anti-Human TLR4 antibody for Immunohistochemistry

Recommended TLR4 Antibody (supplied by: Log in to see )

Antigen
Toll-Like Receptor 4 (TLR4) Antibodies
  • ARMD10
  • CD284
  • TLR-4
  • TOLL
  • Lps
  • Ly87
  • Ran/M1
  • Rasl2-8
  • toll like receptor 4
  • toll-like receptor 4
  • TLR4
  • Tlr4
Epitope
AA 24-631
59
30
28
25
16
16
15
15
14
13
11
9
8
7
7
6
5
5
5
5
5
5
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
555
275
174
94
72
72
64
27
12
9
4
2
1
1
Host
Goat
340
273
47
6
Clonality
Polyclonal
Conjugate
This TLR4 antibody is un-conjugated
49
47
47
23
15
14
13
10
9
9
8
6
6
6
6
6
6
6
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
392
340
185
137
130
123
98
92
34
30
27
26
26
21
20
16
13
8
7
4
4
3
2
1
1
1
Supplier
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Catalog No. ABIN4899843
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
27.545004 ABIN2485968 ICC FACS IF IHC WB Atto 700 Rabbit AA 420-435 Log in to see Polyclonal 6
27.545004 ABIN2485962 ICC FACS IF IHC WB Atto 488 Rabbit AA 420-435 Log in to see Polyclonal 6
27.545004 ABIN2485964 ICC FACS IF IHC WB Atto 594 Rabbit AA 420-435 Log in to see Polyclonal 6
27.545004 ABIN2485963 ICC FACS IF IHC WB Atto 565 Rabbit AA 420-435 Log in to see Polyclonal 6
27.545004 ABIN2485961 ICC FACS IF IHC WB Atto 390 Rabbit AA 420-435 Log in to see Polyclonal 6
27.545004 ABIN2485965 ICC FACS IF IHC WB Atto 633 Rabbit AA 420-435 Log in to see Polyclonal 6
27.545004 ABIN2485967 ICC FACS IF IHC WB Atto 680 Rabbit AA 420-435 Log in to see Polyclonal 6
27.545004 ABIN2485970 ICC FACS IF IHC WB APC Rabbit AA 420-435 Log in to see Polyclonal 6
27.545004 ABIN2485971 ICC FACS IF IHC WB Biotin Rabbit AA 420-435 Log in to see Polyclonal 6
27.545004 ABIN2485972 ICC FACS IF IHC WB FITC Rabbit AA 420-435 Log in to see Polyclonal 6
27.545004 ABIN2485973 ICC FACS IF IHC WB HRP Rabbit AA 420-435 Log in to see Polyclonal 6
27.545004 ABIN2485974 ICC FACS IF IHC WB PE-Atto 594 Rabbit AA 420-435 Log in to see Polyclonal 6
27.545004 ABIN2485976 ICC FACS IF IHC WB PE Rabbit AA 420-435 Log in to see Polyclonal 6
27.545004 ABIN2485975 ICC FACS IF IHC WB PerCP Rabbit AA 420-435 Log in to see Polyclonal 6
27.545004 ABIN2485977 ICC FACS IF IHC WB Streptavidin Rabbit AA 420-435 Log in to see Polyclonal 6
27.545004 ABIN2485966 ICC FACS IF IHC WB Atto 655 Rabbit AA 420-435 Log in to see Polyclonal 6
27.545004 ABIN2485969 ICC FACS IF IHC WB Alkaline Phosphatase (AP) Rabbit AA 420-435 Log in to see Polyclonal 6
23.07224 ABIN361724 ICC FACS IF IHC WB Rabbit AA 420-435 Log in to see Polyclonal 9
21.972763 ABIN6270322 ICC IF IHC WB Rabbit IgG pSer800 Log in to see Polyclonal 0
18.57224 ABIN3183753 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0

