anti-Human TLR4 antibody for Immunohistochemistry

Recommended TLR4 Antibody (supplied by: Log in to see )

Antigen
Toll-Like Receptor 4 (TLR4) Antibodies
  • ARMD10
  • CD284
  • TLR-4
  • TOLL
  • Lps
  • Ly87
  • Ran/M1
  • Rasl2-8
  • toll like receptor 4
  • toll-like receptor 4
  • TLR4
  • Tlr4
Reactivity
Human, Mouse (Murine), Rat (Rattus)
564
271
173
93
71
71
63
26
12
9
4
2
1
1
Host
Mouse
349
273
46
8
Clonality
Monoclonal
Conjugate
This TLR4 antibody is un-conjugated
49
47
45
23
15
14
13
10
8
8
8
6
6
6
6
6
6
6
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), In vitro Assay (in vitro), Western Blotting (WB)
394
352
189
134
129
121
102
92
34
32
27
26
26
21
20
15
13
8
7
4
4
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN252522
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
29.46781 ABIN4899843 CyTOF FACS ICC IHC Neut WB Goat IgG AA 24-631 Log in to see Polyclonal 14
27.77914 ABIN2485970 ICC FACS IF IHC WB APC Rabbit AA 420-435 Log in to see Polyclonal 6
27.77914 ABIN2485968 ICC FACS IF IHC WB Atto 700 Rabbit AA 420-435 Log in to see Polyclonal 6
27.77914 ABIN2485972 ICC FACS IF IHC WB FITC Rabbit AA 420-435 Log in to see Polyclonal 6
27.77914 ABIN2485964 ICC FACS IF IHC WB Atto 594 Rabbit AA 420-435 Log in to see Polyclonal 6
27.77914 ABIN2485973 ICC FACS IF IHC WB HRP Rabbit AA 420-435 Log in to see Polyclonal 6
27.77914 ABIN2485974 ICC FACS IF IHC WB PE-Atto 594 Rabbit AA 420-435 Log in to see Polyclonal 6
27.77914 ABIN2485975 ICC FACS IF IHC WB PerCP Rabbit AA 420-435 Log in to see Polyclonal 6
27.77914 ABIN2485976 ICC FACS IF IHC WB PE Rabbit AA 420-435 Log in to see Polyclonal 6
27.77914 ABIN2485967 ICC FACS IF IHC WB Atto 680 Rabbit AA 420-435 Log in to see Polyclonal 6
27.77914 ABIN2485977 ICC FACS IF IHC WB Streptavidin Rabbit AA 420-435 Log in to see Polyclonal 6
27.77914 ABIN2485969 ICC FACS IF IHC WB Alkaline Phosphatase (AP) Rabbit AA 420-435 Log in to see Polyclonal 6
27.77914 ABIN2485971 ICC FACS IF IHC WB Biotin Rabbit AA 420-435 Log in to see Polyclonal 6
27.77914 ABIN2485961 ICC FACS IF IHC WB Atto 390 Rabbit AA 420-435 Log in to see Polyclonal 6
27.77914 ABIN2485963 ICC FACS IF IHC WB Atto 565 Rabbit AA 420-435 Log in to see Polyclonal 6
27.77914 ABIN2485965 ICC FACS IF IHC WB Atto 633 Rabbit AA 420-435 Log in to see Polyclonal 6
27.77914 ABIN2485962 ICC FACS IF IHC WB Atto 488 Rabbit AA 420-435 Log in to see Polyclonal 6
27.77914 ABIN2485966 ICC FACS IF IHC WB Atto 655 Rabbit AA 420-435 Log in to see Polyclonal 6
23.311333 ABIN361724 ICC FACS IF IHC WB Rabbit AA 420-435 Log in to see Polyclonal 9
21.96781 ABIN6270322 ICC IF IHC WB Rabbit IgG pSer800 Log in to see Polyclonal 0

General

Antigen Toll-Like Receptor 4 (TLR4) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(564), (271), (173), (93), (71), (71), (63), (26), (12), (9), (4), (2), (1), (1)
Host Mouse
(349), (273), (46), (8)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TLR4 antibody is un-conjugated
(49), (47), (45), (23), (15), (14), (13), (10), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Chromatin Immunoprecipitation (ChIP), Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), In vitro Assay (in vitro), Western Blotting (WB)
(394), (352), (189), (134), (129), (121), (102), (92), (34), (32), (27), (26), (26), (21), (20), (15), (13), (8), (7), (4), (4), (3), (2), (1), (1), (1), (1), (1)
Pubmed 72 references available
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Product Details anti-TLR4 Antibody

Target Details TLR4 Application Details Handling References for anti-TLR4 antibody (ABIN252522) Images
Purification Protein G purified
Immunogen This antibody was developed against a portion of amino acids 100-200 of human TLR4 (NP_612564).
Clone 76B357-1
Isotype IgG2b kappa
Plasmids, Primers & others

