We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Toll-Like Receptor 5 (TLR5) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This TLR5 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-TLR5 AntibodyTarget Details TLR5 Application Details Handling References for anti-TLR5 antibody (ABIN265142) Images
|Specificity||This antibody detects TLR5.|
|Immunogen||Peptide corresponding to 15 amino acids near the carboxy terminus of human TLR5|
|Plasmids, Primers & others|
Target Details TLR5Product Details anti-TLR5 Antibody Application Details Handling References for anti-TLR5 antibody (ABIN265142) Images back to top
|Alternative Name||TLR5 (TLR5 Antibody Abstract)|
|Background||Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1,2). The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors (3) so the organism can respond to potential infection. TLR5 recognizes flagellin from both Gram-positive and Gram-negative bacteria and will cause the activation of NF-κB, leading to the activation of TNF-? and other cytokines (4). A common TLR5 stop codon polymorphism that disrupts TLR5 signaling is associated with susceptibility to Legionnaires' disease and demonstrates the importance of TLR5 in the innate immune response (5). Found in the membrane, TLR5 is hghly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis (6).Synonyms: TIL3, Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3|
|Pathways||TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-TLR5 Antibody Target Details TLR5 Handling References for anti-TLR5 antibody (ABIN265142) Images back to top
Western Blot: 1-2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TLR5 Antibody Target Details TLR5 Application Details References for anti-TLR5 antibody (ABIN265142) Images back to top
|Buffer||Phosphate buffered saline with 0.02 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody (in aliquots) at -20 °C. Can be shipped at 2-8 °C.|
References for anti-TLR5 antibody (ABIN265142)Product Details anti-TLR5 Antibody Target Details TLR5 Application Details Handling Images back to top
McGettrick, ONeill: "The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction." in: Molecular immunology, Vol. 41, Issue 6-7, pp. 577-82, 2004
Takeda, Kaisho, Akira: "Toll-like receptors." in: Annual review of immunology, Vol. 21, pp. 335-76, 2003
Hawn, Verbon, Lettinga, Zhao, Li, Laws, Skerrett, Beutler, Schroeder, Nachman, Ozinsky, Smith, Aderem: "A common dominant TLR5 stop codon polymorphism abolishes flagellin signaling and is associated with susceptibility to legionnaires' disease." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1563-72, 2003
Janeway, Medzhitov: "Innate immune recognition." in: Annual review of immunology, Vol. 20, pp. 197-216, 2002
Hayashi, Smith, Ozinsky, Hawn, Yi, Goodlett, Eng, Akira, Underhill, Aderem: "The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5." in: Nature, Vol. 410, Issue 6832, pp. 1099-103, 2001
ImagesProduct Details anti-TLR5 Antibody Target Details TLR5 Application Details Handling References for anti-TLR5 antibody (ABIN265142) back to top