anti-Rat (Rattus) S100A9 antibody for Western Blotting

Recommended S100A9 Antibody (supplied by: Log in to see )

Antigen
S100 Calcium Binding Protein A9 (S100A9) Antibodies
  • 60B8AG
  • CAGB
  • CFAG
  • CGLB
  • L1AG
  • LIAG
  • MAC387
  • MIF
  • MRP14
  • NIF
  • P14
  • Mrp14
  • S100A9
  • MRP-14
  • p14
  • 60B8Ag
  • AW546964
  • BEE22
  • Cagb
  • GAGB
  • L1Ag
  • S100 calcium binding protein A9
  • S100 calcium binding protein A9 (calgranulin B)
  • S100A9
  • S100a9
Epitope
AA 2-113
30
18
15
13
10
8
6
3
2
2
2
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
374
103
54
8
3
1
1
1
1
Host
Rabbit
263
120
22
3
2
2
Clonality
Polyclonal
Conjugate
This S100A9 antibody is un-conjugated
36
21
15
13
10
7
6
5
5
5
5
5
5
5
5
5
5
5
5
5
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
185
159
151
105
103
101
99
27
16
15
13
12
9
5
3
2
1
1
1
Options
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Catalog No. ABIN3042482
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10.792003 ABIN409938 ICC IF IHC IHC (p) SimWes WB Rabbit Log in to see Polyclonal 6
10.789729 ABIN6572042 IHC WB Rabbit IgG Log in to see Polyclonal 0
7.7897296 ABIN6264908 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
7.7897296 ABIN1078515 ICC IHC WB Rabbit IgG AA 1-113 Log in to see Polyclonal 0
7.7897296 ABIN2910263 IF/ICC IHC IP WB Rabbit AA 1-113 Log in to see Polyclonal 0
4.7897296 ABIN4905081 WB Rabbit IgG Log in to see Polyclonal 0
4.7897296 ABIN3068747 WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5514666 WB Rabbit Middle Region Log in to see Polyclonal 0
1 ABIN2627821 WB Rabbit IgG AA 1-113 Log in to see Polyclonal 0
1 ABIN2627827 WB FITC Rabbit IgG AA 1-113 Log in to see Polyclonal 0
1 ABIN2627825 WB Biotin Rabbit IgG AA 1-113 Log in to see Polyclonal 0
1 ABIN5661352 WB Rabbit Log in to see Polyclonal 0
1 ABIN3023909 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see 47-8D3 0
1 ABIN6296494 FACS IF IHC WB Mouse Log in to see 0
1 ABIN1175038 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN3023910 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see 47-8D3 0
1 ABIN4952454 ELISA IHC (p) WB Rabbit IgG AA 2-113 Log in to see Polyclonal 0
1 ABIN6053549 IF/ICC IHC WB Biotin Rabbit IgG Log in to see Polyclonal 0

General

Antigen S100 Calcium Binding Protein A9 (S100A9) Antibodies
Epitope AA 2-113
(30), (18), (15), (13), (10), (8), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(374), (103), (54), (8), (3), (1), (1), (1), (1)
Host Rabbit
(263), (120), (22), (3), (2), (2)
Clonality
Polyclonal
Conjugate This S100A9 antibody is un-conjugated
(36), (21), (15), (13), (10), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(185), (159), (151), (105), (103), (101), (99), (27), (16), (15), (13), (12), (9), (5), (3), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-S100A9 Antibody

Target Details S100A9 Application Details Handling References for anti-S100A9 antibody (ABIN3042482) Images
Purpose Rabbit IgG polyclonal antibody for Protein S100-A9(S100A9) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Protein S100-A9(S100A9) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.
Gene Name: S100 calcium binding protein A9
Protein Name: Protein S100-A9
Purification Immunogen affinity purified.
Immunogen E. coli-derived mouse S100A9 recombinant protein (Position: A2-K113). Mouse S100A9 shares 59.8% and 78.6% amino acid (aa) sequence identity with human and rat S100A9, respectively.
Isotype IgG
Plasmids, Primers & others

Target Details S100A9

Product Details anti-S100A9 Antibody Application Details Handling References for anti-S100A9 antibody (ABIN3042482) Images back to top
Antigen
Alternative Name S100A9 (S100A9 Antibody Abstract)
Background S100 calcium-binding protein A9 (S100A9), also known as migration inhibitory factor-related protein 14 (MRP14) or calgranulin B, is a protein that in humans is encoded by the S100A9 gene. S100-A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. And S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase.

Synonyms: Leukocyte L1 complex heavy chain antibody|60B8AG antibody|AW546964 antibody|BEE22 antibody|CAGB antibody|Calgranulin B antibody| Calgranulin-B antibody|Calprotectin L1H subunit antibody|CFAG antibody|CGLB antibody|Cystic fibrosis antigen antibody|Cystic fibrosis antigen B antibody|HGNC:10499 antibody|L1AG antibody|Leukocyte L1 complex heavy chain antibody|LIAG antibody|MAC 387 antibody|MAC387 antibody|MIF antibody|Migration inhibitory factor related protein 14 antibody|Migration inhibitory factor-related protein 14 antibody| MRP 14 antibody|MRP 8 antibody|MRP-14 antibody|MRP14 antibody|Myeloid-related protein 14 antibody|NIF antibody| OTTHUMP00000015331 antibody|P14 antibody|Protein S100-A9 antibody|S100 A9 antibody|S100 calcium binding protein A9 antibody|S100 calcium binding protein A9 calgranulin B antibody|S100 calcium-binding protein A9 antibody|S100A9 antibody| S10A9_HUMAN antibody
Gene ID 20202
UniProt P31725
Pathways Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-S100A9 Antibody Target Details S100A9 Handling References for anti-S100A9 antibody (ABIN3042482) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-S100A9 Antibody Target Details S100A9 Application Details References for anti-S100A9 antibody (ABIN3042482) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-S100A9 antibody (ABIN3042482)

Product Details anti-S100A9 Antibody Target Details S100A9 Application Details Handling Images back to top
Product cited in:

Liu, Liu, He, Zhang, Liu, Wang, Qiu, Xu, Xiang, Lv: "Differential gene and lncRNA expression in the lower thoracic spinal cord following ischemia/reperfusion-induced acute kidney injury in rats." in: Oncotarget, Vol. 8, Issue 32, pp. 53465-53481, 2017

Yan, Tian, Wang, Cheng, Xu, Song, Zhang, Zhang: "Age dependent changes in cartilage matrix, subchondral bone mass, and estradiol levels in blood serum, in naturally occurring osteoarthritis in Guinea pigs." in: International journal of molecular sciences, Vol. 15, Issue 8, pp. 13578-95, 2015

Images

Product Details anti-S100A9 Antibody Target Details S100A9 Application Details Handling References for anti-S100A9 antibody (ABIN3042482) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-S100 Calcium Binding Protein A9 (S100A9) (AA 2-113) antibody (ABIN3042482) Anti- S100A9 Picoband antibody, IHC(P) IHC(P): Rat Spleen Tissue
Immunohistochemistry (IHC) image for anti-S100 Calcium Binding Protein A9 (S100A9) (AA 2-113) antibody (ABIN3042482) Anti- S100A9 Picoband antibody, IHC(P) IHC(P): Mouse Spleen Tissue
Western Blotting (WB) image for anti-S100 Calcium Binding Protein A9 (S100A9) (AA 2-113) antibody (ABIN3042482) Observed bind size: 13KD