anti-Human TICAM1 antibody for Western Blotting

Recommended TICAM1 Antibody (supplied by: Log in to see )

Antigen
Toll-Like Receptor Adaptor Molecule 1 (TICAM1) Antibodies
  • IIAE6
  • MyD88-3
  • PRVTIRB
  • TICAM-1
  • TRIF
  • AW046014
  • AW547018
  • trif
  • toll like receptor adaptor molecule 1
  • toll-like receptor adaptor molecule 1
  • TICAM1
  • Ticam1
  • ticam1
Epitope
Internal Region
25
6
6
4
3
3
2
2
2
2
2
2
2
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
84
31
14
4
3
1
1
Host
Rabbit
78
8
8
Clonality
Polyclonal
Conjugate
This TICAM1 antibody is un-conjugated
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
86
32
18
18
13
7
7
4
4
1
1
1
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Catalog No. ABIN266066
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19.563524 ABIN2855929 ICC IF WB Rabbit IgG C-Term Log in to see Polyclonal 0
19.563524 ABIN3021572 IHC WB Rabbit IgG Log in to see Polyclonal 0
17.311565 ABIN1169062 IP WB Rabbit AA 4-31, N-Term Log in to see Polyclonal 3
16.751959 ABIN1871543 ICC IHC WB Rabbit IgG AA 443-685 Log in to see Polyclonal 0
16.751959 ABIN1875095 IHC WB Rabbit IgG Log in to see Polyclonal 0
16.751959 ABIN2903154 IF/ICC IHC IP WB Rabbit AA 443-685 Log in to see Polyclonal 0
15.251959 ABIN3044491 IHC (fro) IHC (p) WB Rabbit IgG AA 692-712, C-Term Log in to see Polyclonal 1
13.751959 ABIN6265566 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
12.811565 ABIN2613488 ELISA WB Goat Internal Region Log in to see Polyclonal 0
12.811565 ABIN4902391 ELISA WB Rabbit IgG AA 663-712 Log in to see Polyclonal 0
12.811565 ABIN6149149 WB Rabbit Log in to see Polyclonal 0
12.251959 ABIN541214 IHC (p) WB Rabbit Log in to see Polyclonal 3
10.751959 ABIN3029185 IHC (fro) IHC (p) WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 0
10 ABIN213993 IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
10 ABIN6292306 IHC WB Rabbit IgG Log in to see Polyclonal 0
9.251959 ABIN1881885 WB Rabbit AA 115-143, N-Term Log in to see Polyclonal 5
9.251959 ABIN537469 WB Rabbit Log in to see Polyclonal 3
7.751959 ABIN3223003 IHC WB Rabbit C-Term Log in to see Polyclonal 0
7.751959 ABIN1030771 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 0
7 ABIN457458 IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0

General

Antigen Toll-Like Receptor Adaptor Molecule 1 (TICAM1) Antibodies
Epitope Internal Region
(25), (6), (6), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Reactivity Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(84), (31), (14), (4), (3), (1), (1)
Host Rabbit
(78), (8), (8)
Clonality
Polyclonal
Conjugate This TICAM1 antibody is un-conjugated
(1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(86), (32), (18), (18), (13), (7), (7), (4), (4), (1), (1), (1)
Pubmed 26 references available
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Product Details anti-TICAM1 Antibody

Target Details TICAM1 Application Details Handling References for anti-TICAM1 antibody (ABIN266066) Images
Specificity The will cross-react with human TRIF, but additional bands may be seen.
Purification Unpurified
Immunogen Synthetic peptide made to an internal portion of the mouse TRIF protein (between amino acids 100-180) [UniProt Q80UF7].
Plasmids, Primers & others

Target Details TICAM1

Product Details anti-TICAM1 Antibody Application Details Handling References for anti-TICAM1 antibody (ABIN266066) Images back to top
Antigen
Alternative Name TRIF/TICAM1 (TICAM1 Antibody Abstract)
Background Gene Symbol: TICAM1
Molecular Weight Theoretical MW: 76 kDa
Gene ID 148022
UniProt Q80UF7
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-TICAM1 Antibody Target Details TICAM1 Handling References for anti-TICAM1 antibody (ABIN266066) Images back to top
Application Notes Western Blot 1:500-1:1000, Simple Western 1:100, Immunocytochemistry/Immunofluorescence 1:10-1:100, ImmunoprecipitationThe approx. 70 kD band detected by this antibody in western blot, corresponding to TRIF protein, is blocked by the immunizing peptide. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunoprecipitation reported in scientific literature (PMID 28007523).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-TICAM1 Antibody Target Details TICAM1 Application Details References for anti-TICAM1 antibody (ABIN266066) Images back to top
Format Liquid
Buffer Whole antisera
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-TICAM1 antibody (ABIN266066)

