anti-Rat (Rattus) FZD4 antibody for Western Blotting

Recommended FZD4 Antibody (supplied by: Log in to see )

Antigen
Frizzled Family Receptor 4 (FZD4) Antibodies
  • CG4626
  • DFz4
  • Dfz4
  • Dm Fz4
  • Dmel\\CG4626
  • Fz4
  • anon-WO0170980.10
  • anon-WO0170980.11
  • fz1
  • zg01
  • CD344
  • EVR1
  • FEVR
  • FZD4S
  • Fz-4
  • FzE4
  • GPCR
  • hFz4
  • frizzled4
  • fz4
  • FZ-4
  • frizzled 4
  • frizzled class receptor 4
  • frizzled-4
  • frizzled class receptor 4 S homeolog
  • fz4
  • fzd4
  • Tsp_10376
  • FZD4
  • Fzd4
  • fzd4.S
Epitope
Middle Region
36
26
16
15
11
8
8
4
4
4
4
3
3
3
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
138
109
58
7
7
7
7
5
5
5
5
4
2
2
2
1
Host
Rabbit
131
26
15
8
Clonality
Polyclonal
Conjugate
This FZD4 antibody is un-conjugated
11
8
7
7
6
5
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
117
67
19
18
18
13
13
6
5
2
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN2776715
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1348808 ABIN2706181 WB Rabbit Center Log in to see Polyclonal 0
3.1348808 ABIN2706182 WB Rabbit Center Log in to see Polyclonal 0
3.1348808 ABIN2707513 WB Rabbit N-Term Log in to see Polyclonal 0
3.1348808 ABIN5693200 IHC WB Rabbit Log in to see Polyclonal 0
3.1348808 ABIN2736776 WB Rabbit IgG Log in to see Polyclonal 0
3.1348808 ABIN2458568 ELISA WB Rabbit Log in to see Polyclonal 0
3.1348808 ABIN2971917 WB Rabbit N-Term Log in to see Polyclonal 0
3.1348808 ABIN2562681 ELISA WB Goat IgG Internal Region Log in to see Polyclonal 0
3.1348808 ABIN2422355 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
3.1348808 ABIN2428931 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN768861 IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN5553432 WB Goat Internal Region Log in to see Polyclonal 0
1 ABIN4901562 ELISA WB Rabbit IgG AA 11-60 Log in to see Polyclonal 0
1 ABIN1714167 IF (p) IHC (p) WB Rabbit IgG AA 190-222 Log in to see Polyclonal 0
1 ABIN1711682 IHC (p) WB HRP Rabbit IgG AA 190-222 Log in to see Polyclonal 0
1 ABIN1700665 IHC (p) WB Biotin Rabbit IgG AA 190-222 Log in to see Polyclonal 0
1 ABIN2621124 WB Rabbit Internal Region Log in to see Polyclonal 0
1 ABIN2621125 WB Rabbit Internal Region Log in to see Polyclonal 0
1 ABIN2741013 WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN1836957 WB Rabbit AA 280-329 Log in to see Polyclonal 0

General

Antigen Frizzled Family Receptor 4 (FZD4) Antibodies
Epitope Middle Region
(36), (26), (16), (15), (11), (8), (8), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Cow (Bovine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
(138), (109), (58), (7), (7), (7), (7), (5), (5), (5), (5), (4), (2), (2), (2), (1)
Host Rabbit
(131), (26), (15), (8)
Clonality
Polyclonal
Conjugate This FZD4 antibody is un-conjugated
(11), (8), (7), (7), (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(117), (67), (19), (18), (18), (13), (13), (6), (5), (2), (1), (1), (1), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-FZD4 Antibody

Target Details FZD4 Application Details Handling References for anti-FZD4 antibody (ABIN2776715) Images
Blocking Peptide Blocking peptide for this product available: ABIN977169
Sequence GITSGMWIWS AKTLHTWQKC SNRLVNSGKV KREKRGNGWV KPGKGSETVV
Predicted Reactivity Cow: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against FZD4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human FZD4
Plasmids, Primers & others

Target Details FZD4

Product Details anti-FZD4 Antibody Application Details Handling References for anti-FZD4 antibody (ABIN2776715) Images back to top
Antigen
Alternative Name FZD4 (FZD4 Antibody Abstract)
Background FZD4 is a member of the frizzled family. Members of this family are seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence.This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: EVR1, FEVR, FZD4S, Fz-4, FzE4, GPCR, MGC34390, CD344
Protein Interaction Partner: UBC, NDP, ZNRF3, BAG6, MAGI3, DLG2, DLG4, DVL2, DLG1, ARRB2,
Protein Size: 537
Molecular Weight 60 kDa
Gene ID 8322
NCBI Accession NM_012193, NP_036325
UniProt Q9ULV1
Pathways WNT Signaling, Hormone Transport, Sensory Perception of Sound

Application Details

Product Details anti-FZD4 Antibody Target Details FZD4 Handling References for anti-FZD4 antibody (ABIN2776715) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 537 AA

Restrictions For Research Use only

Handling

Product Details anti-FZD4 Antibody Target Details FZD4 Application Details References for anti-FZD4 antibody (ABIN2776715) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FZD4 antibody (ABIN2776715)

Product Details anti-FZD4 Antibody Target Details FZD4 Application Details Handling Images back to top
Product cited in:

Wang, Pawlak, Johnson, Belknap, Eades, Stack, Cousin, Black: "Impact of laminitis on the canonical Wnt signaling pathway in basal epithelial cells of the equine digital laminae." in: PLoS ONE, Vol. 8, Issue 2, pp. e56025, 2013

McIver, Stanger, Santarelli, Roman, Nixon, McLaughlin: "A unique combination of male germ cell miRNAs coordinates gonocyte differentiation." in: PLoS ONE, Vol. 7, Issue 4, pp. e35553, 2012

Gonzalez, Fernandez-Martos, Gonzalez-Fernandez, Arenas, Rodriguez: "Spatio-temporal expression pattern of frizzled receptors after contusive spinal cord injury in adult rats." in: PLoS ONE, Vol. 7, Issue 12, pp. e50793, 2012

Background publications

Dufourcq, Leroux, Ezan, Descamps, Lamazière, Costet, Basoni, Moreau, Deutsch, Couffinhal, Duplàa: "Regulation of endothelial cell cytoskeletal reorganization by a secreted frizzled-related protein-1 and frizzled 4- and frizzled 7-dependent pathway: role in neovessel formation." in: The American journal of pathology, Vol. 172, Issue 1, pp. 37-49, 2008

Images

Product Details anti-FZD4 Antibody Target Details FZD4 Application Details Handling References for anti-FZD4 antibody (ABIN2776715) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Frizzled Family Receptor 4 (FZD4) (Middle Region) antibody (ABIN2776715) anti-Frizzled Family Receptor 4 (FZD4) (Middle Region) antibody
Western Blotting (WB) image for anti-Frizzled Family Receptor 4 (FZD4) (Middle Region) antibody (ABIN2776715) WB Suggested Anti-FZD4 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positi...
Western Blotting (WB) image for anti-Frizzled Family Receptor 4 (FZD4) (Middle Region) antibody (ABIN2776715) Host: Rabbit Target Name: FZD4 Sample Tissue: Human Lung Tumor Antibody Dilutio...
Western Blotting (WB) image for anti-Frizzled Family Receptor 4 (FZD4) (Middle Region) antibody (ABIN2776715) Host: Rabbit Target Name: FZD4 Sample Tissue: Human Fetal Liver Antibody Diluti...