anti-Rat (Rattus) TNFAIP3 antibody for Immunocytochemistry

Recommended TNFAIP3 Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) Antibodies
  • tnfaip3
  • MGC82339
  • TNFAIP3
  • A20
  • OTUD7C
  • TNFA1P2
  • Tnfip3
  • TNF alpha induced protein 3
  • TNF alpha induced protein 3 S homeolog
  • tumor necrosis factor, alpha-induced protein 3
  • TNFAIP3
  • tnfaip3.S
  • Tnfaip3
Epitope
C-Term
35
15
6
4
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
110
69
67
6
2
1
1
Host
Mouse
79
25
6
Clonality
Monoclonal
Conjugate
This TNFAIP3 antibody is un-conjugated
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
81
38
37
33
32
26
18
13
9
6
3
2
2
1
1
Options
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Available images

Catalog No. ABIN4277015
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13 ABIN6265632 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN1875125 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2574921 ICC IF IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 0
1 ABIN2880119 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
-1.0431099 ABIN4277012 ELISA ICC IF IHC IHC (p) WB Rabbit Center Log in to see Polyclonal 0
-4.04311 ABIN4277013 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
-10.04311 ABIN4277033 FACS ICC IF IHC (p) WB Alexa Fluor 647 Mouse IgG1 kappa C-Term Log in to see 59A426 0
-10.04311 ABIN4277032 FACS ICC IF IHC (p) WB Alexa Fluor 488 Mouse IgG1 kappa C-Term Log in to see 59A426 0
-10.04311 ABIN4995096 FACS ICC IF IHC (p) FITC Mouse IgG1 kappa C-Term Log in to see 59A426 0
-13.04311 ABIN4277014 CyTOF FACS ICC IF IHC (p) IP SimWes WB Mouse IgG1 kappa C-Term Log in to see 59A426 0
-13.04311 ABIN4277024 FACS ICC IF IHC (p) WB DyLight 755 Mouse IgG1 kappa C-Term Log in to see 59A426 0
-13.04311 ABIN4277029 FACS ICC IF IHC (p) WB DyLight 405 Mouse IgG1 kappa C-Term Log in to see 59A426 0
-13.04311 ABIN4277020 FACS ICC IF IHC (p) WB DyLight 650 Mouse IgG1 kappa C-Term Log in to see 59A426 0
-13.04311 ABIN4277025 FACS ICC IF IHC (p) IP WB PerCP Mouse IgG1 kappa C-Term Log in to see 59A426 0
-13.04311 ABIN4277022 FACS ICC IF IHC (p) WB DyLight 488 Mouse IgG1 kappa C-Term Log in to see 59A426 0
-13.04311 ABIN4277028 FACS ICC IF IHC (p) WB DyLight 350 Mouse IgG1 kappa C-Term Log in to see 59A426 0
-13.04311 ABIN4277027 FACS ICC IF IHC (p) WB DyLight 550 Mouse IgG1 kappa C-Term Log in to see 59A426 0
-13.04311 ABIN4277031 FACS ICC IF IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa C-Term Log in to see 59A426 0
-13.04311 ABIN4277034 FACS ICC IF IHC (p) WB Alexa Fluor 700 Mouse IgG1 kappa C-Term Log in to see 59A426 0
-13.04311 ABIN4277019 ICC IF IHC (p) WB Biotin Mouse IgG1 kappa C-Term Log in to see 59A426 0

General

Antigen Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) Antibodies
Epitope C-Term
(35), (15), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(110), (69), (67), (6), (2), (1), (1)
Host Mouse
(79), (25), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TNFAIP3 antibody is un-conjugated
(4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(81), (38), (37), (33), (32), (26), (18), (13), (9), (6), (3), (2), (2), (1), (1)
Pubmed 38 references available
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Product Details anti-TNFAIP3 Antibody

Target Details TNFAIP3 Application Details Handling References for anti-TNFAIP3 antibody (ABIN4277015) Images
Purification Protein G purified
Immunogen Full length recombinant human A20. The epitope has been mapped to the C-terminal portion of A20 (amino acids 440-790)
Clone 59A426
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details TNFAIP3

