anti-Mouse (Murine) NUP50 antibody for Western Blotting

Recommended NUP50 Antibody (supplied by: Log in to see )

Antigen
Nucleoporin 50kDa (NUP50) Antibodies
  • wu:fa56e03
  • wu:fb78a08
  • GB18194
  • npap60
  • npap60l
  • Nup50
  • Rtp60
  • 1700030K07Rik
  • AI413123
  • Npap60
  • NPAP60
  • NPAP60L
  • nucleoporin 50
  • nuclear pore complex protein Nup50
  • nucleoporin 50kDa S homeolog
  • nucleoporin 50kDa
  • nup50
  • LOC410864
  • NUP50
  • nup50.S
  • CpipJ_CPIJ012737
  • Nup50
Epitope
C-Term
21
11
9
8
7
5
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
107
40
29
9
3
3
3
2
2
1
1
Host
Rabbit
74
19
19
Clonality
Polyclonal
Conjugate
This NUP50 antibody is un-conjugated
5
5
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
93
55
20
13
12
9
5
4
3
2
Options
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Available images

Catalog No. ABIN2856374
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10.843901 ABIN1860084 ICC IHC WB Rabbit IgG AA 134-393 Log in to see Polyclonal 0
10.843901 ABIN3032078 ELISA WB Rabbit Ig Fraction AA 432-461 Log in to see Polyclonal 0
10.843901 ABIN2917954 IF/ICC IHC IP WB Rabbit AA 134-393 Log in to see Polyclonal 0
10.843901 ABIN2430575 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
7.8439007 ABIN388619 WB Rabbit Ig Fraction AA 432-461, C-Term Log in to see Polyclonal 0
7.8439007 ABIN2995223 WB Rabbit IgG Log in to see Polyclonal 0
7.8439007 ABIN2423911 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN357219 EIA WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 4
7 ABIN5531720 WB Rabbit Ig Fraction AA 432-461, C-Term Log in to see Polyclonal 0
4.8439007 ABIN2784862 IF WB Rabbit C-Term Log in to see Polyclonal 1
4.8439007 ABIN2784861 WB Rabbit N-Term Log in to see Polyclonal 0
4.8439007 ABIN5996047 WB Rabbit IgG Log in to see Polyclonal 0
4.8439007 ABIN2564195 IHC ELISA WB Goat IgG C-Term Log in to see Polyclonal 0
4 ABIN962675 ELISA IHC IHC (p) WB Goat AA 429-440 Log in to see Polyclonal 0
4 ABIN1737602 IHC (p) ELISA WB Goat C-Term Log in to see Polyclonal 0
1.6847457 ABIN4341182 ICC IF IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN5552858 IHC (p) WB Goat C-Term Log in to see Polyclonal 0
1 ABIN2581697 WB Rabbit IgG AA 134-393 Log in to see Polyclonal 0
1 ABIN4904600 WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1919047 IF IHC IHC (p) IP WB Rabbit IgG AA 418-468 Log in to see Polyclonal 0

General

Antigen Nucleoporin 50kDa (NUP50) Antibodies
Epitope C-Term
(21), (11), (9), (8), (7), (5), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(107), (40), (29), (9), (3), (3), (3), (2), (2), (1), (1)
Host Rabbit
(74), (19), (19)
Clonality
Polyclonal
Conjugate This NUP50 antibody is un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(93), (55), (20), (13), (12), (9), (5), (4), (3), (2)
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Product Details anti-NUP50 Antibody

Target Details NUP50 Application Details Handling Images
Cross-Reactivity Xenopus laevis, Xenopus tropicalis, Chicken, Rhesus Monkey, Cow (Bovine)
Cross-Reactivity (Details) Xenopus laevis (91 %), Xenopus Tropicalis (91 %), Chicken (92 %), Rhesus Monkey (100 %), Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to NUP50 (nucleoporin 50 kDa)
NUP50 antibody [C3], C-term
Purification Purified by antigen-affinity chromatography.
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NUP50. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details NUP50

Product Details anti-NUP50 Antibody Application Details Handling Images back to top
Antigen
Alternative Name NUP50 (NUP50 Antibody Abstract)
Background The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Nucleus , nuclear pore complex , Nucleus membrane, Peripheral membrane protein, Nucleoplasmic side (By similarity)
Molecular Weight 50 kDa
Gene ID 10762
Pathways Tube Formation

Application Details

Product Details anti-NUP50 Antibody Target Details NUP50 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: H1299 , mouse testis

Restrictions For Research Use only

Handling

Product Details anti-NUP50 Antibody Target Details NUP50 Application Details Images back to top
Format Liquid
Concentration 0.79 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-NUP50 Antibody Target Details NUP50 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Nucleoporin 50kDa (NUP50) (C-Term) antibody (ABIN2856374) IHC-P Image Immunohistochemical analysis of paraffin-embedded human normal colon, usi...
Western Blotting (WB) image for anti-Nucleoporin 50kDa (NUP50) (C-Term) antibody (ABIN2856374) WB Image Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE antibody diluted a...
Western Blotting (WB) image for anti-Nucleoporin 50kDa (NUP50) (C-Term) antibody (ABIN2856374) WB Image NUP50 antibody [C3], C-term detects NUP50 protein by Western blot analysis. ...
Immunofluorescence (IF) image for anti-Nucleoporin 50kDa (NUP50) (C-Term) antibody (ABIN2856374) ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde...