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|Antigen||Protein Kinase C, alpha (PKCa) Antibodies|
|Conjugate||This PKC alpha antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-PKC alpha AntibodyTarget Details PKC alpha Application Details Handling References for anti-PKC alpha antibody (ABIN359058) Images
|Specificity||This antibody reacts to PKC alpha.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKC.|
Target Details PKC alphaProduct Details anti-PKC alpha Antibody Application Details Handling References for anti-PKC alpha antibody (ABIN359058) Images back to top
|Alternative Name||PRKCA (PKCa Antibody Abstract)|
|Background||Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC alpha is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.Synonyms: PKC alpha, PKC-A, PKC-alpha, PKCA, PRKACA, Protein kinase C alpha type|
|Gene ID||5578, 9606|
|Pathways||WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling|
Application DetailsProduct Details anti-PKC alpha Antibody Target Details PKC alpha Handling References for anti-PKC alpha antibody (ABIN359058) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PKC alpha Antibody Target Details PKC alpha Application Details References for anti-PKC alpha antibody (ABIN359058) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-PKC alpha antibody (ABIN359058)Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling Images back to top
Rabinovitz, Tsomo, Mercurio: "Protein kinase C-alpha phosphorylation of specific serines in the connecting segment of the beta 4 integrin regulates the dynamics of type II hemidesmosomes." in: Molecular and cellular biology, Vol. 24, Issue 10, pp. 4351-60, 2004
Gong, Wei, Chowdhury, Chatterjee: "Lactosylceramide recruits PKCalpha/epsilon and phospholipase A2 to stimulate PECAM-1 expression in human monocytes and adhesion to endothelial cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 17, pp. 6490-5, 2004
Hermoso, Olivero, Torres, Riveros, Quest, Stutzin: "Cell volume regulation in response to hypotonicity is impaired in HeLa cells expressing a protein kinase Calpha mutant lacking kinase activity." in: The Journal of biological chemistry, Vol. 279, Issue 17, pp. 17681-9, 2004
Lorenz, Lohse, Quitterer: "Protein kinase C switches the Raf kinase inhibitor from Raf-1 to GRK-2." in: Nature, Vol. 426, Issue 6966, pp. 574-9, 2003
Petroski, Deshaies: "Context of multiubiquitin chain attachment influences the rate of Sic1 degradation." in: Molecular cell, Vol. 11, Issue 6, pp. 1435-44, 2003
ImagesProduct Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling References for anti-PKC alpha antibody (ABIN359058) back to top