Product Details anti-TNFAIP3 Antibody Application Details Handling References for anti-TNFAIP3 antibody (ABIN4277015) Images back to top
Antigen
Alternative Name A20/TNFAIP3 (TNFAIP3 Antibody Abstract)
Background Gene Symbol: TNFAIP3
Gene ID 7128
UniProt P21580
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-TNFAIP3 Antibody Target Details TNFAIP3 Handling References for anti-TNFAIP3 antibody (ABIN4277015) Images back to top
Application Notes Western Blot 2-4 μg/mL, Simple Western 1:100, Flow Cytometry 1:20-1:2000, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1-2 μg/mL, Immunohistochemistry-Paraffin 1:10-1:500, CyTOF-readyThe A20 antibody (clone 59A426) has also been used in Immunohistochemistry-Paraffin (Metellus, 2010) and Flow Cytometry (intracellular) (Hjelmeland, 2010). Multiple A20 cleavage fragments have been described in Western Blot, see Coornaert (2008) and Hailfinger (2009) for additional details. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-TNFAIP3 Antibody Target Details TNFAIP3 Application Details References for anti-TNFAIP3 antibody (ABIN4277015) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-TNFAIP3 antibody (ABIN4277015)

Product Details anti-TNFAIP3 Antibody Target Details TNFAIP3 Application Details Handling Images back to top
Product cited in:

Lim, Maubach, Sokolova, Feige, Diezko, Buchbinder, Backert, Schlüter, Lavrik, Naumann: "Pathogen-induced ubiquitin-editing enzyme A20 bifunctionally shuts off NF-κB and caspase-8-dependent apoptotic cell death." in: Cell death and differentiation, Vol. 24, Issue 9, pp. 1621-1631, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Reihill, Malcomson, Bertelsen, Cheung, Czerwiec, Barsden, Elborn, Dürkop, Hirsch, Ennis, Kelly, Schock: "Induction of the inflammatory regulator A20 by gibberellic acid in airway epithelial cells." in: British journal of pharmacology, Vol. 173, Issue 4, pp. 778-89, 2016 (Sample species: Human). Further details: Western Blotting

Perez, Jukica, Listopad, Anders, Kühl, Loddenkemper, Blankenstein, Charo: "Permissive expansion and homing of adoptively transferred T cells in tumor-bearing hosts." in: International journal of cancer, Vol. 137, Issue 2, pp. 359-71, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

De, Dainichi, Rathinam, Ghosh: "The deubiquitinase activity of A20 is dispensable for NF-κB signaling." in: EMBO reports, Vol. 15, Issue 7, pp. 775-83, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Lai, Wu, Tsai, Chuang, Chuang, Hsu, Lai: "Transcription of Tnfaip3 is regulated by NF-κB and p38 via C/EBPβ in activated macrophages." in: PLoS ONE, Vol. 8, Issue 9, pp. e73153, 2013 (Sample species: Mouse (Murine)).

Svingerud, Solstad, Sun, Nyrud, Kileng, Greiner-Tollersrud, Robertsen: "Atlantic salmon type I IFN subtypes show differences in antiviral activity and cell-dependent expression: evidence for high IFNb/IFNc-producing cells in fish lymphoid tissues." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 12, pp. 5912-23, 2012

Frenzel, Claus, Plume, Schwamb, Konermann, Pallasch, Claasen, Brinker, Wollnik, Plass, Wendtner: "Sustained NF-kappaB activity in chronic lymphocytic leukemia is independent of genetic and epigenetic alterations in the TNFAIP3 (A20) locus." in: International journal of cancer, Vol. 128, Issue 10, pp. 2495-500, 2011

Imbeault, Ouellet, Giguère, Bertin, Bélanger, Martin, Tremblay: "Acquisition of host-derived CD40L by HIV-1 in vivo and its functional consequences in the B-cell compartment." in: Journal of virology, Vol. 85, Issue 5, pp. 2189-200, 2011

Adrianto, Wen, Templeton, Wiley, King, Lessard, Bates, Hu, Kelly, Kaufman, Guthridge, Alarcón-Riquelme, Anaya, Bae, Bang, Boackle, Brown, Petri, Gallant, Ramsey-Goldman, Reveille, Vila, Criswell et al.: "Association of a functional variant downstream of TNFAIP3 with systemic lupus erythematosus. ..." in: Nature genetics, Vol. 43, Issue 3, pp. 253-8, 2011

Hövelmeyer, Reissig, Xuan, Adams-Quack, Lukas, Nikolaev, Schlüter, Waisman: "A20 deficiency in B cells enhances B-cell proliferation and results in the development of autoantibodies." in: European journal of immunology, Vol. 41, Issue 3, pp. 595-601, 2011

