anti-Rat (Rattus) S100A9 antibody for Western Blotting

Recommended S100A9 Antibody (supplied by: Log in to see )

Antigen
S100 Calcium Binding Protein A9 (S100A9) Antibodies
  • 60B8AG
  • CAGB
  • CFAG
  • CGLB
  • L1AG
  • LIAG
  • MAC387
  • MIF
  • MRP14
  • NIF
  • P14
  • Mrp14
  • S100A9
  • MRP-14
  • p14
  • 60B8Ag
  • AW546964
  • BEE22
  • Cagb
  • GAGB
  • L1Ag
  • S100 calcium binding protein A9
  • S100 calcium binding protein A9 (calgranulin B)
  • S100A9
  • S100a9
Reactivity
Human, Mouse (Murine), Rat (Rattus)
364
101
53
7
3
1
1
1
1
Host
Rabbit
254
117
22
3
2
2
Clonality
Polyclonal
Conjugate
This S100A9 antibody is un-conjugated
33
21
15
13
10
7
6
5
5
5
5
5
5
5
5
5
5
5
5
5
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
181
155
143
105
103
96
94
27
17
14
13
11
8
5
3
1
1
1
1
1
Supplier
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Catalog No. ABIN409938
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.8724732 ABIN3042482 IHC (p) ELISA WB Rabbit IgG AA 2-113 Log in to see Polyclonal 0
2.8724732 ABIN1078515 ICC IHC WB Rabbit IgG AA 1-113 Log in to see Polyclonal 0
2.8724732 ABIN4905081 WB Rabbit IgG Log in to see Polyclonal 0
2.8724732 ABIN5963190 IHC WB Rabbit IgG Log in to see Polyclonal 0
2.8724732 ABIN3068747 WB Rabbit IgG Log in to see Polyclonal 0
2.8724732 ABIN2910263 IF/ICC IHC IP WB Rabbit AA 1-113 Log in to see Polyclonal 0
1 ABIN5514666 WB Rabbit Middle Region Log in to see Polyclonal 0
1 ABIN2627821 WB Rabbit IgG AA 1-113 Log in to see Polyclonal 0
1 ABIN2627825 WB Biotin Rabbit IgG AA 1-113 Log in to see Polyclonal 0
1 ABIN2627827 WB FITC Rabbit IgG AA 1-113 Log in to see Polyclonal 0
1 ABIN5661352 WB Rabbit Log in to see Polyclonal 0
1 ABIN3023909 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see 47-8D3 0
1 ABIN1175038 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN3023910 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see 47-8D3 0
1 ABIN4952454 ELISA IHC (p) WB Rabbit IgG AA 2-113 Log in to see Polyclonal 0
1 ABIN6053549 IF/ICC IHC WB Biotin Rabbit IgG Log in to see Polyclonal 0

General

Antigen S100 Calcium Binding Protein A9 (S100A9) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(364), (101), (53), (7), (3), (1), (1), (1), (1)
Host Rabbit
(254), (117), (22), (3), (2), (2)
Clonality
Polyclonal
Conjugate This S100A9 antibody is un-conjugated
(33), (21), (15), (13), (10), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(181), (155), (143), (105), (103), (96), (94), (27), (17), (14), (13), (11), (8), (5), (3), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-S100A9 Antibody

Target Details S100A9 Application Details Handling References for anti-S100A9 antibody (ABIN409938) Images
Purification Unpurified
Immunogen Full length human S100A9 protein [Swiss-Prot# P06702] expressed in E. coli.
Plasmids, Primers & others

