anti-Mouse (Murine) FZD4 antibody for Immunocytochemistry

Recommended FZD4 Antibody

Antigen
Frizzled Family Receptor 4 (FZD4) Antibodies
  • CG4626
  • DFz4
  • Dfz4
  • Dm Fz4
  • Dmel\\CG4626
  • Fz4
  • anon-WO0170980.10
  • anon-WO0170980.11
  • fz1
  • zg01
  • CD344
  • EVR1
  • FEVR
  • FZD4S
  • Fz-4
  • FzE4
  • GPCR
  • hFz4
  • frizzled4
  • fz4
  • FZ-4
  • frizzled 4
  • frizzled class receptor 4
  • frizzled-4
  • frizzled class receptor 4 S homeolog
  • fz4
  • fzd4
  • Tsp_10376
  • FZD4
  • Fzd4
  • fzd4.S
Epitope
AA 1-180
42
24
16
15
10
8
7
4
4
4
4
3
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
153
113
58
6
6
5
5
5
4
4
3
3
3
2
1
Host
Rat
143
27
5
4
Clonality
Monoclonal
Conjugate
This FZD4 antibody is conjugated to Biotin
12
7
7
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
123
68
45
16
13
12
6
4
2
2
1
1
Options
Catalog No. ABIN4898976
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
5.5 ABIN4900304 CyTOF FACS ICC WB Rat IgG2a AA 37-180 145901 2
1 ABIN6757418 ICC FACS WB Rat IgG2a Extracellular Domain 0
1 ABIN478754 ICC IHC ELISA WB Goat IgG Cysteine-Rich Domain, Extracellular Polyclonal 0

General

Antigen Frizzled Family Receptor 4 (FZD4) Antibodies
Epitope AA 1-180
(42), (24), (16), (15), (10), (8), (7), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(153), (113), (58), (6), (6), (5), (5), (5), (4), (4), (3), (3), (3), (2), (1)
Host Rat
(143), (27), (5), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FZD4 antibody is conjugated to Biotin
(12), (7), (7), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
(123), (68), (45), (16), (13), (12), (6), (4), (2), (2), (1), (1)
Pubmed 1 reference available
Supplier

Product Details anti-FZD4 Antibody

Target Details FZD4 Application Details Handling References for anti-FZD4 antibody (ABIN4898976) Images
Specificity Detects human and mouse Frizzled-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse Frizzled-1, -2, -3, -6, -7, or -8 is observed. In flow cytometry and immunohistochemistry, this antibody also detected Frizzled-4 on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3, and also on human HEK293 cells.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.15 EU per 1 μg of the antibody by the LAL method.
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4, Met1-Glu180, Accession # Q61088
Clone 145901
Isotype IgG2a
Plasmids, Primers & others

Target Details FZD4

Product Details anti-FZD4 Antibody Application Details Handling References for anti-FZD4 antibody (ABIN4898976) Images back to top
Antigen
Alternative Name Frizzled-4 (FZD4 Antibody Abstract)
Background Alternative Names: CD344, CD344 antigen, EVR1, exudative vitreoretinopathy 1, FEVR, frizzled (Drosophila) homolog 4, frizzled homolog 4 (Drosophila), frizzled-4, Frizzled4, Fz-4, FZD4, FZD4S, FzE4, GPCR, hFz4, MGC34390, WNT receptor frizzled-4
Gene ID 8322
UniProt Q61088
Pathways WNT Signaling, Hormone Transport, Sensory Perception of Sound

Application Details

Product Details anti-FZD4 Antibody Target Details FZD4 Handling References for anti-FZD4 antibody (ABIN4898976) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-FZD4 Antibody Target Details FZD4 Application Details References for anti-FZD4 antibody (ABIN4898976) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-FZD4 antibody (ABIN4898976)

Product Details anti-FZD4 Antibody Target Details FZD4 Application Details Handling Images back to top
Product cited in:

Despeaux, Chicanne, Rouer, De Toni-Costes, Bertrand, Mansat-De Mas, Vergnolle, Eaves, Payrastre, Girault, Racaud-Sultan: "Focal adhesion kinase splice variants maintain primitive acute myeloid leukemia cells through altered Wnt signaling." in: Stem cells (Dayton, Ohio), Vol. 30, Issue 8, pp. 1597-610, 2012 Method employed by authors: Flow Cytometry (FACS), Flow Cytometry (FACS), Flow Cytometry (FACS) (Sample species: Human).