D2712 5
1 ABIN343926 FACS Func Mouse IgA Log in to see JC63-1 0
1 ABIN1819915 Func FACS IF IP WB Rat IgG2a Log in to see MF3 0

General

Antigen CD36 Molecule (thrombospondin Receptor) (CD36) Antibodies
Reactivity Mouse (Murine)
(364), (151), (92), (8), (7), (4), (4), (3), (3), (3), (1)
Host Mouse
(254), (139), (48), (12), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD36 antibody is un-conjugated
(53), (27), (21), (20), (10), (9), (8), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(292), (143), (134), (90), (65), (40), (40), (39), (37), (28), (14), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD36 Antibody

Target Details CD36 Application Details Handling References for anti-CD36 antibody (ABIN2191823) Images
Endotoxin Level Low endotoxin level
Clone CRF D-2712
Plasmids, Primers & others

Target Details CD36

Product Details anti-CD36 Antibody Application Details Handling References for anti-CD36 antibody (ABIN2191823) Images back to top
Antigen
Alternative Name CD36 (CD36 Antibody Abstract)
Background Monoclonal antibody CRF D-2712 (also known as JC63.1 or 63) reacts with mouse CD36 (85- kDa), a cell surface class B scavenger receptor. Recently, CD36 has been identified as a protein that is required for TLR2 recognition of di-acylated bacterial lipopeptides and lipoteichoic acid. CD36 is a heavily N- glycosylated transmembrane protein with two short intracellular domains and a large extracellular domain. CD36 expression is primarily controlled by the transcription heterodimer PPARg-RXR (peroxisome proliferator-activated receptor-g-retinoid-X-receptor). CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signalling and adaptor molecules. CD36 is expressed on monocytes, macrophages, endothelial cells, hepatocytes, erythrocytes, adipocytes, cardiomyocytes and red skeletal muscle. Importantly it is expressed by steriodogenic cells in the adrenal gland, testis and ovary. CD36 has been implicated in many biological processes including angiogenesis, phagocytosis, inflammation, and lipid and glucose metabolism. CD36 is associated with Src-family kinases and with the integrins alpha3beta1 and alpha6beta1. It is a receptor and transporter of oxidized lipids and long chain fatty acids. CD36 has been shown to function as phagocytic receptor for apoptotic cells. Several in vivo models support the role of the thrombospondin / CD36 system in angiogenesis and tumor growth. An important role for CD36 has been found in Malaria as major receptor for P. falciparum- infected red blood cells. Many different ligands have been reported to interact with CD36, suggesting that CD36 could recognize a structure-based domain rather than specific contact residues. Monoclonal antibody CRF D-2712 blocks CD36 binding. It is not known whether the antibody is activating or neutralizing. The antibody cross reacts with rat CD36.
Pathways TLR Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism

Application Details

Product Details anti-CD36 Antibody Target Details CD36 Handling References for anti-CD36 antibody (ABIN2191823) Images back to top
Application Notes For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:25. For functional studies dilutions have to be made according to the amounts of CD36 binding to be blocked.
Restrictions For Research Use only

Handling

Product Details anti-CD36 Antibody Target Details CD36 Application Details References for anti-CD36 antibody (ABIN2191823) Images back to top
Buffer 2 mL(> 100 μg/mL) culture medium with low endotoxin level.
Storage 4 °C
Storage Comment Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Expiry Date 12 months

References for anti-CD36 antibody (ABIN2191823)

Product Details anti-CD36 Antibody Target Details CD36 Application Details Handling Images back to top
Product cited in:

Bastie, Nahlé, McLoughlin, Esser, Zhang, Unterman, Abumrad: "FoxO1 stimulates fatty acid uptake and oxidation in muscle cells through CD36-dependent and -independent mechanisms." in: The Journal of biological chemistry, Vol. 280, Issue 14, pp. 14222-9, 2005

Kunjathoor, Febbraio, Podrez, Moore, Andersson, Koehn, Rhee, Silverstein, Hoff, Freeman: "Scavenger receptors class A-I/II and CD36 are the principal receptors responsible for the uptake of modified low density lipoprotein leading to lipid loading in macrophages." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49982-8, 2002

Smith, Serghides, Patel, Febbraio, Silverstein, Kain: "CD36-mediated nonopsonic phagocytosis of erythrocytes infected with stage I and IIA gametocytes of Plasmodium falciparum." in: Infection and immunity, Vol. 71, Issue 1, pp. 393-400, 2002

Finnemann, Silverstein: "Differential roles of CD36 and alphavbeta5 integrin in photoreceptor phagocytosis by the retinal pigment epithelium." in: The Journal of experimental medicine, Vol. 194, Issue 9, pp. 1289-98, 2001