anti-Human CAMK2A antibody for Immunohistochemistry

Recommended CAMK2A Antibody (supplied by: Log in to see )

Antigen
Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies
  • camk2
  • camk2b
  • zgc:112538
  • zgc:123320
  • CAMKA
  • CaMKII
  • R74975
  • mKIAA0968
  • PK2CDD
  • PKCCD
  • calcium/calmodulin dependent protein kinase II alpha
  • calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog
  • calcium/calmodulin-dependent protein kinase II alpha
  • CAMK2A
  • camk2a.S
  • camk2a
  • Camk2a
Epitope
Internal Region
27
25
13
11
10
9
6
6
5
5
4
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
144
91
90
26
8
7
5
4
3
3
2
1
1
1
1
1
1
1
1
Host
Goat
117
58
19
Clonality
Polyclonal
Conjugate
This CAMK2A antibody is un-conjugated
7
7
7
6
6
6
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
191
99
72
56
46
36
27
10
7
6
4
2
2
1
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Catalog No. ABIN570984
$ 429.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.863952 ABIN574315 ELISA IHC IHC (p) WB Goat AA 455-468 Log in to see Polyclonal 0
12.863952 ABIN3003199 IHC WB Rabbit IgG Log in to see Polyclonal 0
12.863952 ABIN3003046 IHC WB Rabbit IgG Log in to see Polyclonal 0
12.38187 ABIN5619026 ICC IF IHC WB Rabbit Center Log in to see Polyclonal 0
9.863952 ABIN214329 ELISA IF IHC IHC (p) WB Rabbit AA 6-23 Log in to see Polyclonal 0
9.863952 ABIN2464145 IHC ELISA WB Goat Internal Region Log in to see Polyclonal 0
4 ABIN1871411 IHC WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN5970216 IHC WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN2561549 IHC ELISA WB Goat IgG Internal Region Log in to see Polyclonal 0
1 ABIN2217779 IHC ELISA WB Goat Internal Region Log in to see Polyclonal 0
1 ABIN227220 IHC IHC (fro) WB Rabbit pThr305 Log in to see Polyclonal 0
1 ABIN227221 IHC IHC (fro) WB Rabbit pThr286 Log in to see Polyclonal 0
1 ABIN200353 ELISA IHC IHC (p) IP WB Mouse IgG2a, kappa Log in to see 0
1 ABIN2893425 IHC IHC (p) WB Rabbit Lys292 Log in to see Polyclonal 0
1 ABIN2217787 IHC ELISA WB Rabbit IgG C-Term, Glu370 Log in to see Polyclonal 0
1 ABIN1963812 IHC ELISA WB APC Rabbit IgG AA 446-478 Log in to see Polyclonal 0
1 ABIN1966447 IHC ELISA WB Biotin Rabbit IgG AA 446-478 Log in to see Polyclonal 0
1 ABIN1970254 IHC ELISA WB FITC Rabbit IgG AA 446-478 Log in to see Polyclonal 0
1 ABIN1961435 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 446-478 Log in to see Polyclonal 0
1 ABIN1974832 IHC ELISA WB PE Rabbit IgG AA 446-478 Log in to see Polyclonal 0

General

Antigen Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies
Epitope Internal Region
(27), (25), (13), (11), (10), (9), (6), (6), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(144), (91), (90), (26), (8), (7), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Goat
(117), (58), (19)
Clonality
Polyclonal
Conjugate This CAMK2A antibody is un-conjugated
(7), (7), (7), (6), (6), (6), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(191), (99), (72), (56), (46), (36), (27), (10), (7), (6), (4), (2), (2), (1)
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Product Details anti-CAMK2A Antibody

Target Details CAMK2A Application Details Handling Images
Purpose CAMK2A
Blocking Peptide Blocking peptide for this product available: ABIN2815316
Sequence PRTAQSEETR VWHR
Specificity This antibody is expected to recognize both reported isoforms (NP_057065.2, NP_741960.1).
Predicted Reactivity Human, Mouse, Rat
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-PRTAQSEETRVWHR, from the internal region of the protein sequence according to NP_057065.2, NP_741960.1.
Plasmids, Primers & others

Target Details CAMK2A

Product Details anti-CAMK2A Antibody Application Details Handling Images back to top
Antigen
Alternative Name CAMK2A (CAMK2A Antibody Abstract)
Background CAMK2A, calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, CAMKA, KIAA0968, CaM kinase II alpha subunit, CaM-kinase II alpha chain, CaMK-II alpha subunit, OTTHUMP00000165787, OTTHUMP00000165788, calcium/calmodulin-dependent protein kinase II alpha-B subunit, calcium/calmodulin-dependent protein kinase IIA, calcium/calmodulin-dependent protein kinase type II alpha chain
Gene ID 815
NCBI Accession NP_057065, NP_741960
Pathways WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction

Application Details

Product Details anti-CAMK2A Antibody Target Details CAMK2A Handling Images back to top
Application Notes

DS WB Results: Approx 58-60 kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 55.3 kDa according to Human NP_057065.2, and 54.1 kDa according to to Mouse NP_803126.1 and Rat NP_037052.1). An additional band of 50 kDa was observed in some Rat Brain lysates and wss successfully blocked by incubation with the immunizing peptide. The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03--0.3 μg/mL. Primary incubation 1 hour at room temperature.

DS IHC Results: In paraffin embedded Human Brain Cortex shows granular staining in the periphery of pyramydal cell bodies. Recommended concentration: 3-6 μg/mL. Paraffin embedded Human Adrenal Gland. Recommended concentration: 3.75 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:64000.

Comment

Immunofluorescence: Strong expression of the protein seen in the membranes of U2OS and U251 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.

Restrictions For Research Use only

Handling

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

Images

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (Internal Region) antibody (ABIN570984) Antibody (0.3µg/ml) staining of Human Cerebellum (A), Mouse (B) and Rat Brain (C) lys...