Target Details TLR4

Product Details anti-TLR4 Antibody Application Details Handling References for anti-TLR4 antibody (ABIN252522) Images back to top
Antigen
Alternative Name TLR4 (TLR4 Antibody Abstract)
Background Gene Symbol: TLR4
Gene ID 7099
UniProt O00206
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades

Application Details

Product Details anti-TLR4 Antibody Target Details TLR4 Handling References for anti-TLR4 antibody (ABIN252522) Images back to top
Application Notes Western Blot 1-3 μg/mL, Flow Cytometry, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 5 μg/mL, Immunohistochemistry-Frozen 1:500, In vitro assay, Flow (Cell Surface), Flow (Intracellular), Chromatin Immunoprecipitation (ChIP), CyTOF-readyFlow (Intracellular) use reported in literature (See Cohen et al, 2008), Immunofluorescence (See Nowicki et al, 2009), IHC-F (Nowicki et al, 2012), Flow (cell surface) use reported in scientific literature (PMID 23796194). Use in chromatin immunoprecipitation reported in scientific literature (PMID 21966468). Use In vitro reported in scientific literature (PMID 26446256).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-TLR4 Antibody Target Details TLR4 Application Details References for anti-TLR4 antibody (ABIN252522) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-TLR4 antibody (ABIN252522)

Product Details anti-TLR4 Antibody Target Details TLR4 Application Details Handling Images back to top
Product cited in:

Chen, Yan, Guo: "Inhibitory effect of Patrinia on BRL-3A cell apoptosis through the TLR4/PI3K/AKT/GSK3β and TLR4/P38/JNK signaling pathways." in: Molecular medicine reports, Vol. 17, Issue 4, pp. 5344-5349, 2018

Bhattacharjee, Keyel: "Cholesterol-dependent cytolysins impair pro-inflammatory macrophage responses." in: Scientific reports, Vol. 8, Issue 1, pp. 6458, 2018

Domon, Nagai, Maekawa, Oda, Yonezawa, Takeda, Hiyoshi, Tamura, Yamaguchi, Kawabata, Terao: "Neutrophil Elastase Subverts the Immune Response by Cleaving Toll-Like Receptors and Cytokines in Pneumococcal Pneumonia." in: Frontiers in immunology, Vol. 9, pp. 732, 2018

Sun, Li, Ma, Zhang, Song, Lv, He: "Inhibiting High Mobility Group Box-1 Reduces Early Spinal Cord Edema and Attenuates Astrocyte Activation and Aquaporin-4 Expression after Spinal Cord Injury in Rats." in: Journal of neurotrauma, 2018

Bernardini, Zannoni, Bertocchi, Tubon, Fernandez, Forni: "Water/ethanol extract of Cucumis sativus L. fruit attenuates lipopolysaccharide-induced inflammatory response in endothelial cells." in: BMC complementary and alternative medicine, Vol. 18, Issue 1, pp. 194, 2018

Bidne, Kvidera, Ross, Baumgard, Keating: "Impact of repeated lipopolysaccharide administration on ovarian signaling during the follicular phase of the estrous cycle in post-pubertal pigs." in: Journal of animal science, 2018

Choi, Kang, Song, Kim, Chung, Ylaya, Kim, Koh, Chung, Kim, Hewitt: "Elevated expression of pancreatic adenocarcinoma upregulated factor (PAUF) is associated with poor prognosis and chemoresistance in epithelial ovarian cancer." in: Scientific reports, Vol. 8, Issue 1, pp. 12161, 2018

Mukherjee, Hough, Chattopadhyay, Grijalva, OConnor, Meriwether, Wagner, Ntambi, Navab, Reddy, Fogelman: "Role of enterocyte stearoyl-Co-A desaturase-1 in LDLR-null mice." in: Journal of lipid research, Vol. 59, Issue 10, pp. 1818-1840, 2018

Tobita, Watanabe, Saito et al.: "Specific vaginal lactobacilli suppress the inflammation induced by lipopolysaccharide stimulation through downregulation of toll-like receptor 4 expression in human embryonic intestinal epithelial ..." in: Bioscience of microbiota, food and health, Vol. 36, Issue 1, pp. 39-44, 2017 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Azuma, Taniguchi, Nakamura, Nagira, Khine, Kiyama, Uegaki, Izawa, Harada: "Lipopolysaccharide promotes the development of murine endometriosis-like lesions via the nuclear factor-kappa B pathway." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 77, Issue 4, 2017 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Nakano, Shimazawa, Ojino, Izawa, Takeuchi, Inoue, Tsuruma, Hara: "Toll-like receptor 4 inhibitor protects against retinal ganglion cell damage induced by optic nerve crush in mice." in: Journal of pharmacological sciences, Vol. 133, Issue 3, pp. 176-183, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Camps-Bossacoma, Pérez-Cano, Franch, Untersmayr, Castell: "Effect of a cocoa diet on the small intestine and gut-associated lymphoid tissue composition in an oral sensitization model in rats." in: The Journal of nutritional biochemistry, Vol. 42, pp. 182-193, 2017

