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|Antigen||Toll-Like Receptor 7 (TLR7) Antibodies|
|Epitope||AA 1020-1047, C-Term Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This TLR7 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|1 reference available|
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Product Details anti-TLR7 AntibodyTarget Details TLR7 Application Details Handling References for anti-TLR7 antibody (ABIN3043948) Images
|Purpose||Rabbit IgG polyclonal antibody for Toll-like receptor 7(TLR7) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.|
|Sequence||QAHPYFWQCL KNALATDNHV AYSQVFKE|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Toll-like receptor 7(TLR7) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: toll-like receptor 7
Protein Name: Toll-like receptor 7
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human TLR7 (1020-1047aa QAHPYFWQCLKNALATDNHVAYSQVFKE), different from the related mouse sequence by two amino acids.|
|Plasmids, Primers & others|
Target Details TLR7Product Details anti-TLR7 Antibody Application Details Handling References for anti-TLR7 antibody (ABIN3043948) Images back to top
|Alternative Name||TLR7 (TLR7 Antibody Abstract)|
TLR7 (Toll-like receptor 7) is protein that in humans is encoded by the TLR7 gene. Orthologs are found in mammals and birds. TLR7 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on human chromosome X. TLR7 recognises single stranded RNA in endosomes, which is a common feature of viral genomes which are internalised by macrophages.
Synonyms: PRO285 antibody|TLR 7 antibody|Tlr7 antibody|TLR7_HUMAN antibody|Toll like receptor 7 antibody|Toll-like receptor 7 antibody|UNQ248 antibody
|Pathways||TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-TLR7 Antibody Target Details TLR7 Handling References for anti-TLR7 antibody (ABIN3043948) Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for TLR7 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TLR7 Antibody Target Details TLR7 Application Details References for anti-TLR7 antibody (ABIN3043948) Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
References for anti-TLR7 antibody (ABIN3043948)Product Details anti-TLR7 Antibody Target Details TLR7 Application Details Handling Images back to top
|Product cited in:||
Esancy, Condon, Feng, Kimball, Curtright, Dhaka: "A zebrafish and mouse model for selective pruritus via direct activation of TRPA1." in: eLife, Vol. 7, 2018
ImagesProduct Details anti-TLR7 Antibody Target Details TLR7 Application Details Handling References for anti-TLR7 antibody (ABIN3043948) back to top