anti-Guinea Pig LEF1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended LEF1 Antibody (supplied by: Log in to see )

Antigen
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
  • LEF1
  • 3000002B05
  • AI451430
  • Lef-1
  • LEF-1
  • TCF10
  • TCF1ALPHA
  • TCF7L3
  • lymphoid enhancer binding factor 1
  • lymphoid enhancer-binding factor 1
  • lymphoid enhancer factor XLEF-1B
  • LEF1
  • lef1
  • Lef1
Epitope
AA 51-100
15
13
13
9
8
6
6
6
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Human, Monkey
127
40
30
10
6
6
5
5
4
4
4
3
3
2
1
1
Host
Rabbit
55
52
16
5
Clonality
Polyclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
117
68
23
18
8
7
4
3
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN214792
$ 727.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN202428 IHC (p) WB Rabbit IgG AA 282-331 Log in to see Polyclonal 0

General

Antigen Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
Epitope AA 51-100
(15), (13), (13), (9), (8), (6), (6), (6), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Human, Monkey
(127), (40), (30), (10), (6), (6), (5), (5), (4), (4), (4), (3), (3), (2), (1), (1)
Host Rabbit
(55), (52), (16), (5)
Clonality
Polyclonal
Conjugate This LEF1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(117), (68), (23), (18), (8), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-LEF1 Antibody

Target Details LEF1 Application Details Handling Images
Brand IHC-plus™
Specificity Human LEF1
Predicted Reactivity Percent identity by BLAST analysis: Human, Dog, Bovine (100%) Pig (92%) Guinea pig (85%).
Purification Immunoaffinity purified
Immunogen Synthetic peptide located between aa51-100 of human LEF1 (Q9UJU2, NP_057353). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bovine (100%), Galago, Rabbit, Pig, Opossum (92%), Guinea pig (85%), Mouse, Rat (78%).

Type of Immunogen: Synthetic peptide

Target Details LEF1

Product Details anti-LEF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Name/Gene ID: LEF1

Synonyms: LEF1, LEF-1, TCF7L3, TCF1-alpha, TCF10, TCF1ALPHA
Gene ID 51176
NCBI Accession NP_057353
UniProt Q9UJU2
Pathways WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding

Application Details

Product Details anti-LEF1 Antibody Target Details LEF1 Handling Images back to top
Application Notes Approved: IHC-P (5 μg/mL), WB (0.1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.
Restrictions For Research Use only

Handling

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Images back to top
Format Lyophilized
Reconstitution Reconstitute with 50 μL sterile ddH2O.
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.

Images

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 51-100) antibody (ABIN214792) Human Prostate (formalin-fixed, paraffin-embedded) stained with LEF1 antibody ABIN214...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 51-100) antibody (ABIN214792) Human Tonsil (formalin-fixed, paraffin-embedded) stained with LEF1 antibody ABIN21479...