anti-Mouse (Murine) TLR5 antibody for Enzyme Immunoassay

Recommended TLR5 Antibody (supplied by: Log in to see )

Antigen
Toll-Like Receptor 5 (TLR5) Antibodies
  • SLEB1
  • TIL3
  • TLR-5
  • sleb1
  • til3
  • tlr-5
  • tlr5
  • toll like receptor 5
  • toll-like receptor 5
  • toll like receptor 5 L homeolog
  • toll-like receptor 5b
  • TLR5
  • Tlr5
  • tlr5.L
  • tlr-5
  • tlr5b
Epitope
C-Term
32
15
14
11
11
11
11
10
7
7
7
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
243
146
48
43
5
2
2
2
1
Host
Rabbit
166
93
4
Clonality
Polyclonal
Conjugate
This TLR5 antibody is un-conjugated
22
17
12
9
8
5
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
207
92
83
71
63
19
16
13
11
10
6
3
3
1
Supplier
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Catalog No. ABIN357052
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN357051 EIA IHC (p) WB Rabbit Ig Fraction N-Term Log in to see Polyclonal 0
1 ABIN5555336 EIA IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
1 ABIN5555337 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 0

General

Antigen Toll-Like Receptor 5 (TLR5) Antibodies
Epitope C-Term
(32), (15), (14), (11), (11), (11), (11), (10), (7), (7), (7), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(243), (146), (48), (43), (5), (2), (2), (2), (1)
Host Rabbit
(166), (93), (4)
Clonality
Polyclonal
Conjugate This TLR5 antibody is un-conjugated
(22), (17), (12), (9), (8), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(207), (92), (83), (71), (63), (19), (16), (13), (11), (10), (6), (3), (3), (1)
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Product Details anti-TLR5 Antibody

Target Details TLR5 Application Details Handling Images
Specificity This antibody detects TLR5 at C-term.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of mouse TLR5.
Isotype Ig Fraction
Plasmids, Primers & others

Target Details TLR5

Product Details anti-TLR5 Antibody Application Details Handling Images back to top
Antigen
Alternative Name TLR5 (TLR5 Antibody Abstract)
Background TLR5, a Type I membrane protein belonging to the Toll-like receptor family, participates in the innate immune response to microbial agents. It also plays a role in mediating detection of bacterial flagellins. TLR5 acts via MyD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. This protein binds to TIRAP and MyD88 via their respective TIR domains TLR5 is highly expressed in liver, and is detected in lung and at very low levels in most other tissues. The TLR5 gene lies in a locus that is associated with susceptibility to Salmonella. Inbred strains of mice can be classified into 3 categories according to their resistance to infection with S.typhimurium: susceptible (BALB/c, C57BL/6, C3H/He), intermediate (DBA/2, C75L) and resistant (A, CBA). The strain MOLF/Ei is highly susceptible to the infection, has an unique TLR5 haplotype and a lower expression of TRL5.Synonyms: TIL3, Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3
Molecular Weight 97626 Da
Gene ID 7100, 10090
UniProt O60602
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades

Application Details

Product Details anti-TLR5 Antibody Target Details TLR5 Handling Images back to top
Application Notes ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-TLR5 Antibody Target Details TLR5 Application Details Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-TLR5 Antibody Target Details TLR5 Application Details Handling back to top
Supplier Images
 image for anti-Toll-Like Receptor 5 (TLR5) (C-Term) antibody (ABIN357052) anti-Toll-Like Receptor 5 (TLR5) (C-Term) antibody
 image for anti-Toll-Like Receptor 5 (TLR5) (C-Term) antibody (ABIN357052) anti-Toll-Like Receptor 5 (TLR5) (C-Term) antibody (Image 2)