anti-Mouse (Murine) Interferon Regulatory Factor 3 antibody for Western Blotting

Recommended Interferon Regulatory Factor 3 Antibody (supplied by: Log in to see )

Antigen
Interferon Regulatory Factor 3 (IRF3) Antibodies
  • irf3
  • IRF3
  • C920001K05Rik
  • IRF-3
  • interferon regulatory factor 3
  • interferon regulatory factor 7
  • irf3
  • IRF3
  • irf-3
  • Irf3
  • IRF7
Reactivity
Mouse (Murine), Rat (Rattus), Human
290
122
71
7
6
6
3
2
2
1
1
1
1
Host
Rabbit
257
63
3
Clonality
Polyclonal
Conjugate
Un-conjugated
7
6
6
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
267
129
102
77
51
41
28
27
25
14
14
8
4
3
3
2
2
1
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Catalog No. ABIN3023115
$ 393.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.319984 ABIN742688 IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 375-425, pSer396 Log in to see Polyclonal 4
19.819984 ABIN5998004 IF/ICC IHC WB Rabbit IgG Log in to see Polyclonal 0
16.392225 ABIN3185228 ELISA IHC WB Rabbit IgG Ser608 Log in to see Polyclonal 0
14.5 ABIN682588 IF (p) IHC (p) WB Rabbit IgG AA 60-95 Log in to see Polyclonal 1
13.819985 ABIN5963615 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
12.572241 ABIN3182223 ELISA WB Rabbit IgG pSer396 Log in to see Polyclonal 0
10.819985 ABIN6262652 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
10.819985 ABIN6270186 ELISA IHC WB Rabbit IgG pSer15 Log in to see Polyclonal 0
10.819985 ABIN2957643 IC IF IHC WB Rabbit C-Term, pSer385 Log in to see Polyclonal 0
10.819985 ABIN1868750 ICC IHC WB Rabbit IgG AA 1-406 Log in to see Polyclonal 0
10.819985 ABIN5557495 IF (cc) IHC (p) WB Rabbit IgG Log in to see 4C3 0
10.819985 ABIN2935238 IF/ICC IHC IP WB Rabbit AA 1-406 Log in to see Polyclonal 0
10.819985 ABIN2428297 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
9.319985 ABIN1881465 WB Rabbit AA 46-72, N-Term Log in to see Polyclonal 5
7.819985 ABIN2779404 WB Rabbit C-Term Log in to see Polyclonal 1
7.819985 ABIN1532452 IHC ELISA WB Rabbit IgG AA 362-411 Log in to see Polyclonal 0
7.819985 ABIN6255296 IHC WB Rabbit IgG pSer396 Log in to see Polyclonal 0
7.819985 ABIN1030450 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 0
7 ABIN213923 IF IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
7 ABIN317879 IHC (p) WB Rabbit Log in to see Polyclonal 0

General

Antigen Interferon Regulatory Factor 3 (IRF3) Antibodies
Reactivity Mouse (Murine), Rat (Rattus), Human
(290), (122), (71), (7), (6), (6), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(257), (63), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(7), (6), (6), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(267), (129), (102), (77), (51), (41), (28), (27), (25), (14), (14), (8), (4), (3), (3), (2), (2), (1)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Purification Affinity purification
Immunogen Recombinant protein of human IRF3
Isotype IgG

Target Details

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Antigen
Alternative Name IRF3 (IRF3 Antibody Abstract)
Background Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3gamma/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E). IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal Ser and Thr residues (between 385 and 405), leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity. During infection, IRF-3 is likely activated through a pathway that includes activation of Toll-like receptors and a kinase complex that includes IKKepsilon and TBK1. IRF-3 is phosphorylated at Ser396 following viral infection, expression of viral nucleocapsid, and double-stranded RNA treatment. These events likely play a role in activation of IRF-3.
Synonyms: IRF-3, IRF3
Gene ID 3661
UniProt Q14653
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Application Details

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Application Notes Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
Restrictions For Research Use only

Handling

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Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze / thaw cycles
Storage -20 °C/-80 °C

References

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Product cited in:

Zhong, Wang, Fang, Zhang, Luo, Shang, Ouyang, Ouyang, Chen, Xiao: "Ubiquitin-specific proteases 25 negatively regulates virus-induced type I interferon signaling." in: PLoS ONE, Vol. 8, Issue 11, pp. e80976, 2013

Images

Product details Target Details Application Details Handling References back to top
Supplier Images
Western Blotting (WB) image for anti-Interferon Regulatory Factor 3 (IRF3) antibody (ABIN3023115) Western blot analysis of extracts of various cell lines, using IRF3 antibody.
Immunofluorescence (IF) image for anti-Interferon Regulatory Factor 3 (IRF3) antibody (ABIN3023115) Immunofluorescence analysis of U2OS cell using IRF3 antibody. Blue: DAPI for nuclear ...