Product Details anti-TICAM1 Antibody Target Details TICAM1 Application Details Handling Images back to top
Product cited in:

Kim, Beon, Lee, Park, Ahn, Kim, Park, Kim, Yuk, Kang, Lee, Jo, Seong, Kim: "Inositol polyphosphate multikinase promotes Toll-like receptor-induced inflammation by stabilizing TRAF6." in: Science advances, Vol. 3, Issue 4, pp. e1602296, 2017 Method employed by authors: Immunoprecipitation (IP) (Sample species: Mouse (Murine)).

Popiolek-Barczyk, Piotrowska, Makuch, Mika: "Biphalin, a Dimeric Enkephalin, Alleviates LPS-Induced Activation in Rat Primary Microglial Cultures in Opioid Receptor-Dependent and Receptor-Independent Manners." in: Neural plasticity, Vol. 2017, pp. 3829472, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Zhang, Xiang, Wang, Yan, Zhu, Guo, Sengupta, Chen, Loughran, Lu, Wang, Billiar et al.: "Interferon β (IFN-β) Production during the Double-stranded RNA (dsRNA) Response in Hepatocytes Involves Coordinated and Feedforward Signaling through Toll-like Receptor 3 (TLR3), RNA-dependent ..." in: The Journal of biological chemistry, Vol. 291, Issue 29, pp. 15093-107, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Hu, Cong, Chen, Qi, Wu, Zhou, Yoo, Li, Sun, Wu, Zhao, Chen, Yu, Du, Wang: "Synergy of TLR3 and 7 ligands significantly enhances function of DCs to present inactivated PRRSV antigen through TRIF/MyD88-NF-κB signaling pathway." in: Scientific reports, Vol. 6, pp. 23977, 2016 (Sample species: Pig (Porcine)). Further details: Western Blotting

Lan, Han, Li, Li, Gao, Cheng, Wan, Zhu, Wang: "Pinocembrin protects hemorrhagic brain primarily by inhibiting toll-like receptor 4 and reducing M1 phenotype microglia." in: Brain, behavior, and immunity, Vol. 61, pp. 326-339, 2016 Method employed by authors: Immunoprecipitation (IP) (Sample species: Mouse (Murine)).

Ma, Yu, Xie, Sun, Lin, Ding, Luo, Cai: "Toll-Like Receptors Promote Mitochondrial Translocation of Nuclear Transcription Factor Nuclear Factor of Activated T-Cells in Prolonged Microglial Activation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 30, pp. 10799-814, 2015 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Haque, Koide, Odkhuu, Tsolmongyn, Naiki, Komatsu, Yoshida, Yokochi: "Mouse pyrin and HIN domain family member 1 (pyhin1) protein positively regulates LPS-induced IFN-β and NO production in macrophages." in: Innate immunity, Vol. 20, Issue 1, pp. 40-8, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting

Vogt, Scull, Friling, Horwitz, Donovan, Dorner, Gerold, Labitt, Rice, Ploss: "Recapitulation of the hepatitis C virus life-cycle in engineered murine cell lines." in: Virology, Vol. 444, Issue 1-2, pp. 1-11, 2013 Further details: Western Blotting

Abe, Hirasaka, Kagawa, Kohno, Ochi, Utsunomiya, Sakai, Ohno, Teshima-Kondo, Okumura, Oarada, Maekawa, Terao, Mills, Nikawa: "Cbl-b is a critical regulator of macrophage activation associated with obesity-induced insulin resistance in mice." in: Diabetes, Vol. 62, Issue 6, pp. 1957-69, 2013

Tian, Guo, Liu, Liu, Weng, Dong, Knowlton, Yuan, Lin: "Extracellular HSP60 induces inflammation through activating and up-regulating TLRs in cardiomyocytes." in: Cardiovascular research, Vol. 98, Issue 3, pp. 391-401, 2013 (Sample species: Rat (Rattus)). Further details: Western Blotting

Seleme, Lei, Burg, Goh, Metz, Steele, Tse: "Dysregulated TLR3-dependent signaling and innate immune activation in superoxide-deficient macrophages from nonobese diabetic mice." in: Free radical biology & medicine, Vol. 52, Issue 9, pp. 2047-56, 2012 Further details: Western Blotting

