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|Antigen||Nucleoporin 50kDa (NUP50) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This NUP50 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-NUP50 AntibodyTarget Details NUP50 Application Details Handling References for anti-NUP50 antibody (ABIN357219) Images
|Specificity||This antibody detects NUP50 at C-term.|
|Purification||Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human NUP50.|
|Plasmids, Primers & others|
Target Details NUP50Product Details anti-NUP50 Antibody Application Details Handling References for anti-NUP50 antibody (ABIN357219) Images back to top
|Alternative Name||NUP50 (NUP50 Antibody Abstract)|
|Background||The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. NUP50 may serve as a binding site on the nuclear side of the pore complex for export receptor-cargo complexes. It interacts with multiple transport receptor proteins including p27Kip1. This interaction is required for correct intracellular transport and degradation of p27Kip1.Synonyms: NPAP60L, Nuclear pore-associated protein 60 kDa-like, Nucleoporin 50 kDa|
|Molecular Weight||46863 Da|
Application DetailsProduct Details anti-NUP50 Antibody Target Details NUP50 Handling References for anti-NUP50 antibody (ABIN357219) Images back to top
ELISA 1: 1,000. Western blot 1: 100 - 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-NUP50 Antibody Target Details NUP50 Application Details References for anti-NUP50 antibody (ABIN357219) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.|
References for anti-NUP50 antibody (ABIN357219)Product Details anti-NUP50 Antibody Target Details NUP50 Application Details Handling Images back to top
Swaminathan, Melchior: "Nucleocytoplasmic transport: more than the usual suspects." in: Developmental cell, Vol. 3, Issue 3, pp. 304-6, 2002
Lindsay, Plafker, Smith, Clurman, Macara: "Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import." in: Cell, Vol. 110, Issue 3, pp. 349-60, 2002
Smitherman, Lee, Swanger, Kapur, Clurman: "Characterization and targeted disruption of murine Nup50, a p27(Kip1)-interacting component of the nuclear pore complex." in: Molecular and cellular biology, Vol. 20, Issue 15, pp. 5631-42, 2000
Guan, Kehlenbach, Schirmer, Kehlenbach, Fan, Clurman, Arnheim, Gerace: "Nup50, a nucleoplasmically oriented nucleoporin with a role in nuclear protein export." in: Molecular and cellular biology, Vol. 20, Issue 15, pp. 5619-30, 2000
ImagesProduct Details anti-NUP50 Antibody Target Details NUP50 Application Details Handling References for anti-NUP50 antibody (ABIN357219) back to top