anti-Cow (Bovine) CTNNB1 antibody for Immunocytochemistry

Recommended CTNNB1 Antibody (supplied by: Log in to see )

Antigen
Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) Antibodies
  • Jup
  • LOC100217813
  • CTNNB1
  • CTNNB
  • Bfc
  • Catnb
  • Mesc
  • ctnnb
  • id:ibd2058
  • wu:fb73e10
  • wu:fi81c06
  • wu:fk25h01
  • ctnnb1
  • CHBCAT
  • MRD19
  • armadillo
  • beta-catenin
  • catenin beta 1
  • catenin (cadherin associated protein), beta 1
  • catenin (cadherin-associated protein), beta 1
  • catenin beta 1 S homeolog
  • catenin beta 1 L homeolog
  • CTNNB1
  • Ctnnb1
  • ctnnb1
  • ctnnb1.S
  • ctnnb1.L
Epitope
AA 29-49
77
20
19
18
17
17
15
15
14
10
10
8
8
8
6
6
5
5
5
5
5
5
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Bat, Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
519
235
210
60
50
38
25
24
16
16
13
12
10
8
6
6
3
1
Host
Rabbit
336
197
8
2
Clonality
Polyclonal
Conjugate
This CTNNB1 antibody is un-conjugated
14
11
8
7
7
6
4
4
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
489
208
175
140
116
108
67
28
23
13
11
8
4
3
3
2
2
1
Supplier
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Catalog No. ABIN211079
$ 1,035.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2740182 ICC IF IHC IHC (p) WB Rabbit pSer33 Log in to see Polyclonal 0
1 ABIN2619645 ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN2619648 ICC IF IHC IHC (p) WB Rabbit pSer37 Log in to see Polyclonal 0

General

Antigen Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) Antibodies
Epitope AA 29-49
(77), (20), (19), (18), (17), (17), (15), (15), (14), (10), (10), (8), (8), (8), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bat, Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(519), (235), (210), (60), (50), (38), (25), (24), (16), (16), (13), (12), (10), (8), (6), (6), (3), (1)
Host Rabbit
(336), (197), (8), (2)
Clonality
Polyclonal
Conjugate This CTNNB1 antibody is un-conjugated
(14), (11), (8), (7), (7), (6), (4), (4), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
(489), (208), (175), (140), (116), (108), (67), (28), (23), (13), (11), (8), (4), (3), (3), (2), (2), (1)
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Product Details anti-CTNNB1 Antibody

Target Details CTNNB1 Application Details Handling Images
Specificity Recognizes b-Catenin, Mr 92kD. Species cross-reactivity: Human, bovine, rat, rabbit and mouse.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Pufferfish, Zebrafish (100%) Stickleback (90%).
Purification Protein A purified
Immunogen Synthetic peptide corresponding to the consensus GSK3 phosphorylation site (aa29-49, CGG-SYLDSGIHSGATTTAPSLSGK) of human b-Catenin (KLH coupled). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Pufferfish, Zebrafish (100%), Stickleback (90%).

Type of Immunogen: Synthetic peptide - KLH conjugated
Isotype IgG
Plasmids, Primers & others

Target Details CTNNB1

Product Details anti-CTNNB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CTNNB1 / Beta Catenin (CTNNB1 Antibody Abstract)
Background Name/Gene ID: CTNNB1
Family: Catenin

Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin
Gene ID 1499
UniProt P35222
Pathways WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2

Application Details

Product Details anti-CTNNB1 Antibody Target Details CTNNB1 Handling Images back to top
Application Notes Approved: ICC (1 - 5 μg/mL), IP, WB (0.1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 0.1-2 μg/mL. Detects b-catenin in an A431 total cell lysate. A431 total cell lysate was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-b-Catenin (0.1 μg/mL). Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg. Immunoprecipitates b-Catenin from 500 μg of A431 membrane fraction lysate. Immunocytochemistry: 1-5 μg/mL. Demonstrates positive immunostaining for b-catenin at cell-cell junctions of A431 cells fixed with 4 % paraformaldehyde and permeabilized with 0.25 % Triton X-100. Positive control: A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.