anti-Mouse (Murine) FZD3 antibody for Immunohistochemistry

Recommended FZD3 Antibody (supplied by: Log in to see )

Antigen
Frizzled Family Receptor 3 (FZD3) Antibodies
  • FZD3
  • fz3
  • Fz-3
  • Xfz3
  • frz3
  • hfz3
  • frz-3
  • frizzled3
  • frizzled-3
  • FZ-3
  • AU020229
  • D930050A07Rik
  • Fz3
  • frizzled class receptor 3
  • frizzled class receptor 3 L homeolog
  • FZD3
  • fzd3
  • Fzd3
  • fzd3.L
Epitope
N-Term
18
15
13
11
11
10
7
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Reactivity
Bat, Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
130
76
26
2
2
2
2
2
2
2
2
1
Host
Rabbit
91
22
16
7
Clonality
Polyclonal
Conjugate
This FZD3 antibody is un-conjugated
7
4
4
4
4
3
3
3
3
3
3
2
2
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
73
60
22
22
22
19
13
4
3
2
2
1
1
1
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Catalog No. ABIN1048620
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.289492 ABIN1048621 IHC IHC (p) Rabbit N-Term Log in to see Polyclonal 0
9.289492 ABIN2879282 ELISA IF IHC IHC (p) WB Rabbit IgG AA 481-530 Log in to see Polyclonal 0
9.289492 ABIN5693226 IHC WB Rabbit Log in to see Polyclonal 0
9.289492 ABIN2431915 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
9.289492 ABIN2426487 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
1 ABIN440985 IHC WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN4898967 CyTOF FACS IHC Rat IgG2a AA 1-157 Log in to see 169310 3
1 ABIN2854269 IHC WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN4898965 FACS IHC WB Biotin Rat IgG2a AA 1-157 Log in to see 169310 0
1 ABIN470848 FACS ICC IHC IHC (fro) WB Biotin Rat IgG2a Cysteine-Rich Domain, Extracellular Log in to see 0
1 ABIN470847 ELISA FACS IHC IHC (fro) WB Rat IgG2a Cysteine-Rich Domain, Extracellular Log in to see 0
1 ABIN301678 ICC IHC WB Goat IgG Cysteine-Rich Domain, Extracellular Log in to see Polyclonal 0
1 ABIN6039634 ELISA IHC Rabbit IgG Log in to see Polyclonal 0
1 ABIN6040288 ELISA IHC Rabbit IgG Log in to see Polyclonal 0
1 ABIN2269666 IC FACS IHC WB Biotin Rat IgG2a Log in to see 9j126 0
1 ABIN2269665 FACS IHC ELISA Rat IgG2a Log in to see 9j126 0
1 ABIN2269667 IC IHC ELISA WB Goat IgG Log in to see Polyclonal 0

General

Antigen Frizzled Family Receptor 3 (FZD3) Antibodies
Epitope N-Term
(18), (15), (13), (11), (11), (10), (7), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Bat, Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(130), (76), (26), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Host Rabbit
(91), (22), (16), (7)
Clonality
Polyclonal
Conjugate This FZD3 antibody is un-conjugated
(7), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(73), (60), (22), (22), (22), (19), (13), (4), (3), (2), (2), (1), (1), (1)
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Product Details anti-FZD3 Antibody

Target Details FZD3 Application Details Handling Images
Brand IHC-plus™
Specificity Human FZD3 / Frizzled 3. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FZD6 (39 % ).
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken (100%) Xenopus (89%) Platypus, Zebrafish (83%).
Purification Immunoaffinity purified
Immunogen Synthetic 18 amino acid peptide from N-terminal extracellular domain of human FZD3 / Frizzled 3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken (100%), Xenopus (89%), Platypus, Zebrafish (83%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details FZD3

Product Details anti-FZD3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name FZD3 / Frizzled 3 (FZD3 Antibody Abstract)
Background Name/Gene ID: FZD3
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD3, Frizzled family receptor 3, Frizzled 3, HFz3, Frizzled homolog 3, Fz-3, Wnt receptor frizzled-3, Frizzled-3
Gene ID 7976
Pathways WNT Signaling, Tube Formation

Application Details

Product Details anti-FZD3 Antibody Target Details FZD3 Handling Images back to top
Application Notes Approved: IHC, IHC-P (3 - 5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3-5 μg/mL.
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-FZD3 Antibody Target Details FZD3 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-FZD3 Antibody Target Details FZD3 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Frizzled Family Receptor 3 (FZD3) (N-Term) antibody (ABIN1048620) Anti-FZD3 / Frizzled 3 antibody ABIN1048620 IHC staining of human skeletal muscle. I...
Immunohistochemistry (IHC) image for anti-Frizzled Family Receptor 3 (FZD3) (N-Term) antibody (ABIN1048620) Anti-FZD3 / Frizzled 3 antibody ABIN1048620 IHC staining of human skin. Immunohistoc...