Gerlach, Cordier, Schmukle, Emmerich, Rieser, Haas, Webb, Rickard, Anderton, Wong, Nachbur, Gangoda, Warnken, Purcell, Silke, Walczak: "Linear ubiquitination prevents inflammation and regulates immune signalling." in: Nature, Vol. 471, Issue 7340, pp. 591-6, 2011

Karube, Nakagawa, Tsuzuki, Takeuchi, Honma, Nakashima, Shimizu, Ko, Morishima, Ohshima, Nakamura, Seto: "Identification of FOXO3 and PRDM1 as tumor-suppressor gene candidates in NK-cell neoplasms by genomic and functional analyses." in: Blood, Vol. 118, Issue 12, pp. 3195-204, 2011

Hjelmeland, Wu, Wickman, Eyler, Heddleston, Shi, Lathia, Macswords, Lee, McLendon, Rich: "Targeting A20 decreases glioma stem cell survival and tumor growth." in: PLoS biology, Vol. 8, Issue 2, pp. e1000319, 2010

Harada, Kanehira, Fujisawa, Takata, Shuin, Miki, Fujioka, Nakamura, Katagiri: "Cell-permeable peptide DEPDC1-ZNF224 interferes with transcriptional repression and oncogenicity in bladder cancer cells." in: Cancer research, Vol. 70, Issue 14, pp. 5829-39, 2010

Malinverni, Unterreiner, Staal, Demeyer, Galaup, Luyten, Beyaert, Bornancin: "Cleavage by MALT1 induces cytosolic release of A20." in: Biochemical and biophysical research communications, Vol. 400, Issue 4, pp. 543-7, 2010

Li, Soetandyo, Wang, Ye: "The zinc finger protein A20 targets TRAF2 to the lysosomes for degradation." in: Biochimica et biophysica acta, Vol. 1793, Issue 2, pp. 346-53, 2009

Breckpot, Aerts-Toegaert, Heirman, Peeters, Beyaert, Aerts, Thielemans: "Attenuated expression of A20 markedly increases the efficacy of double-stranded RNA-activated dendritic cells as an anti-cancer vaccine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 2, pp. 860-70, 2009

Verhelst, Verstrepen, Coornaert, Carpentier, Beyaert: "Cellular expression of A20 and ABIN-3 in response to Toll-like receptor-4 stimulation." in: Methods in molecular biology (Clifton, N.J.), Vol. 517, pp. 205-15, 2009

Schmitz, Hansmann, Bohle, Martin-Subero, Hartmann, Mechtersheimer, Klapper, Vater, Giefing, Gesk, Stanelle, Siebert, Küppers: "TNFAIP3 (A20) is a tumor suppressor gene in Hodgkin lymphoma and primary mediastinal B cell lymphoma." in: The Journal of experimental medicine, Vol. 206, Issue 5, pp. 981-9, 2009

Düwel, Welteke, Oeckinghaus, Baens, Kloo, Ferch, Darnay, Ruland, Marynen, Krappmann: "A20 negatively regulates T cell receptor signaling to NF-kappaB by cleaving Malt1 ubiquitin chains." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 12, pp. 7718-28, 2009

Images

Product Details anti-TNFAIP3 Antibody Target Details TNFAIP3 Application Details Handling References for anti-TNFAIP3 antibody (ABIN4277015) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) (C-Term) antibody (ABIN4277015) Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Intracellular flow cytom...
Simple Western (SimWes) image for anti-Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) (C-Term) antibody (ABIN4277015) Simple Western: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Simple Western lane view...
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) (C-Term) antibody (ABIN4277015) Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Analysis of PE conjugate...
Western Blotting (WB) image for anti-Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) (C-Term) antibody (ABIN4277015) Western Blot: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Analysis of A20 using mAb ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) (C-Term) antibody (ABIN4277015) Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Formalin-...
Western Blotting (WB) image for anti-Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) (C-Term) antibody (ABIN4277015) Western Blot: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Human Jurkat lysate probed...
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) (C-Term) antibody (ABIN4277015) Flow Cytometry: A20/TNFAIP3 Antibody (59A426) - An intracellular stain was performed...
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) (C-Term) antibody (ABIN4277015) Flow Cytometry: A20/TNFAIP3 Antibody (59A426) - An intracellular stain was performed...