anti-Rat (Rattus) Hephaestin antibody for Western Blotting

Recommended Hephaestin Antibody (supplied by: Log in to see )

Antigen
Hephaestin (HEPH) Antibodies
  • HEPH
  • C130006F04Rik
  • Cpl
  • mKIAA0698
  • sla
  • CPL
  • hephaestin
  • HEPH
  • heph
  • Heph
Epitope
N-Term
6
4
3
2
2
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio)
37
26
9
1
Host
Rabbit
33
6
Clonality
Polyclonal
Conjugate
This Hephaestin antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
21
17
13
13
4
2
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN2782835
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0788064 ABIN635918 WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN321208 WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN207364 ELISA WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN1712603 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal 0
1 ABIN1715088 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN1701586 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN207383 ELISA WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN803302 ELISA WB Rabbit Log in to see Polyclonal 0

General

Antigen Hephaestin (HEPH) Antibodies
Epitope N-Term
(6), (4), (3), (2), (2), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio)
(37), (26), (9), (1)
Host Rabbit
(33), (6)
Clonality
Polyclonal
Conjugate This Hephaestin antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(21), (17), (13), (13), (4), (2), (2), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-Hephaestin Antibody

Target Details Hephaestin Application Details Handling References for anti-Hephaestin antibody (ABIN2782835) Images
Blocking Peptide Blocking peptide for this product available: ABIN977932
Sequence MHAINGFVFG NLPELNMCAQ KRVAWHLFGM GNEIDVHTAF FHGQMLTTRG
Predicted Reactivity Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%, Sheep: 93%, Zebrafish: 79%
Characteristics This is a rabbit polyclonal antibody against HEPH. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human HEPH
Plasmids, Primers & others

Target Details Hephaestin

Product Details anti-Hephaestin Antibody Application Details Handling References for anti-Hephaestin antibody (ABIN2782835) Images back to top
Antigen
Alternative Name HEPH (HEPH Antibody Abstract)
Background B1AJX8(HEPH) is similar to an iron transport protein found in mouse. The mouse protein is similar to ceruloplasmin, a serum multi-copper ferroxidase, and is thought to be a membrane-bound protein responsible for transport of dietary iron from epithelial cells of the intestinal lumen into the circulatory system. In mouse, defects in this gene can lead to severe microcytic anemia. Three transcript variants encoding different isoforms have been described for this gene. The protein encoded by this gene is similar to an iron transport protein found in mouse. The mouse protein is similar to ceruloplasmin, a serum multi-copper ferroxidase, and is thought to be a membrane-bound protein responsible for transport of dietary iron from epithelial cells of the intestinal lumen into the circulatory system. In mouse, defects in this gene can lead to severe microcytic anemia. Two transcript variants encoding different isoforms have been described for this gene.
Alias Symbols: CPL, KIAA0698
Protein Interaction Partner: FBXO6,
Protein Size: 891
Molecular Weight 100 kDa
Gene ID 9843
NCBI Accession NM_014799, NP_055614
UniProt B1AJX8
Pathways Transition Metal Ion Homeostasis

Application Details

Product Details anti-Hephaestin Antibody Target Details Hephaestin Handling References for anti-Hephaestin antibody (ABIN2782835) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 891 AA

Restrictions For Research Use only

Handling

Product Details anti-Hephaestin Antibody Target Details Hephaestin Application Details References for anti-Hephaestin antibody (ABIN2782835) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Hephaestin antibody (ABIN2782835)

Product Details anti-Hephaestin Antibody Target Details Hephaestin Application Details Handling Images back to top
Background publications

Han, Kim: "Colocalization of ferroportin-1 with hephaestin on the basolateral membrane of human intestinal absorptive cells." in: Journal of cellular biochemistry, Vol. 101, Issue 4, pp. 1000-10, 2007

Images

Product Details anti-Hephaestin Antibody Target Details Hephaestin Application Details Handling References for anti-Hephaestin antibody (ABIN2782835) back to top
Supplier Images
Western Blotting (WB) image for anti-Hephaestin (HEPH) (N-Term) antibody (ABIN2782835) WB Suggested Anti-HEPH Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Posi...