anti-Human TLR9 antibody for Immunoassay

Recommended TLR9 Antibody (supplied by: Log in to see )

Antigen
Toll-Like Receptor 9 (TLR9) Antibodies
  • CD289
  • toll like receptor 9
  • toll-like receptor 9
  • TLR9
  • Tlr9
Reactivity
Mouse (Murine), Human
261
147
55
36
23
20
7
5
3
2
2
2
2
2
1
1
Host
Mouse
143
123
35
14
4
3
Clonality
Monoclonal
Conjugate
This TLR9 antibody is conjugated to Biotin
26
25
17
8
6
6
5
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Application
Immunoassay (IA), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
227
162
131
109
89
39
37
37
14
7
6
6
6
5
4
3
3
3
2
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Catalog No. ABIN920704
$ 751.67
Plus shipping costs $45.00

General

Antigen Toll-Like Receptor 9 (TLR9) Antibodies
Reactivity Mouse (Murine), Human
(261), (147), (55), (36), (23), (20), (7), (5), (3), (2), (2), (2), (2), (2), (1), (1)
Host Mouse
(143), (123), (35), (14), (4), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TLR9 antibody is conjugated to Biotin
(26), (25), (17), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunoassay (IA), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
(227), (162), (131), (109), (89), (39), (37), (37), (14), (7), (6), (6), (6), (5), (4), (3), (3), (3), (2)
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Product Details anti-TLR9 Antibody

Target Details TLR9 Application Details Handling Images
Specificity Reacts with mouse TLR9. Weak cross-reactivity with human TLR9.
Sterility 0.2 μm filtered
Clone 5G5
Isotype IgG2a
Plasmids, Primers & others

Target Details TLR9

Product Details anti-TLR9 Antibody Application Details Handling Images back to top
Antigen
Alternative Name TLR9 (TLR9 Antibody Abstract)
Background Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defence of many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLR identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defence to pathogens. As investigated so far all functional characterized TLR signals via the TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems to be essential. In contrast to cell-wall components, bacterial DNA is probably invisible for immune cells until DNA is liberated during processes taking place in the endosomal/lysosomal compartment where intracellular TLR9 recruits MyD88 to initiate signal transduction. Unmethylated CpG- dinucleotide-containing sequences are found much more frequently in bacterial genomes than in vertebrates genomes, whereas the frequency of CpG dinucleotides are suppressed and usually methylated. The regions adjacent to the CpG dinucleotides also affect the immunostimulatory activity. The optimal sequence differs significantly between mammalian species. Methylated CpG dinucleotides lack immunostimulatory activities. Cellular activation in response to bacterial DNA and synthetic dinucleotides containing unmethylated CpG-dinucleotides is mediated by TLR9.
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades

Application Details

Product Details anti-TLR9 Antibody Target Details TLR9 Handling Images back to top
Application Notes Flow cytometry: Use at a 1:10 dilution. Immunoassays: (detection antibody) Immunohistochemistry: (frozen sections) stains RAW macrophages and TLR9 transfected HEK293 cells
Restrictions For Research Use only

Handling

Product Details anti-TLR9 Antibody Target Details TLR9 Application Details Images back to top
Format Liquid
Buffer 0.5 mL of 100 µg/mL 0.2 µm filtered monoclonal antibody solution in PBS containing protein stabilizer and 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C