anti-Mouse (Murine) LEF1 antibody for Immunohistochemistry

Recommended LEF1 Antibody (supplied by: Log in to see )

Antigen
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
  • LEF1
  • 3000002B05
  • AI451430
  • Lef-1
  • LEF-1
  • TCF10
  • TCF1ALPHA
  • TCF7L3
  • lymphoid enhancer binding factor 1
  • lymphoid enhancer-binding factor 1
  • lymphoid enhancer factor XLEF-1B
  • LEF1
  • lef1
  • Lef1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
146
51
35
11
7
7
7
5
5
4
4
4
3
2
1
1
Host
Rabbit
67
58
17
5
Clonality
Polyclonal
Conjugate
This LEF1 antibody is un-conjugated
4
4
4
3
3
3
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
133
80
28
24
9
9
5
5
3
2
2
2
1
1
Supplier
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Available images

Catalog No. ABIN6262946
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.992594 ABIN2779620 IHC WB Rabbit C-Term Log in to see Polyclonal 1
9.992594 ABIN2428345 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
7 ABIN202428 IHC IHC (p) WB Rabbit IgG AA 282-331 Log in to see Polyclonal 0
6.992594 ABIN2421791 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
4 ABIN793575 ICC IHC WB Mouse IgG1 Log in to see REMB6 0
4 ABIN2460458 IHC ELISA WB Rabbit Log in to see Polyclonal 0
4 ABIN5582463 IHC WB Mouse IgG1 Log in to see REMB1 0
4 ABIN5582464 GS IHC WB Mouse IgG1 Log in to see REMB6 0
1 ABIN5701040 ELISA IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6089755 ELISA IF/ICC IHC WB Rabbit IgG AA 75-216 Log in to see Polyclonal 0
1 ABIN2938009 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6041876 ELISA IHC Rabbit IgG Log in to see Polyclonal 0
1 ABIN6041877 ELISA IHC Rabbit IgG Log in to see Polyclonal 0
1 ABIN1088251 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5902647 ELISA IHC IP WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(146), (51), (35), (11), (7), (7), (7), (5), (5), (4), (4), (4), (3), (2), (1), (1)
Host Rabbit
(67), (58), (17), (5)
Clonality
Polyclonal
Conjugate This LEF1 antibody is un-conjugated
(4), (4), (4), (3), (3), (3)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(133), (80), (28), (24), (9), (9), (5), (5), (3), (2), (2), (2), (1), (1)
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Product Details anti-LEF1 Antibody

Target Details LEF1 Application Details Handling Images
Specificity LEF1 Antibody detects endogenous levels of total LEF1
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthesized peptide derived from human LEF1
Isotype IgG
Plasmids, Primers & others

Target Details LEF1

Product Details anti-LEF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background

Description: Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

Gene: LEF1

Molecular Weight 55 kDa
Gene ID 51176
UniProt Q9UJU2
Pathways WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding

Application Details

Product Details anti-LEF1 Antibody Target Details LEF1 Handling Images back to top
Application Notes WB 1:1000-3000 IHC 1:200, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) antibody (ABIN6262946) Western blot analysis of LEF1 using HT-29 whole cell lysates
Immunohistochemistry (IHC) image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) antibody (ABIN6262946) ABIN6277777 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyd...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) antibody (ABIN6262946) ABIN6277777 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilize...