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|Antigen||Toll-Like Receptor 5 (TLR5) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This TLR5 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-TLR5 AntibodyTarget Details TLR5 Application Details Handling Images
|Specificity||This antibody detects TLR5 at N-term.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of mouse TLR5.|
|Plasmids, Primers & others|
Target Details TLR5Product Details anti-TLR5 Antibody Application Details Handling Images back to top
|Alternative Name||TLR5 (TLR5 Antibody Abstract)|
|Background||TLR5, a Type I membrane protein belonging to the Toll-like receptor family, participates in the innate immune response to microbial agents. It also plays a role in mediating detection of bacterial flagellins. TLR5 acts via MyD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. This protein binds to TIRAP and MyD88 via their respective TIR domains TLR5 is highly expressed in liver, and is detected in lung and at very low levels in most other tissues. The TLR5 gene lies in a locus that is associated with susceptibility to Salmonella. Inbred strains of mice can be classified into 3 categories according to their resistance to infection with S.typhimurium: susceptible (BALB/c, C57BL/6, C3H/He), intermediate (DBA/2, C75L) and resistant (A, CBA). The strain MOLF/Ei is highly susceptible to the infection, has an unique TLR5 haplotype and a lower expression of TRL5.Synonyms: TIL3, Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3|
|Molecular Weight||97626 Da|
|Gene ID||7100, 10090|
|Pathways||TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-TLR5 Antibody Target Details TLR5 Handling Images back to top
ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TLR5 Antibody Target Details TLR5 Application Details Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-TLR5 Antibody Target Details TLR5 Application Details Handling back to top