We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Prion Protein 2 (Dublet) (PRND) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product detailsTarget Details Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Doppel detection. Tested with WB, IHC-P in Human,Mouse,Rat.|
|Sequence||ATQAANQGEF QKPDNKLHQQ VLWRLVQELC SLKH|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
|Characteristics||Rabbit IgG polyclonal antibody for Doppel detection. Tested with WB, IHC-P in Human,Mouse,Rat.|
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence of human Doppel (ATQAANQGEFQKPDNKLHQQVLWRLVQELCSLKH).|
Target DetailsProduct details Application Details Handling Images back to top
|Alternative Name||PRND (PRND Antibody Abstract)|
Synonyms: Prion-like protein doppel, PrPLP, Prion protein 2, PRND, DPL, UNQ1830/PRO3443
Background: Prion protein 2 (dublet), also known as PRND, or Doppel protein, is a protein which in humans is encoded by the PRND gene. It is mapped to 20p13. This gene is found on chromosome 20, approximately 20 kbp downstream of the gene encoding cellular prion protein, to which it is biochemically and structurally similar. The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that is found predominantly in testis. Mutations in this gene may lead to neurological disorders.
|Pathways||Transition Metal Ion Homeostasis|
Application DetailsProduct details Target Details Handling Images back to top
Application details: Western blot|0.1-0.5&mu,g/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1&mu,g/mL
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
|Restrictions||For Research Use only|
HandlingProduct details Target Details Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C,-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.