Schöniger, Gräfe, Schoon: "Expression of Toll-like receptors 2, 4 and 6 in different cell populations of the equine endometrium." in: Veterinary immunology and immunopathology, Vol. 185, pp. 7-13, 2017

Szasz, Wenceslau, Burgess, Nunes, Webb: "Toll-Like Receptor 4 Activation Contributes to Diabetic Bladder Dysfunction in a Murine Model of Type 1 Diabetes." in: Diabetes, Vol. 65, Issue 12, pp. 3754-3764, 2017

Liu, Wang, Cao, Wang, Liu, Gao, Hu, Yuan, Lin: "Up-regulated TLR4 in cardiomyocytes exacerbates heart failure after long-term myocardial infarction." in: Journal of cellular and molecular medicine, Vol. 19, Issue 12, pp. 2728-40, 2016 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Western Blotting,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence

Mishima, Ishihara, Oka, Fukuba, Oshima, Sonoyama, Yamashita, Tada, Kusunoki, Moriyama, Yuki, Kawashima, Kinoshita: "Decreased Frequency of Intestinal Regulatory CD5+ B Cells in Colonic Inflammation." in: PLoS ONE, Vol. 11, Issue 1, pp. e0146191, 2016 (Sample species: Mouse (Murine)). Further details: Flow Cytometry

Okada, Arai, Itoh, Adachi, Hayashida, Nakase, Ikemoto: "CD68 on rat macrophages binds tightly to S100A8 and S100A9 and helps to regulate the cells' immune functions." in: Journal of leukocyte biology, Vol. 100, Issue 5, pp. 1093-1104, 2016

Li, Bao, Leung, Ma, Zhang, Lu, Wang, Han: "Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour." in: Molecules (Basel, Switzerland), Vol. 21, Issue 6, 2016

Kuramochi, Izawa, Pervin, Bondoc, Kuwamura, Yamate: "The kinetics of damage-associated molecular patterns (DAMPs) and toll-like receptors during thioacetamide-induced acute liver injury in rats." in: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, Vol. 68, Issue 8, pp. 471-7, 2016 (Sample species: Rat (Rattus)). Further details: Western Blotting

Burgueño, Barba, Eyre, Romero, Neunlist, Fernández: "TLR2 and TLR9 modulate enteric nervous system inflammatory responses to lipopolysaccharide." in: Journal of neuroinflammation, Vol. 13, Issue 1, pp. 187, 2016 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Images

Product Details anti-TLR4 Antibody Target Details TLR4 Application Details Handling References for anti-TLR4 antibody (ABIN252522) back to top
Supplier Images
Western Blotting (WB) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Western Blot: TLR4 Antibody [ABIN252522] - Western blot analysis of TLR4 using ABIN25...
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Flow Cytometry: TLR4 Antibody [ABIN252522] - Flow analysis of TLR4 in 10^6 ThP1 cells...
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Flow Cytometry: TLR4 Antibody (76B357.1) [ABIN2525226] - Analysis of FITC conjugate o...
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Flow Cytometry: TLR4 Antibody (76B357.1) [ABIN2525226] - Surface staining of mouse sp...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [ABIN2525226] - Formalin-fixe...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [ABIN2525226] - Analysis usin...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [ABIN2525226] - Analysis of f...
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Flow Cytometry: TLR4 Antibody (76B357.1) [ABIN2525226] - Analysis using Azide Free ve...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [ABIN2525226] - Analysis of f...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [ABIN2525226] - Analysis of f...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [ABIN2525226] - Analysis of f...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [ABIN2525226] - Analysis of R...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [ABIN2525226] - Analysis of T...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [ABIN2525226] - Formalin-fixe...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [ABIN2525226] - Analysis usin...
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Flow Cytometry: TLR4 Antibody (76B357.1) - Flow cytometric analysis of formaldehyde ...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Immunohistochemistry-Frozen: TLR4 Antibody (76B357.1) - This image is TLR4(green) an...
 image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Flow (Intracellular): TLR4 Antibody (76B357.1) - An intracellular stain was performe...
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Flow (Intracellular): TLR4 Antibody (76B357.1) - Flow Cytometry: TLR4 Antibody (76B3...
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN252522) Flow Cytometry: TLR4 Antibody (76B357.1) - An intracellular stain was performed on J...