De Luca, Iannitti, Bozza, Beau, Casagrande, DAngelo, Moretti, Cunha, Giovannini, Massi-Benedetti, Carvalho, Boon, Latgé, Romani: "CD4(+) T cell vaccination overcomes defective cross-presentation of fungal antigens in a mouse model of chronic granulomatous disease." in: The Journal of clinical investigation, Vol. 122, Issue 5, pp. 1816-31, 2012 Further details: Western Blotting

Zhang, Kim, Bao, Facchinetti, Jung, Ghaffari, Qin, Cheng, Liu: "DDX1, DDX21, and DHX36 helicases form a complex with the adaptor molecule TRIF to sense dsRNA in dendritic cells." in: Immunity, Vol. 34, Issue 6, pp. 866-78, 2011

Shi, Kehrl: "TRAF6 and A20 regulate lysine 63-linked ubiquitination of Beclin-1 to control TLR4-induced autophagy." in: Science signaling, Vol. 3, Issue 123, pp. ra42, 2010 Further details: Western Blotting

Kong, Thimmulappa, Kombairaju, Biswal: "NADPH oxidase-dependent reactive oxygen species mediate amplified TLR4 signaling and sepsis-induced mortality in Nrf2-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 1, pp. 569-77, 2010 (Sample species: Mouse (Murine)). Further details: Western Blotting

Wang, Kim, Nakahira, Ryter, Choi: "The heme oxygenase-1/carbon monoxide pathway suppresses TLR4 signaling by regulating the interaction of TLR4 with caveolin-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 6, pp. 3809-18, 2009 Further details: Western Blotting,Immunoprecipitation

Dasu, Park, Devaraj, Jialal: "Pioglitazone inhibits Toll-like receptor expression and activity in human monocytes and db/db mice." in: Endocrinology, Vol. 150, Issue 8, pp. 3457-64, 2009 Further details: Western Blotting

Wong, Kwon, Choi, Kim, Nakahira, Hwang: "Fatty acids modulate Toll-like receptor 4 activation through regulation of receptor dimerization and recruitment into lipid rafts in a reactive oxygen species-dependent manner." in: The Journal of biological chemistry, Vol. 284, Issue 40, pp. 27384-92, 2009 Further details: Western Blotting

Shi, Kehrl: "MyD88 and Trif target Beclin 1 to trigger autophagy in macrophages." in: The Journal of biological chemistry, Vol. 283, Issue 48, pp. 33175-82, 2008 Further details: Western Blotting

Krikun, Lockwood, Abrahams, Mor, Paidas, Guller: "Expression of Toll-like receptors in the human decidua." in: Histology and histopathology, Vol. 22, Issue 8, pp. 847-54, 2007 (Sample species: Human). Further details: Western Blotting

Images

Product Details anti-TICAM1 Antibody Target Details TICAM1 Application Details Handling References for anti-TICAM1 antibody (ABIN266066) back to top
Supplier Images
Western Blotting (WB) image for anti-Toll-Like Receptor Adaptor Molecule 1 (TICAM1) (Internal Region) antibody (ABIN266066) Western blot analysis of TICAM in mouse spleen lysate using ABIN266066 at 1:1000 dilu...
Simple Western (SimWes) image for anti-Toll-Like Receptor Adaptor Molecule 1 (TICAM1) (Internal Region) antibody (ABIN266066) Simple Western: TRIF/TICAM1 Antibody [ABIN2660660] - Simple Western lane view shows a...
Western Blotting (WB) image for anti-Toll-Like Receptor Adaptor Molecule 1 (TICAM1) (Internal Region) antibody (ABIN266066) Western Blot: TRIF/TICAM1 Antibody [ABIN2660660] - Analysis of TRIF in mouse A) intes...
Western Blotting (WB) image for anti-Toll-Like Receptor Adaptor Molecule 1 (TICAM1) (Internal Region) antibody (ABIN266066) Western Blot: TRIF/TICAM1 Antibody [ABIN2660660] - Analysis of TRIF in mouse spleen p...
Immunofluorescence (IF) image for anti-Toll-Like Receptor Adaptor Molecule 1 (TICAM1) (Internal Region) antibody (ABIN266066) Immunocytochemistry/Immunofluorescence: TRIF/TICAM1 Antibody [ABIN2660660] - TRIF ant...