anti-Human PKC alpha antibody for ELISA

Recommended PKC alpha Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, alpha (PKCa) Antibodies
  • pkc-alpha
  • pkcalpha
  • im:7139045
  • si:dkey-8l19.1
  • LOC100219195
  • AAG6
  • PKC-alpha
  • PKCA
  • PRKACA
  • AI875142
  • Pkca
  • PKRCA
  • protein kinase C alpha
  • protein kinase C, alpha L homeolog
  • protein kinase C, alpha
  • PRKCA
  • prkca.L
  • prkca
  • Prkca
Epitope
AA 563-673
36
20
18
17
16
16
15
14
12
9
7
6
5
5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
305
219
175
31
26
25
21
21
20
5
3
3
2
1
1
1
1
Host
Mouse
274
43
2
Clonality
Monoclonal
Conjugate
This PKC alpha antibody is un-conjugated
14
13
10
7
6
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
259
150
135
69
55
49
32
32
26
23
13
5
4
4
2
1
1
1
Options
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Catalog No. ABIN393606
$ 461.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.164179 ABIN562379 IF IHC (p) PLA ELISA WB Mouse IgG1 kappa AA 563-672, partial Log in to see 2F11 0
1 ABIN153146 ICC FACS IHC (fro) IF IHC (p) IP IHC RIA ELISA WB Mouse IgG2a Log in to see MC5 14
1 ABIN1531462 IHC ELISA WB Rabbit IgG AA 606-655, pThr638 Log in to see Polyclonal 1
1 ABIN1532477 IHC ELISA WB Rabbit IgG AA 606-655 Log in to see Polyclonal 0
1 ABIN1531505 ELISA WB Rabbit IgG AA 623-672, pTyr657 Log in to see Polyclonal 0
1 ABIN1532520 ELISA WB Rabbit IgG AA 623-672 Log in to see Polyclonal 0
1 ABIN1533205 IHC ELISA Rabbit IgG AA 623-672 Log in to see Polyclonal 0
1 ABIN3182236 ELISA WB Rabbit IgG pTyr657 Log in to see Polyclonal 0
1 ABIN3182587 ELISA IHC WB Rabbit IgG pThr638 Log in to see Polyclonal 0
1 ABIN3186466 ELISA IHC WB Rabbit IgG Thr287 Log in to see Polyclonal 0
1 ABIN6271956 ELISA ICC IF WB Rabbit IgG pSer657 Log in to see Polyclonal 1
1 ABIN2888746 ELISA IHC IHC (p) WB Rabbit IgG AA 606-655 Log in to see Polyclonal 0
1 ABIN2888770 ELISA WB Rabbit IgG AA 623-672 Log in to see Polyclonal 0
1 ABIN214666 ELISA IHC IHC (p) Rabbit pThr497 Log in to see Polyclonal 0
1 ABIN598889 IF ELISA WB Mouse IgG1, kappa AA 563-673 Log in to see 2F11 0
1 ABIN3186465 ELISA WB Rabbit IgG Thr286 Log in to see Polyclonal 0
1 ABIN3186467 ELISA IHC Rabbit IgG Thr288 Log in to see Polyclonal 0
1 ABIN1456845 ELISA WB Rabbit IgG AA 604-653 Log in to see Polyclonal 0
1 ABIN5774310 ELISA WB Rabbit IgG pTyr657 Log in to see Polyclonal 0
1 ABIN6269891 ELISA IHC WB Rabbit IgG pTyr365 Log in to see Polyclonal 0

General

Antigen Protein Kinase C, alpha (PKCa) Antibodies
Epitope AA 563-673
(36), (20), (18), (17), (16), (16), (15), (14), (12), (9), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(305), (219), (175), (31), (26), (25), (21), (21), (20), (5), (3), (3), (2), (1), (1), (1), (1)
Host Mouse
(274), (43), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PKC alpha antibody is un-conjugated
(14), (13), (10), (7), (6), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(259), (150), (135), (69), (55), (49), (32), (32), (26), (23), (13), (5), (4), (4), (2), (1), (1), (1)
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Product Details anti-PKC alpha Antibody

Target Details PKC alpha Application Details Handling Images
Cross-Reactivity (Details) Human: ~~ Positive
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen

Antigen Type: Recombinant Protein

Target/Specificity: PRKCA (NP_002728, 563 a.a. ~ 672 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone 2F11
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details PKC alpha

Product Details anti-PKC alpha Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRKCA (PKCa Antibody Abstract)
Background

Other Names: Protein kinase C alpha type, PKC-A, PKC-alpha, PRKCA, PKCA, PRKACA

Gene Description: protein kinase C, alpha

Gene Summary: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq

Molecular Weight 76764 DA
Gene ID 5578
NCBI Accession NP_002728
UniProt P17252
Pathways WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-PKC alpha Antibody Target Details PKC alpha Handling Images back to top
Application Notes Western blot = 1:500-1000
Restrictions For Research Use only

Handling

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Images back to top
Buffer Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

Images

Product Details anti-PKC alpha Antibody Target Details PKC alpha Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Protein Kinase C, alpha (PKCa) (AA 563-673) antibody (ABIN393606) Immunofluorescence of monoclonal antibody to PRKCA on HeLa cell (antibody concentrati...
Immunohistochemistry (IHC) image for anti-Protein Kinase C, alpha (PKCa) (AA 563-673) antibody (ABIN393606) anti-Protein Kinase C, alpha (PKCa) (AA 563-673) antibody (Image 2)
Western Blotting (WB) image for anti-Protein Kinase C, alpha (PKCa) (AA 563-673) antibody (ABIN393606) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Western Blotting (WB) image for anti-Protein Kinase C, alpha (PKCa) (AA 563-673) antibody (ABIN393606) PRKCA monoclonal antibody (M01), clone 2F11 Western Blot analysis of PRKCA expression...
Immunohistochemistry (IHC) image for anti-Protein Kinase C, alpha (PKCa) (AA 563-673) antibody (ABIN393606) Immunoperoxidase of monoclonal antibody to PRKCA on formalin-fixed paraffin-embedded ...
Immunofluorescence (IF) image for anti-Protein Kinase C, alpha (PKCa) (AA 563-673) antibody (ABIN393606) Immunofluorescence of monoclonal antibody to PRKCA on HeLa cell. [antibody concentrat...
ELISA image for anti-Protein Kinase C, alpha (PKCa) (AA 563-673) antibody (ABIN393606) Detection limit for recombinant GST tagged PRKCA is approximately 0.1ng/ml as a captu...
 image for anti-Protein Kinase C, alpha (PKCa) (AA 563-673) antibody (ABIN393606) Proximity Ligation Analysis of protein-protein interactions between FAS and PRKCA HeL...