anti-Human S100A9 antibody for Immunohistochemistry

Recommended S100A9 Antibody (supplied by: Log in to see )

Antigen
S100 Calcium Binding Protein A9 (S100A9) Antibodies
  • 60B8AG
  • CAGB
  • CFAG
  • CGLB
  • L1AG
  • LIAG
  • MAC387
  • MIF
  • MRP14
  • NIF
  • P14
  • Mrp14
  • S100A9
  • MRP-14
  • p14
  • 60B8Ag
  • AW546964
  • BEE22
  • Cagb
  • GAGB
  • L1Ag
  • S100 calcium binding protein A9
  • S100 calcium binding protein A9 (calgranulin B)
  • S100A9
  • S100a9
Epitope
AA 67-79
30
18
15
13
10
7
7
4
3
2
2
2
1
1
1
1
Reactivity
Human
374
104
55
8
3
1
1
1
1
Host
Goat
263
120
21
3
2
2
Clonality
Polyclonal
Conjugate
This S100A9 antibody is un-conjugated
36
21
15
13
10
7
6
5
5
5
5
5
5
5
5
5
5
5
5
5
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
185
158
151
105
101
101
99
27
16
15
13
12
9
5
3
2
1
1
1
Supplier
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Catalog No. ABIN462299
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.858188 ABIN959546 ELISA IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see 47-8D3 0
8.858188 ABIN957822 IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
8.858188 ABIN2466632 IHC ELISA WB Goat Internal Region Log in to see Polyclonal 0
8.858188 ABIN5963190 IHC WB Rabbit IgG Log in to see Polyclonal 0
8.858188 ABIN4351787 IHC WB Mouse IgG2a Log in to see 10D5 0
8.858188 ABIN5862256 ELISA IHC Goat Log in to see Polyclonal 0
8.858188 ABIN2433719 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
1 ABIN2786758 IHC WB Rabbit N-Term Log in to see Polyclonal 1
1 ABIN409938 ICC IF IHC IHC (p) SimWes WB Rabbit Log in to see Polyclonal 6
1 ABIN449656 IHC (p) IHC ELISA WB Goat Log in to see Polyclonal 2
1 ABIN4351767 IHC IHC (p) SimWes WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5945966 ICC IF IHC IHC (p) WB Rabbit IgG AA 1-100 Log in to see JF096-8 0
1 ABIN379944 ELISA IHC IHC (p) Mouse Log in to see S36-48 0
1 ABIN1175036 IHC ELISA WB Biotin Rabbit IgG AA 1-114 Log in to see Polyclonal 0
1 ABIN1078513 ICC IHC WB Rabbit IgG AA 1-114 Log in to see Polyclonal 0
1 ABIN5680378 FACS ICC IHC WB Sheep IgG Log in to see Polyclonal 0
1 ABIN6264908 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2579958 IHC IHC (p) WB Mouse IgG1 Log in to see 2C5 0
1 ABIN2579963 IHC IHC (p) WB Mouse IgG1 Log in to see 11A12 0
1 ABIN2579962 IHC IHC (p) Mouse IgG1 Log in to see 3C12 0

General

Antigen S100 Calcium Binding Protein A9 (S100A9) Antibodies
Epitope AA 67-79
(30), (18), (15), (13), (10), (7), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(374), (104), (55), (8), (3), (1), (1), (1), (1)
Host Goat
(263), (120), (21), (3), (2), (2)
Clonality
Polyclonal
Conjugate This S100A9 antibody is un-conjugated
(36), (21), (15), (13), (10), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(185), (158), (151), (105), (101), (101), (99), (27), (16), (15), (13), (12), (9), (5), (3), (2), (1), (1), (1)
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Product Details anti-S100A9 Antibody

Target Details S100A9 Application Details Handling Images
Brand IHC-plus™
Specificity Human S100A9.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Hamster, Bovine, Rabbit, Horse, Pig (92%) Panda (85%).
Purification Immunoaffinity purified
Immunogen Synthetic peptide C-DTNADKQLSFEEF from an internal region of human S100A9 (NP_002956.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Hamster, Bovine, Rabbit, Horse, Pig (92%), Panda (85%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details S100A9

Product Details anti-S100A9 Antibody Application Details Handling Images back to top
Antigen
Alternative Name S100A9 / MRP14 (S100A9 Antibody Abstract)
Background Name/Gene ID: S100A9

Synonyms: S100A9, 60B8AG, Calgranulin B, CFAG, CAGB, Calgranulin-B, Calprotectin L1H subunit, CGLB, L1Ag, MRP14, NIF, p14, Protein S100-A9, LIAG, MAC387, MRP-14
Gene ID 6280
NCBI Accession NP_002956
UniProt P06702
Pathways Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-S100A9 Antibody Target Details S100A9 Handling Images back to top
Application Notes Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:8000, 0.3 - 1 μg/mL), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 16kD band observed in Human Peripheral Mononucleocytes lysates (calculated MW of 13.2kD according to NP_002956.1). The observed molecular weight corresponds to earlier findings (~15kD) in literature with different antibodies (McCormick et al, Biol Chem. 2005 Dec 16,280(50):41521-9., PMID: 16216873). Recommended concentration: 0.3-1 μg/mL.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-S100A9 Antibody Target Details S100A9 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice avoid freeze thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Images

Product Details anti-S100A9 Antibody Target Details S100A9 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-S100 Calcium Binding Protein A9 (S100A9) (AA 67-79) antibody (ABIN462299) Human Liver (formalin-fixed, paraffin-embedded) stained with S100A9 antibody ABIN4622...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-S100 Calcium Binding Protein A9 (S100A9) (AA 67-79) antibody (ABIN462299) Human Lung (formalin-fixed, paraffin-embedded) stained with S100A9 antibody ABIN46229...
Immunohistochemistry (IHC) image for anti-S100 Calcium Binding Protein A9 (S100A9) (AA 67-79) antibody (ABIN462299) Anti-S100A9 / MRP14 antibody IHC of human liver. Immunohistochemistry of formalin-fix...