anti-Human NUP50 antibody for ELISA

Recommended NUP50 Antibody

Antigen
Nucleoporin 50kDa (NUP50) Antibodies
  • wu:fa56e03
  • wu:fb78a08
  • GB18194
  • npap60
  • npap60l
  • Nup50
  • Rtp60
  • 1700030K07Rik
  • AI413123
  • Npap60
  • NPAP60
  • NPAP60L
  • nucleoporin 50
  • nuclear pore complex protein Nup50
  • nucleoporin 50kDa S homeolog
  • nucleoporin 50kDa
  • nup50
  • LOC410864
  • NUP50
  • nup50.S
  • CpipJ_CPIJ012737
  • Nup50
Epitope
C-Term
20
10
8
8
7
4
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Human
111
40
29
9
3
3
3
2
2
1
1
Host
Goat
80
18
18
Clonality
Polyclonal
Conjugate
This NUP50 antibody is un-conjugated
6
5
5
4
4
4
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
90
54
19
13
13
10
5
4
4
2
Options

Available images

Catalog No. ABIN184968
$ 429.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
13.943781 ABIN948988 ELISA WB Mouse IgG2b kappa AA 342-439 4H7 0
13.551319 ABIN2147911 IF ELISA WB Rabbit IgG Polyclonal 0
11.5 ABIN544667 ELISA WB Rabbit Polyclonal 3
11.5 ABIN547050 ELISA WB Goat Polyclonal 1
10 ABIN3032078 ELISA WB Rabbit Ig Fraction AA 432-461 Polyclonal 0
10 ABIN2430575 IHC ELISA WB Rabbit IgG Polyclonal 0
7 ABIN2466027 ELISA WB Goat C-Term Polyclonal 0
7 ABIN5872779 ELISA WB Goat Polyclonal 0
7 ABIN2423911 IHC ELISA WB Rabbit IgG Polyclonal 0
4 ABIN962675 ELISA IHC IHC (p) WB Goat AA 429-440 Polyclonal 0
4 ABIN2463505 ELISA WB Rabbit Polyclonal 0
4 ABIN1737602 IHC (p) ELISA WB Goat C-Term Polyclonal 0
4 ABIN2564195 IHC ELISA WB Goat IgG C-Term Polyclonal 0
4 ABIN4951960 ELISA IHC WB Goat Ig Fraction Polyclonal 0
1 ABIN2316597 ELISA WB PE Goat IgG C-Term Polyclonal 0
1 ABIN2316614 ELISA WB Alkaline Phosphatase (AP) Goat IgG C-Term Polyclonal 0
1 ABIN2316585 ELISA WB APC Goat IgG C-Term Polyclonal 0
1 ABIN2316588 ELISA WB Biotin Goat IgG C-Term Polyclonal 0
1 ABIN2316591 ELISA WB FITC Goat IgG C-Term Polyclonal 0
1 ABIN2316617 ELISA WB Rabbit IgG AA 223-252, Center, Internal Region Polyclonal 0

General

Antigen Nucleoporin 50kDa (NUP50) Antibodies
Epitope C-Term
(20), (10), (8), (8), (7), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(111), (40), (29), (9), (3), (3), (3), (2), (2), (1), (1)
Host Goat
(80), (18), (18)
Clonality
Polyclonal
Conjugate This NUP50 antibody is un-conjugated
(6), (5), (5), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(90), (54), (19), (13), (13), (10), (5), (4), (4), (2)
Pubmed 2 references available
Supplier

Product Details anti-NUP50 Antibody

Target Details NUP50 Application Details Handling References for anti-NUP50 antibody (ABIN184968) Images
Purpose Npap60 / Nup50
Blocking Peptide Blocking peptide for this product available: ABIN369018
Sequence ELHKILLEKK DA
Specificity This antibody is expected to recognise both reported isoforms (NP_009103.2, NP_705931.1).
Predicted Reactivity Human, Mouse, Rat, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-ELHKILLEKKDA, from the C Terminus of the protein sequence according to NP_009103.2, NP_705931.1.
Plasmids, Primers & others

Target Details NUP50

Product Details anti-NUP50 Antibody Application Details Handling References for anti-NUP50 antibody (ABIN184968) Images back to top
Antigen
Alternative Name NUP50 (NUP50 Antibody Abstract)
Background NUP50, NPAP60, NPAP60L, MGC39961, DKFZP564A043, nucleoporin 50 kDa, nuclear pore-associated protein 60L, CTA-268H5.7, OTTHUMP00000199530
Gene ID 10762
NCBI Accession NP_009103, NP_705931
Pathways Tube Formation

Application Details

Product Details anti-NUP50 Antibody Target Details NUP50 Handling References for anti-NUP50 antibody (ABIN184968) Images back to top
Application Notes

DS WB Results: Approx 60 kDa band observed in lysates of cell line Jurkat (calculated MW 50.1 kDa according to NP_009103.2). Recommended for use at 0.2-1 μg/mL.

DS IHC Results: In paraffin embedded Human Uterus shows textured nuclear staining of endometrial cells. Recommended concentration, 2.5-3.8 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions For Research Use only

Handling

Product Details anti-NUP50 Antibody Target Details NUP50 Application Details References for anti-NUP50 antibody (ABIN184968) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-NUP50 antibody (ABIN184968)

Product Details anti-NUP50 Antibody Target Details NUP50 Application Details Handling Images back to top
Product cited in:

Shitashige, Satow, Honda, Ono, Hirohashi, Yamada: "Regulation of Wnt signaling by the nuclear pore complex." in: Gastroenterology, Vol. 134, Issue 7, pp. 1961-71, 1971.e1-4, 2008

Background publications

Lindsay, Plafker, Smith, Clurman, Macara: "Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import." in: Cell, Vol. 110, Issue 3, pp. 349-60, 2002

Images

Product Details anti-NUP50 Antibody Target Details NUP50 Application Details Handling References for anti-NUP50 antibody (ABIN184968) back to top
Supplier Images
Western Blotting (WB) image for anti-Nucleoporin 50kDa (NUP50) (C-Term) antibody (ABIN184968) ABIN184968 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per ...