General

Antigen Toll-Like Receptor 4 (TLR4) Antibodies
Epitope AA 24-631
(59), (30), (28), (25), (16), (16), (15), (15), (14), (13), (11), (9), (8), (7), (7), (6), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(555), (275), (174), (94), (72), (72), (64), (27), (12), (9), (4), (2), (1), (1)
Host Goat
(340), (273), (47), (6)
Clonality
Polyclonal
Conjugate This TLR4 antibody is un-conjugated
(49), (47), (47), (23), (15), (14), (13), (10), (9), (9), (8), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
(392), (340), (185), (137), (130), (123), (98), (92), (34), (30), (27), (26), (26), (21), (20), (16), (13), (8), (7), (4), (4), (3), (2), (1), (1), (1)
Pubmed 14 references available
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Product Details anti-TLR4 Antibody

Target Details TLR4 Application Details Handling References for anti-TLR4 antibody (ABIN4899843) Images
Specificity Detects human TLR4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30 % cross-reactivity with recombinant mouse (rm) TLR4 is observed and less than 5 % cross-reactivity with recombinant human (rh) TLR1, rhTLR2, rhTLR3 and rmTLR6 is observed.
Purification Immunogen affinity purified
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen Mouse myeloma cell line NS0-derived recombinant human TLR4, Glu24-Lys631, Accession # O00206
Isotype IgG
Plasmids, Primers & others

Target Details TLR4

Product Details anti-TLR4 Antibody Application Details Handling References for anti-TLR4 antibody (ABIN4899843) Images back to top
Antigen
Alternative Name TLR4 (TLR4 Antibody Abstract)
Background Alternative Names: ARMD10, CD284, CD284 antigen, hTollhomolog of Drosophila toll, TOLL, toll-like receptor 4
Gene ID 7099
UniProt O00206
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades

Application Details

Product Details anti-TLR4 Antibody Target Details TLR4 Handling References for anti-TLR4 antibody (ABIN4899843) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Immunohistochemistry 5-15 μg/mL
Neutralization 1.5-7.5 μg/mL
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-TLR4 Antibody Target Details TLR4 Application Details References for anti-TLR4 antibody (ABIN4899843) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-TLR4 antibody (ABIN4899843)

Product Details anti-TLR4 Antibody Target Details TLR4 Application Details Handling Images back to top
Product cited in:

Sootichote, Thuwajit, Singsuksawat, Warnnissorn, Yenchitsomanus, Ithimakin, Chantharasamee, Thuwajit: "Compound A attenuates toll-like receptor 4-mediated paclitaxel resistance in breast cancer and melanoma through suppression of IL-8." in: BMC cancer, Vol. 18, Issue 1, pp. 231, 2018 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Human).

Sandri, Urban Borbely, Fernandes, de Oliveira, Knebel, Ruano, Zugaib, Filippin-Monteiro, Bevilacqua, Campa: "Serum amyloid A in the placenta and its role in trophoblast invasion." in: PLoS ONE, Vol. 9, Issue 3, pp. e90881, 2016 Method employed by authors: Functional Studies (Func), Functional Studies (Func) (Sample species: Human).

Ben Haij, Planès, Leghmari, Serrero, Delobel, Izopet, BenMohamed, Bahraoui: "HIV-1 Tat Protein Induces Production of Proinflammatory Cytokines by Human Dendritic Cells and Monocytes/Macrophages through Engagement of TLR4-MD2-CD14 Complex and Activation of NF-κB Pathway." in: PLoS ONE, Vol. 10, Issue 6, pp. e0129425, 2015 Method employed by authors: Flow Cytometry (FACS), Flow Cytometry (FACS), Flow Cytometry (FACS) (Sample species: Human).

Avlas, Bragg, Fuks, Nicholson, Farkash, Porat, Aravot, Levy-Drummer, Cohen, Shainberg, Arad, Hochhauser: "TLR4 Expression Is Associated with Left Ventricular Dysfunction in Patients Undergoing Coronary Artery Bypass Surgery." in: PLoS ONE, Vol. 10, Issue 6, pp. e0120175, 2015 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Human).

Su, Yang, Lee, Sheu, Hsu, Lin, Chow: "The C-terminal disulfide bonds of Helicobacter pylori GroES are critical for IL-8 secretion via the TLR4-dependent pathway in gastric epithelial cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 8, pp. 3997-4007, 2015 Method employed by authors: Immunoprecipitation (IP), Immunoprecipitation (IP), Immunoprecipitation (IP) (Sample species: Helicobacter pylori).

