anti-Mouse (Murine) NUP50 antibody for Enzyme Immunoassay

Recommended NUP50 Antibody

Antigen
Nucleoporin 50kDa (NUP50) Antibodies
  • wu:fa56e03
  • wu:fb78a08
  • GB18194
  • npap60
  • npap60l
  • Nup50
  • Rtp60
  • 1700030K07Rik
  • AI413123
  • Npap60
  • NPAP60
  • NPAP60L
  • nucleoporin 50
  • nuclear pore complex protein Nup50
  • nucleoporin 50kDa S homeolog
  • nucleoporin 50kDa
  • nup50
  • LOC410864
  • NUP50
  • nup50.S
  • CpipJ_CPIJ012737
  • Nup50
Epitope
C-Term
20
10
8
8
7
4
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
111
39
29
9
3
3
3
2
2
1
1
Host
Rabbit
79
19
18
Clonality
Polyclonal
Conjugate
This NUP50 antibody is un-conjugated
6
5
5
4
4
4
2
2
2
2
2
2
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
90
55
20
13
13
10
5
4
4
1
Options

Available images

Catalog No. ABIN357219
$ 484.00
Plus shipping costs $45.00

General

Antigen Nucleoporin 50kDa (NUP50) Antibodies
Epitope C-Term
(20), (10), (8), (8), (7), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(111), (39), (29), (9), (3), (3), (3), (2), (2), (1), (1)
Host Rabbit
(79), (19), (18)
Clonality
Polyclonal
Conjugate This NUP50 antibody is un-conjugated
(6), (5), (5), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(90), (55), (20), (13), (13), (10), (5), (4), (4), (1)
Pubmed 4 references available
Supplier

Product Details anti-NUP50 Antibody

Target Details NUP50 Application Details Handling References for anti-NUP50 antibody (ABIN357219) Images
Specificity This antibody detects NUP50 at C-term.
Purification Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human NUP50.
Isotype Ig Fraction
Plasmids, Primers & others

Target Details NUP50

Product Details anti-NUP50 Antibody Application Details Handling References for anti-NUP50 antibody (ABIN357219) Images back to top
Antigen
Alternative Name NUP50 (NUP50 Antibody Abstract)
Background The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. NUP50 may serve as a binding site on the nuclear side of the pore complex for export receptor-cargo complexes. It interacts with multiple transport receptor proteins including p27Kip1. This interaction is required for correct intracellular transport and degradation of p27Kip1.Synonyms: NPAP60L, Nuclear pore-associated protein 60 kDa-like, Nucleoporin 50 kDa
Molecular Weight 46863 Da
Gene ID 10762
NCBI Accession NP_009103
UniProt Q9UKX7
Pathways Tube Formation

Application Details

Product Details anti-NUP50 Antibody Target Details NUP50 Handling References for anti-NUP50 antibody (ABIN357219) Images back to top
Application Notes ELISA 1: 1,000. Western blot 1: 100 - 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-NUP50 Antibody Target Details NUP50 Application Details References for anti-NUP50 antibody (ABIN357219) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-NUP50 antibody (ABIN357219)

Product Details anti-NUP50 Antibody Target Details NUP50 Application Details Handling Images back to top
Background publications

Swaminathan, Melchior: "Nucleocytoplasmic transport: more than the usual suspects." in: Developmental cell, Vol. 3, Issue 3, pp. 304-6, 2002

Lindsay, Plafker, Smith, Clurman, Macara: "Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import." in: Cell, Vol. 110, Issue 3, pp. 349-60, 2002

Smitherman, Lee, Swanger, Kapur, Clurman: "Characterization and targeted disruption of murine Nup50, a p27(Kip1)-interacting component of the nuclear pore complex." in: Molecular and cellular biology, Vol. 20, Issue 15, pp. 5631-42, 2000

Guan, Kehlenbach, Schirmer, Kehlenbach, Fan, Clurman, Arnheim, Gerace: "Nup50, a nucleoplasmically oriented nucleoporin with a role in nuclear protein export." in: Molecular and cellular biology, Vol. 20, Issue 15, pp. 5619-30, 2000

Images

Product Details anti-NUP50 Antibody Target Details NUP50 Application Details Handling References for anti-NUP50 antibody (ABIN357219) back to top
Supplier Images
 image for anti-Nucleoporin 50kDa (NUP50) (C-Term) antibody (ABIN357219) anti-Nucleoporin 50kDa (NUP50) (C-Term) antibody
 image for anti-Nucleoporin 50kDa (NUP50) (C-Term) antibody (ABIN357219) anti-Nucleoporin 50kDa (NUP50) (C-Term) antibody (Image 2)