anti-Rat (Rattus) Phospholipase C beta 4 antibody for Western Blotting

Recommended Phospholipase C beta 4 Antibody (supplied by: Log in to see )

Antigen
Phospholipase C beta 4 (PLCb4) Antibodies
  • ARCND2
  • PI-PLC
  • A930039J07Rik
  • AI854601
  • C230058B11Rik
  • Beta4aa
  • RATBETA4AA
  • phospholipase C beta 4
  • phospholipase C, beta 4
  • PLCB4
  • Plcb4
Epitope
AA 752-961
1
1
1
1
Reactivity
Fly (Calliphora), Human, Mouse (Murine), Rat (Rattus)
6
2
1
1
Host
Mouse
5
1
1
Clonality
Monoclonal
Application
Immunofluorescence (IF), Western Blotting (WB)
6
2
2
2
1
1
1
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Catalog No. ABIN968900
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3322737 ABIN968583 BI IF WB Mouse IgG1 AA 752-961 Log in to see 56-Phospholipase Cbeta4 4

General

Antigen Phospholipase C beta 4 (PLCb4) Antibodies
Epitope AA 752-961
(1), (1), (1), (1)
Reactivity Fly (Calliphora), Human, Mouse (Murine), Rat (Rattus)
(6), (2), (1), (1)
Host Mouse
(5), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Application Immunofluorescence (IF), Western Blotting (WB)
(6), (2), (2), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-Phospholipase C beta 4 Antibody

Target Details Phospholipase C beta 4 Application Details Handling References for anti-Phospholipase C beta 4 antibody (ABIN968900) Images
Cross-Reactivity Fruit Fly (Drosophila melanogaster), Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Phospholipase Cbeta4 aa. 752-961
Clone 56-Phospholipase Cbeta4
Isotype IgG1

Target Details Phospholipase C beta 4

Product Details anti-Phospholipase C beta 4 Antibody Application Details Handling References for anti-Phospholipase C beta 4 antibody (ABIN968900) Images back to top
Antigen
Alternative Name Phospholipase C beta 4 (PLCb4 Antibody Abstract)
Background Phospholipase C (PLC) hydrolyzes inositol phospholipids into diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Multiple distinct PLC isoenzymes have been identified and divided into three structural types: alpha, beta, and gamma. This classification is based primarily on the location of the conserved X and Y domains, whose structural integrity is essential for a functional catalytic core. The activation of PLCbeta isoenzymes is uniquely regulated by G protein subunits, while PLCgamma is activated following phosphorylation by protein tyrosine kinases. The beta subfamily of PLC consists of at least four members: beta1, beta2, beta3, and beta4. PLCbeta4 differs from the other members in that it is not activated by G protein betagamma subunits, it is not found in the liver or kidney, and it is inhibited by ribonucleotides. Various isoforms of PLbetaC4 result from alternative splicing or proteolytic cleavage. PLCbeta4 is expressed in retina and brain and knockout mice display ataxia and abnormalities in metabotropic glutamate receptor function in the cerebellum. Thus, PLCbeta4 is primarily found in neuronal tissues where it is thought to be important in neurotransmitter signaling pathways.
Synonyms: PLCbeta4
Molecular Weight 130 kDa
Pathways WNT Signaling, Thyroid Hormone Synthesis, G-protein mediated Events

Application Details

Product Details anti-Phospholipase C beta 4 Antibody Target Details Phospholipase C beta 4 Handling References for anti-Phospholipase C beta 4 antibody (ABIN968900) Images back to top
Application Notes The Drosophila melanogaster gene, no receptor potential A (abbreviated as norpA), encodes a 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase involved in calcium-mediated signaling, which is a component of the membrane fraction. Its amino acid sequence contains a phosphatidylinositol-specific phospholipase C (X domain), a phosphoinositide-specific phospholipase C (PLC) and a phosphatidylinositol-specific phospholipase C (Y domain). Similar sequences have been identified in C. elegans, Homo sapiens, Mus musculus, Rattus norvegicus, and Saccharomyces cerevisiae. It has been mapped by recombination to 1-6.5 and cytologically to 4C1.
Comment

Related Products: ABIN967389, ABIN968539, ABIN968583

Restrictions For Research Use only

Handling

Product Details anti-Phospholipase C beta 4 Antibody Target Details Phospholipase C beta 4 Application Details References for anti-Phospholipase C beta 4 antibody (ABIN968900) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-Phospholipase C beta 4 antibody (ABIN968900)

Product Details anti-Phospholipase C beta 4 Antibody Target Details Phospholipase C beta 4 Application Details Handling Images back to top
Product cited in:

Kano, Hashimoto, Watanabe, Kurihara, Offermanns, Jiang, Wu, Jun, Shin, Inoue, Simon, Wu: "Phospholipase cbeta4 is specifically involved in climbing fiber synapse elimination in the developing cerebellum." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 26, pp. 15724-9, 1999

Kim, Jun, Lee, Kang, Min, Kim, Ryu, Suh, Shin: "Phospholipase C isozymes selectively couple to specific neurotransmitter receptors." in: Nature, Vol. 389, Issue 6648, pp. 290-3, 1997

Alvarez, Ghalayini, Xu, Hardcastle, Bhattacharya, Rao, Pettenati, Anderson, Baehr: "cDNA sequence and gene locus of the human retinal phosphoinositide-specific phospholipase-C beta 4 (PLCB4)." in: Genomics, Vol. 29, Issue 1, pp. 53-61, 1996

Lee, Park, Lee, Kim, Rhee: "Purification, molecular cloning, and sequencing of phospholipase C-beta 4." in: The Journal of biological chemistry, Vol. 268, Issue 28, pp. 21318-27, 1993

Images

Product Details anti-Phospholipase C beta 4 Antibody Target Details Phospholipase C beta 4 Application Details Handling References for anti-Phospholipase C beta 4 antibody (ABIN968900) back to top
Supplier Images
Western Blotting (WB) image for anti-Phospholipase C beta 4 (PLCb4) (AA 752-961) antibody (ABIN968900) Western blot analysis of Phospholipase Cbeta4. Lysates from PC12 cells (Rat neuroblas...