Vogel, Börger, Peters, Förster, Liebfried, Metzger, Meisel, Däubener, Trapp, Fischer, Gawaz, Sorg: "Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death." in: Cell death and differentiation, Vol. 22, Issue 7, pp. 1219-30, 2015 Method employed by authors: Neutralization (Neut), Neutralization (Neut), Neutralization (Neut) (Sample species: Mouse (Murine)).

Kelsh, You, Horzempa, Zheng, McKeown-Longo: "Regulation of the innate immune response by fibronectin: synergism between the III-1 and EDA domains." in: PLoS ONE, Vol. 9, Issue 7, pp. e102974, 2015 Method employed by authors: Neutralization (Neut), Neutralization (Neut) (Sample species: Human).

Alizadeh, Tripathi, Abdi, Smith: "Pathogenic strains of Acanthamoeba are recognized by TLR4 and initiated inflammatory responses in the cornea." in: PLoS ONE, Vol. 9, Issue 3, pp. e92375, 2014 Method employed by authors: Neutralization (Neut), Neutralization (Neut), Neutralization (Neut) (Sample species: Human).

Vogel, Chatterjee, Metzger, Borst, Geisler, Seizer, Müller, Mack, Schumann, Bhring, Lang, Sorg, Langer, Gawaz et al.: "Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth ..." in: The Journal of biological chemistry, Vol. 289, Issue 16, pp. 11068-82, 2014 Method employed by authors: Flow Cytometry (FACS), Flow Cytometry (FACS) (Sample species: Human).

Mogami, Keller, Shi, Word: "Effect of thrombin on human amnion mesenchymal cells, mouse fetal membranes, and preterm birth." in: The Journal of biological chemistry, Vol. 289, Issue 19, pp. 13295-307, 2014 Method employed by authors: Neutralization (Neut), Neutralization (Neut) (Sample species: Human).

Mogami, Kishore, Shi, Keller, Akgul, Word: "Fetal fibronectin signaling induces matrix metalloproteases and cyclooxygenase-2 (COX-2) in amnion cells and preterm birth in mice." in: The Journal of biological chemistry, Vol. 288, Issue 3, pp. 1953-66, 2013 Method employed by authors: Neutralization (Neut), Neutralization (Neut), Neutralization (Neut) (Sample species: Human).

Zhao, Anand, Ganju: "Slit2-Robo4 pathway modulates lipopolysaccharide-induced endothelial inflammation and its expression is dysregulated during endotoxemia." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 1, pp. 385-93, 2013 Method employed by authors: Neutralization (Neut), Neutralization (Neut), Neutralization (Neut) (Sample species: Human).

Lumsden, Nelson, Dispenza, Gilliland, Cong, Zaenglein, Thiboutot: "Isotretinoin increases skin-surface levels of neutrophil gelatinase-associated lipocalin in patients treated for severe acne." in: The British journal of dermatology, Vol. 165, Issue 2, pp. 302-10, 2011 Method employed by authors: Neutralization (Neut), Neutralization (Neut), Neutralization (Neut) (Sample species: Human).

Zunt, Burton, Goldblatt, Dobbins, Srinivasan: "Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells." in: Clinical and experimental immunology, Vol. 156, Issue 2, pp. 285-93, 2009 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-TLR4 Antibody Target Details TLR4 Application Details Handling References for anti-TLR4 antibody (ABIN4899843) back to top
Supplier Images
Western Blotting (WB) image for anti-Toll-Like Receptor 4 (TLR4) (AA 24-631) antibody (ABIN4899843) Detection of Human TLR4 by Western Blot. Western blot shows lysates of human placenta...
Neutralization (Neut) image for anti-Toll-Like Receptor 4 (TLR4) (AA 24-631) antibody (ABIN4899843) IL-8 Secretion Induced by LPS and Neutralization by Human TLR4 Antibody. Lipopolysach...
Immunocytochemistry (ICC) image for anti-Toll-Like Receptor 4 (TLR4) (AA 24-631) antibody (ABIN4899843) TLR4 in Human PBMCs. TLR4 was detected in immersion fixed human peripheral blood mono...
Immunohistochemistry (IHC) image for anti-Toll-Like Receptor 4 (TLR4) (AA 24-631) antibody (ABIN4899843) TLR4 in Human Prostate. TLR4 was detected in immersion fixed paraffin-embedded sectio...