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|Antigen||Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies|
|Epitope||AA 51-100 Alternatives|
|Reactivity||Cow (Bovine), Dog (Canine), Guinea Pig, Human, Monkey Alternatives|
|Conjugate||This LEF1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-LEF1 AntibodyTarget Details LEF1 Application Details Handling Images
|Predicted Reactivity||Percent identity by BLAST analysis: Human, Dog, Bovine (100%) Pig (92%) Guinea pig (85%).|
Synthetic peptide located between aa51-100 of human LEF1 (Q9UJU2, NP_057353). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bovine (100%), Galago, Rabbit, Pig, Opossum (92%), Guinea pig (85%), Mouse, Rat (78%).
Type of Immunogen: Synthetic peptide
Target Details LEF1Product Details anti-LEF1 Antibody Application Details Handling Images back to top
|Alternative Name||LEF1 (LEF1 Antibody Abstract)|
Name/Gene ID: LEF1
Synonyms: LEF1, LEF-1, TCF7L3, TCF1-alpha, TCF10, TCF1ALPHA
|Research Area||Transcription Factors, Wnt, Lineage Markers, Wnt Signaling|
|Pathways||WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding|
Application DetailsProduct Details anti-LEF1 Antibody Target Details LEF1 Handling Images back to top
Approved: IHC-P (5 μg/mL), WB (0.1 - 2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.
|Restrictions||For Research Use only|
HandlingProduct Details anti-LEF1 Antibody Target Details LEF1 Application Details Images back to top
|Reconstitution||Reconstitute with 50 μL sterile ddH2O.|
|Buffer||Lyophilized from PBS with 2 % sucrose|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
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