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|Antigen||Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) Antibodies|
|Conjugate||This IGFBP5 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-IGFBP5 AntibodyTarget Details IGFBP5 Application Details Handling Images
|Specificity||This antibody detects Insulin-like Growth Factor Binding Protein 5.|
|Immunogen||Highly pure (> 98 %) recombinant human IGF-BP5|
Target Details IGFBP5Product Details anti-IGFBP5 Antibody Application Details Handling Images back to top
|Alternative Name||IGFBP5 (IGFBP5 Antibody Abstract)|
|Background||IGFBP5 is one of a family of proteins that bind to insulin-like growth factors (IGFs). IGFBP5 is a multifunctional protein which acts not only as a traditional binding protein but also functions as a growth factor independent of IGFs to stimulate bone formation. Currently there are seven named IGF-BPs that form high affinity complexes with both IGFI and IGFII. IGFBP5 is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGFI on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGFBP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGFBP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development.Synonyms: IBP-5, IBP5, IGF-binding protein 5, IGFBP-5, Insulin-like growth factor-binding protein 5|
|Pathways||WNT Signaling, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding|
Application DetailsProduct Details anti-IGFBP5 Antibody Target Details IGFBP5 Handling Images back to top
Indirect ELISA: To detect hIGF-BP5 (using 100 μL/well antibody solution) a concentration of0.5-2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP5. Sandwich ELISA: To detect hIGF-BP5 (using 100 μL/well antibody solution) a concentrationof 0.5-2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanIGF-BP5 (ABIN181653 or ABIN181654) as a detection antibody, it allows the detection ofat least 0.2-0.4 ng/well of recombinant hIGF-BP5. Western blot: To detect hIGF-BP5 this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit forrecombinant hIGF-BP5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IGFBP5 Antibody Target Details IGFBP5 Application Details Images back to top
|Reconstitution||Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.|
|Expiry Date||6 months|
ImagesProduct Details anti-IGFBP5 Antibody Target Details IGFBP5 Application Details Handling back to top