Target Details S100A9

Product Details anti-S100A9 Antibody Application Details Handling References for anti-S100A9 antibody (ABIN409938) Images back to top
Antigen
Alternative Name S100A9 (S100A9 Antibody Abstract)
Background Gene Symbol: S100A9
Molecular Weight Theoretical MW: 16 kDa
Gene ID 6280
UniProt P06702
Pathways Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-S100A9 Antibody Target Details S100A9 Handling References for anti-S100A9 antibody (ABIN409938) Images back to top
Application Notes Western Blot 1:1000, Simple Western 1:100, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry-ParaffinThis S100A9 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at approx. 16 kDa.Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID: 21653680) Use in Immunohistochemistry reported in scientific literature (PMID 25792748) In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for S100A9 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MES) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-S100A9 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-S100A9 Antibody Target Details S100A9 Application Details References for anti-S100A9 antibody (ABIN409938) Images back to top
Format Liquid
Buffer Whole antisera
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-S100A9 antibody (ABIN409938)

Product Details anti-S100A9 Antibody Target Details S100A9 Application Details Handling Images back to top
Product cited in:

Ikubo, Yamanishi, Doe, Hashimoto, Sumida, Watanabe, El-Darawish, Li, Okamura, Yamanishi, Matsunaga: "Molecular analysis of the mouse brain exposed to chronic mild stress: The influence of hepatocyte nuclear factor 4α on physiological homeostasis." in: Molecular medicine reports, Vol. 16, Issue 1, pp. 301-309, 2017

Chernov, Dolkas, Hoang, Angert, Srikrishna, Vogl, Baranovskaya, Strongin, Shubayev: "The calcium-binding proteins S100A8 and S100A9 initiate the early inflammatory program in injured peripheral nerves." in: The Journal of biological chemistry, Vol. 290, Issue 18, pp. 11771-84, 2015 (Sample species: Rat (Rattus)). Further details: Western Blotting,Immunohistochemistry

Zhang, Ai, Li, She, Li, Tang, Qin, Ye, Tian, Li, Shen, Ma: "Inflammation-induced S100A8 activates Id3 and promotes colorectal tumorigenesis." in: International journal of cancer, Vol. 137, Issue 12, pp. 2803-14, 2015 Further details: Immunohistochemistry (Paraffin-embedded Sections)

Gaddy, Radin, Loh, Piazuelo, Kehl-Fie, Delgado, Ilca, Peek, Cover, Chazin, Skaar, Scott Algood: "The host protein calprotectin modulates the Helicobacter pylori cag type IV secretion system via zinc sequestration." in: PLoS pathogens, Vol. 10, Issue 10, pp. e1004450, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Sumi, Shimizu, Himeno: "Involvement of Nrf2 activation in the upregulation of S100A9 by exposure to inorganic arsenite." in: International journal of molecular medicine, Vol. 31, Issue 1, pp. 259-64, 2012

Pitteri, Kelly-Spratt, Gurley, Kennedy, Buson, Chin, Wang, Zhang, Wong, Chodosh, Nelson, Hanash, Kemp: "Tumor microenvironment-derived proteins dominate the plasma proteome response during breast cancer induction and progression." in: Cancer research, Vol. 71, Issue 15, pp. 5090-100, 2011 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Images

Product Details anti-S100A9 Antibody Target Details S100A9 Application Details Handling References for anti-S100A9 antibody (ABIN409938) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-S100 Calcium Binding Protein A9 (S100A9) antibody (ABIN409938) anti-S100 Calcium Binding Protein A9 (S100A9) antibody
Western Blotting (WB) image for anti-S100 Calcium Binding Protein A9 (S100A9) antibody (ABIN409938) Western Blot: S100A9 Antibody [ABIN4099386] - Cells were transfected with the pCMV6-E...
Western Blotting (WB) image for anti-S100 Calcium Binding Protein A9 (S100A9) antibody (ABIN409938) Western Blot: S100A9 Antibody [ABIN4099386] - Analysis of S100A9 Antibody in DMSO tre...
Simple Western (SimWes) image for anti-S100 Calcium Binding Protein A9 (S100A9) antibody (ABIN409938) Simple Western: S100A9 Antibody [ABIN4099386] - Simple Western lane view shows a spec...